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Biomedical subjects

J Gu

Publications and source records attributed to J Gu.

At least 325 records · Page 18Linked to original sources
PubMed1989

Atrial natriuretic peptide and its messenger ribonucleic acid in overloaded and overload-released ventricles of rat.

To study the occurrence of atrial natriuretic peptide (ANP) in overloaded ventricles, suprarenal aortic coarctation was performed on adult rats (n = 60) to induce overloaded left ventricle. In 36 rats, the overloads were released in 2 weeks. The hearts (6 rats in each group) were examined 2, 7, and 14 days after overload and 2, 7, 19, 47, and 77 days after overload release. Another group of 6 rats was coarctated for the second time for 1-6 days after 14 days of coarctation and 7 days of release. ANP immunoreactivity was examined by light and electron microscopic immunocytochemistry. ANP mRNA was studied by RNA-RNA tissue in situ hybridization. ANP immunoreactivity and ANP mRNA were found in the left ventricle 2 days after coarctation, and their amounts increased in proportion to the intraventricular pressure and the duration of coarctation. Two days after release, ANP and its mRNA began to decrease, but at 77 days ANP-containing granules still existed in the ventricular myocytes, whereas its mRNA became undetectable. The second coarctation triggered the release of the remaining ventricular ANP from the first overload and induced another cycle of increased ANP synthesis. Specific granules that do not contain ANP were found in overload-released ventricles, indicating the possible existence of other peptide hormones. These findings suggest that the extents of ventricular ANP gene expression and ANP synthesis and release are regulated by intraventricular pressure, and the occurrence of ANP in overloaded ventricles may not be a transient, immediately reversible phenomenon.

Animals↗
PubMed1988

A study of myc-related gene expression in small cell lung cancer by in situ hybridization.

The expression of myc-related genes (c-myc, N-myc, and L-myc) in small cell lung cancer (SCLC) was studied by RNA-RNA tissue in situ hybridization. The tissues investigated included cytospins of ten cell lines derived from patients with SCLC, four corresponding nude mouse xenografts from cell lines, and metastatic tumor tissue obtained by surgical biopsy and at autopsy. The probes were prepared as 35S labeled complementary RNA. The expression of each gene was demonstrated specifically by autoradiography in the cytoplasm of the neoplastic cell samples. The average levels of oncogene expression in each specimen corroborated previous data obtained by Northern blot assays. In addition, heterogeneity in gene expression from cell to cell in each sample was noted. This study represents the first attempt to demonstrate oncogene expression in lung cancer cell lines and tissues in situ, and confirms that the expression of these myc related genes can be seen in the primary tumor. The technique of RNA-RNA tissue in situ hybridization has great potential in answering fundamental questions of tumor cell heterogeneity and progression in SCLC. It should be useful in both prospective and retrospective studies.

Carcinoma, Small Cell↗
PubMed1987

Pharmacokinetics of norethisterone in humans.

The pharmacokinetics of a dose of 1mg norethisterone administered with 50 micrograms ethynyloestradiol was studied in 83 subjects. The dose was rapidly absorbed and there were wide variations in the serum NET concentrations at any particular time after dosing; the concentrations at 24 h varied from 100 to 1700 pg/ml. There was a significant negative correlation between the serum NET concentration and the time after dosing in all women. There were large inter-subject variations in the pharmacokinetic parameters, the elimination half-life and bioavailability showing 3- and 5- fold variability, respectively. Mean values for the parameters were t1/2, 7.6 h; bioavailability, 53.6 ng/ml/h; C max, 4.63 ng/ml; clearance, 22.6 l/h; and Vd 2361. There were a number of statistically significant correlations between the pharmacokinetic parameters and analysis of the correlations suggested that clearance was an important determinant of the bioavailability and of C max whereas the elimination half-life was the determinant of the NET concentration at 24 h. The pharmacokinetics of NET are compared with those of ethynyloestradiol. The wide variation in pharmacokinetics is likely to be important in determining inter-subject variations in efficacy and, particularly, side-effects of oral contraceptives especially now that low-dose formulations are widely used.

Biological Availability↗
PubMed1986

Occurrence, distribution and origin of peptide-containing nerves of guinea-pig and rat male genitalia and the effects of denervation on sperm characteristics.

A systematic immunohistochemical and radio-immunological survey of the occurrence, distribution and origin of the peptidergic nerve supply in guinea-pig and rat male genitalia is presented. Neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), peptide histidine isoleucine (PHI), substance P and CGRP were detected in the genital organs of both species. The densities and distribution patterns of the peptidergic nerves were compared with those of the adrenergic nerves, as revealed by antibodies raised against dopamine-beta-hydroxylase (D beta H) and tyrosine hydroxylase (TH), and the general neuronal component, as revealed by antibodies raised against neurofilament proteins (NF). Bilateral transection of the hypogastric nerves, in the guinea-pig, resulted in a decrease of substance P-containing nerves in the vas deferens and of NPY-, PHI- and VIP-containing nerves in the seminal vesicle. Unilateral disconnection of the pelvic nerves caused a decrease of VIP, PHI, substance P and CGRP nerve supply in the ipsilateral vas deferens and cauda epididymidis in the guinea-pig. A marked reduction of noradrenergic and NPY-containing nerves was observed in the vas deferens and sexual accessory glands of rats, chemically sympathectomised by chronic injection of low doses of guanethidine. Conversely, increase of substance P and CGRP immunoreactivities were observed, particularly in the vas deferens. After guanethidine, the cauda epididymidis and vas deferens were distended with spermatozoa, suggesting paralysis of the ducts. Spermatozoa had a decreased percentage of attached cytoplasmic droplets, indicating prolonged retention in the ducts.

Animals↗
PubMed1985

Cytochemical relationships in the paracervical ganglion (Frankenhäuser) of rat studied by immunocytochemistry.

Vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and calcitonin gene-related peptide (CGRP) immunoreactivities have been demonstrated in the paracervical ganglion of the rat using immunocytochemistry. Dopamine beta-hydroxylase (D beta H) and neurofilament protein triplet immunoreactivities have also been demonstrated in this region. The VIP, NPY, D beta H and neurofilament immunoreactivities were located in ganglion cells and nerve fibres, while CGRP immunoreactivity was localized only in nerve fibres. Many cells immunoreactive with D beta H antiserum were also immunoreactive with NPY antiserum. A small number of cells immunoreactive with VIP antiserum were also immunoreactive with NPY antiserum. CGRP-immunoreactive nerve fibres were distributed in certain regions of the ganglion only.

Animals↗
PubMed1985

Bombesin-like immunoreactivity in female rat genito-urinary tract.

The quantitative distribution and localization of bombesin-like immunoreactivity (BLI), by antiserum which cross-reacted fully with gastrin-releasing peptide (GRP), was determined by radioimmunoassay and immunocytochemistry in various regions of female rat urogenital tract. The highest concentration of BLI was found in the vagina, with lower but significant concentrations in the uterus and bladder. Bombesin-like immunoreactive nerve fibres were localized by immunocytochemistry in the smooth muscle layer, around blood vessels and in the submucosa of the vagina and bladder. BLI was separable into two immunoreactive peaks on both gel permeation chromatography and reverse phase HPLC. The major peak emerged exactly in the position of neuromedin C (C-terminal decapeptide of GRP) in both chromatographic systems, while the remaining immunoreactivity emerged close to the position of porcine GRP on gel permeation chromatography, but was easily distinguishable from this on HPLC. This suggests a significant species difference between rat and porcine GRP. Amphibian bombesin and mammalian neuromedin C have previously been shown to have potent biological actions in the rat urogenital tract. The presence of mammalian bombesin-like peptides in nerve fibres of the urogenital tract suggests that they may have a regulatory role in these organs.

Animals↗
PubMed1985

Antibodies to neurofilament protein and other brain proteins reveal the innervation of peripheral organs.

Monoclonal and polyclonal antibodies to neurofilament proteins, neuron-specific enolase, glial fibrillary acidic protein and S-100 have been used to demonstrate nerves, ganglion cells and the supportive glial system of the innervation of various organs. The female genitalia, the urinary tract, the respiratory system, the pancreas, the heart and the skin of several mammalian species, including rat, mouse, guinea pig, cat, pig, monkey and man were fixed in para-benzoquinone and portions of each organ were snap frozen. Serial or free-floating thick cryostat sections were stained using indirect immunofluorescence and peroxidase anti-peroxidase immunocytochemistry. In addition, the newly described and highly sensitive immunogold-silver staining technique was used on Bouin's-fixed and wax-embedded tissues. Antibodies to neurofilament proteins seemed to react with neuronal structures in all the species studied. Alternately stained serial sections showed a similar distribution of neurofilament proteins and neuron-specific enolase-containing nerves. Neuron-specific enolase staining had a diffuse appearance and was found to be highly variable, indicating that the neuron-specific enolase content might be related to the physiological state of the nerves and ganglion cells, whereas antibodies to neurofilament protein gave a consistently intense and very clear picture of the ganglion cells and nerve fibres. Antibodies to S-100 stained supportive elements of the peripheral nervous system in all tissues examined, whereas antibodies to glial fibrillary acidic protein were more selective.

Animals↗
PubMed1985

Peptide histidine methionine (PHM) and the human male genitalia.

Peptide histidine methionine-like immunoreactivity (PHM-IR) has been demonstrated to be present in the human penis both by radioimmunoassay and immunohistochemistry with particularly high levels in the corpus cavernosum and vas deferens. In the cavernosa, PHM-IR has been localised entirely, in nerves around arteries. High performance liquid chromatography indicated that this PHM-IR co-eluted with synthetic PHM but not porcine PHI. The presence of PHM-IR in the penis suggests that this neuropeptide may play a functional role in penile function.

Fluorescent Antibody Technique↗
PubMed1985

Stability of immunocytochemical reactivity of neuronal substances following delayed fixation.

The rates of deterioration of immunocytochemical staining for 4 peptide and 2 neuronal antigens were studied on human, guinea pig and rat colon and urinary bladder. It was found that if the fixation started within 10 h after surgery or death, little change was seen in the pattern and intensity of the immunostains. When fixation was further delayed, the immunostaining gradually deteriorated in quality and decreased in quantity until, when fixation was delayed for 60-72 h, most of the original immunostaining was absent. The deterioration rates of the neuronal antigens in the urinary bladder were slower than in the colon and all antigens were preserved for longer at 4 degrees C than at 20 degrees C. The deterioration curves produced from the present findings provide a standard for comparison with the immunostaining results obtained on tissues where fixation is delayed.

Animals↗
PubMed1985

Somatostatin in the human heart and comparison with guinea pig and rat heart.

Somatostatin has been shown to have negative inotropic and chronotopic effects and to restore sinus rhythm in some cases of cardiac arrhythmia. Using acid extracts, regions of human heart were examined by radioimmunoassay to determine their somatostatin content. Mean (SD) concentrations of 4.1 (0.8) pmol/g and 2.9 (0.8) pmol/g were found in atrioventricular node and right atria respectively and were significantly higher than in other heart regions. Using fresh heart tissue from guinea pigs, somatostatin was localised to cardiac nerves by immunocytochemistry. Nerves containing somatostatin were most abundant in the atria, where the concentrations measured by radioimmunoassay were 7.6 (1.0) and 2.6 (0.4) pmol/g for right and left atria respectively. Somatostatin contained in cardiac nerves may have a physiological role in the cardiac conduction system.

Animals↗
PubMed1984

Neuropeptide Y in the human male genital tract.

Neuropeptide Y (NPY) was found in high concentrations in the male genital tract. NPY levels were highest in the seminal vesicles, prostate, corpus cavernosum and vas deferens, where large numbers of immunoreactive nerve fibres were detected. Considerable quantities were also found in the epididymis and spongiosum. Lower concentrations were found in the glans penis, testis and foreskin. The presence of a large number of nerves containing NPY suggest that this active neuropeptide may play a role in control of genital function.

Adult↗
PubMed1984

Demonstration of paracervical ganglion origin for the vasoactive intestinal peptide-containing nerves of the rat uterus using retrograde tracing techniques combined with immunocytochemistry and denervation procedures.

The origin of the abundant vasoactive intestinal peptide (VIP)-immunoreactive nerves in the uterus has not been fully determined. In this study, a fluorescent dye, True Blue was injected into the uterus of rat and 6 days later, neuronal cell bodies of the paracervical ganglion were found to be labelled by this dye. Some of these labelled ganglion cells were also found to contain VIP immunoreactivity by immunocytochemistry. When the preganglionic pelvic and/or hypogastric nerves of rats were sectioned, the VIP-immunoreactive nerves in the uteri were not depleted, indicating that these nerves did not originate from the splanchnic ganglion, dorsal root ganglion or the spinal cord. Therefore it is concluded that VIP-immunoreactive nerves in the uterus originate from the paracervical ganglion.

Animals↗
PubMed1984

PHI-like immunoreactivity in the gallbladder and in vitro effect of porcine PHI on smooth muscle of the gallbladder.

The occurrence and distribution of PHI-like immunoreactivity in the guinea pig gallbladder has been analysed by radioimmunoassay and immunocytochemistry. Chromatography of gallbladder extracts by gel permeation and high-performance liquid chromatography revealed that guinea pig PHI-like immunoreactivity is of a similar size to that of porcine PHI but may differ in its amino acid sequence. Immunocytochemistry showed PHI-immunoreactivity to be localised to nerves found predominantly in the ganglionated plexus and the mucosal plexus of the gallbladder. Pure natural porcine PHI induced a dose-dependent relaxation of the isolated guinea pig gallbladder muscle which was not blocked by antagonists to acetylcholine, catecholamines, histamine, and 5-hydroxytryptamine. PHI may thus be one of the local factors involved in controlling gallbladder function.

Animals↗
PubMed1984

Decrease of vasoactive intestinal polypeptide (VIP) in the penises from impotent men.

Vasoactive intestinal polypeptide (VIP)-containing nerves were depleted in the penises of 28 impotent men. The extent of the decrease in VIP-containing nerves broadly reflected the severity of erectile dysfunction. VIP-immunoreactive nerves were most depleted in those men with complete erectile impotence, irrespective of the aetiology of the dysfunction, whereas in those men in whom some erectile function persisted, loss of VIP-immunoreactive nerves was more variable and less complete. Conventional histology demonstrated only mild changes (muscle atrophy and occasional thickening of blood vessel walls). VIP levels, measured by radioimmunoassay of tissue extracts, were depleted by more than 80% in penises from impotent diabetics (43.4 +/- 9.9 pmol/g wet weight [mean +/- SEM]), when compared with 6 controls (189.9 +/- 45.9 pmol/g). These findings not only support the contention that VIP may be the principal neurotransmitter involved in penile erection, but also suggest that depletion of this powerful vasodilatory peptide may play a key role in the development of penile impotence.

Adult↗
PubMed1984

Neuropeptide Y in the guinea-pig biliary tract.

High concentrations of neuropeptide Y (NPY) have been demonstrated in the gall bladder (16.7 +/- 5.4 pmol/g), cystic duct (25.4 +/- 9.2 pmol/g) and common bile duct (54.7 +/- 11.5 pmol/g) of the guinea-pig using a recently developed radioimmunoassay. Immunoreactive NPY containing nerves were demonstrated in all layers of the biliary tree using immunocytochemistry, being particularly dense in the myenteric and mucosal plexuses.

Animals↗