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Biomedical subjects

J H Brewer

Publications and source records attributed to J H Brewer.

35 records · Page 2Linked to original sources
PubMed1980

Anaerobic microbiology in the NASA space program.

After briefly reviewing the earlier methods used to monitor the microbial load of returned lunar material, the author reports the more accurate research on the ability of terrestrial organisms to grow under simulated Martian environments. The possible importance of anaerobic microbiology can readily be seen because of the low level of O2 found on Mars. The question of whether any of the experiments on board the Viking landers show any indication of life on Mars is discussed in detail.

Anaerobiosis↗
PubMed1977

Isolation and characterization of omnitherms and facultative anaerobes from Cape Canaveral soil samples.

In a bacterial population profile of interplanetary spacecraft environments based upon temperature and oxygen requirements, several isolates demonstrated the ability to grow over a temperature range from 3 degrees C to 55 degrees C, and most grew aerobically and anaerobically. Because of the adaptability of these organisms, they may be of significance to planetary contamination. To verify that these were actively growing in this range of temperatures, they were streaked onto trypticase soy agar (TSA) and incubated at the extreme temperatures (3 degrees C and 55 degrees C). Isolated colonies were transferred to fresh TSA and immediately incubated at the opposite extreme temperatures. Almost all of the isolates grew quite well at both temperatures. Because these have been subcultured numerous times and still possess the ability to grow over a broad temperature range, this appears to be a stable characteristic. Many of these isolates possess the ability to grow anaerobically at 3, 32 and 55 degrees C. All of these organisms are sporeformers, and data are presented concerning their heat resistance and biochemical activity.

Aerobiosis↗
PubMed1968

A disposable anaerobic system designed for field and laboratory use.

A disposable anaerobic system which is characterized by its light weight and its compactness is described. The system consists of a multilayer plastic bag with a unique sealing device. A collapsible impregnated cardboard container is fitted with a catalyst and holders for a disposable hydrogen generator and an anaerobic indicator. The catalyst is active at room temperature and requires no heat activation. This system, which lends itself readily to compact storage, quick assembly, and ease of operation, is disposable after use.

Bacteria↗
PubMed1966

Safe Self-contained Carbon Dioxide-Hydrogen Anaerobic System.

An anaerobic system is described which allows the microbiologist or hospital technician ease of operation not previously possible with other systems. Gas cylinders, vacuum pumps, valves, and gauges have been eliminated. A new anaerobic lid was developed that is fitted only with a snap-in rubber gasket, a double stainless-steel gauze flash arrestor, and a catalyst holder. The holder contains a palladium pellet catalyst which is active at room temperature and requires no heat for activation. This system was made specifically for use with a disposable hydrogen-carbon dioxide generator and a disposable methylene blue anaerobic indicator. In addition to ease of operation, this jar incorporates unique safety features which eliminate the possibility of a laboratory explosion. An oxygen-free atmosphere composed primarily of nitrogen and carbon dioxide was quickly achieved within the jar to insure maximal growth.

Journal Article↗