A transmissible resistance element from a strain of Pseudomonas aeruginosa containing no detectable extrachromosomal DNA.
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Biomedical subjects
Publications and source records attributed to J Grinsted.
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RP1, a group of genes specifying resistance to carbenicillin, neomycin, kanamycin, and tetracycline and originating in a strain of Pseudomonas aeruginosa, was freely transmissible between strains of P. aeruginosa, Escherichia coli, and Proteus mirabilis. Acquisition of the multiple drug resistance specified by RP1 by these strains was accompanied by acquisition of an extrachromosomal satellite of covalently closed circular deoxyribonucleic acid of molecular weight about 40 million daltons and of buoyant density 1.719 g/cm(3) (60% guanine plus cytosine).
RP4, an R factor derived from Pseudomonas aeruginosa S8 and specifying resistance to carbenicillin, neomycin, kanamycin, and tetracycline, could be isolated as an extrachromosomal satellite of covalently closed circular DNA (CCC-DNA) of molecular weight about 62 million and of buoyant density 1.719 g/cm(3) (60% guanine plus cytosine). When RP4 was compared by hybridization with RP1, an R factor originating in another strain of P. aeruginosa, it was shown that RP4 contains most, if not all, of the base sequences of RP1, and also additional sequences not found in RP1.
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