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Biomedical subjects

J Grange

Publications and source records attributed to J Grange.

35 records · Page 2Linked to original sources
PubMed1987

Association between Km1 immunoglobulin allotype and pulmonary tuberculosis in Indonesians.

Allotypes were determined in 121 cases of smear-positive pulmonary tuberculosis and 33 healthy controls from Indonesia. It was found that the occurrence of Km1 was significantly lower in patients than in controls (p = 0.011), and that phenotypes lacking G1m (17) and G3m(21) as well as Km1 occurred much more frequently among patients than controls (p = 0.0025). Some evidence for an allotypic influence on the antibody response to mycobacterial antigens was found. A lack of G1m(17) or G3m(21) was associated with increased antibody levels in the IgG2 subclass in control subjects and a lack of Km1 with decreased antibody levels in the IgG4 subclass in patients.

Antibodies, Bacterial↗
PubMed1986

Subdivision of daughter strains of bacille Calmette-Guérin (BCG) according to secreted protein patterns.

In order to identify proteins secreted by live organisms, daughter strains of the Bacillus Calmette-Guérin (BCG) were grown for 4-7 d in a defined medium containing [35S]methionine. Secreted components were then separated by polyacrylamide gel electrophoresis under both denaturing and non-denaturing conditions, and analysed by autoradiography and in an Ambis beta-scanner. The results indicate that BCG daughter strains can be subdivided into two groups according to their secretion of a 46 kDa protein dimer consisting of two similar 23 kDa subunits. High-producer strains (Japanese, Brazilian and Russian) secrete very large quantities of this material, which constitutes approximately 23% of all secreted protein. These findings correlate with earlier studies in which degradation products of the protein dimer may have been identified, and with the data from patterns of cell wall lipids.

Bacterial Proteins↗
PubMed1982

Bacteriological survey of tuberculous lymphadenitis in South-east England: 1973-80.

During the eight years 1973-80 the Public Health Laboratory Service Regional Centre for Tuberculosis Bacteriology received cultures of mycobacteria from 2339 patients with tuberculous lymphadenitis. Of these, 2272 were M tuberculosis (2207 human and 65 bovine strains) and 67 were other mycobacterial species, usually M avium and its intracellulare variant. Disease due to the human strains of M tuberculosis occurred most often in young women of Asian ethnic origin. Many bovine strains isolated from Asian patients differ from the classical bovine type in being sensitive to pyrazinamide: the origin of these strains, whether from other people or from cattle, is unknown. Lymphadenitis due to bovine strains tended to occur in an older age group than the human strains and probably include relatively more cases of reactivation diseases. Infection caused by the other mycobacterial species occurred mainly in young children of European origin. Tuberculosis, therefore, remains an important cause of lymphadenopathy in Britain.

Adolescent↗
PubMed1979

[Interaction between an antifungal heptaene, amphotericin B and cholesterol in vitro, as detected by circular dichroism and absorption. Influence of temperature].

The polyene antibiotic "Amphotericin B" can interact with sterols, cholesterol or ergosterol in aqueous and hydroalcoholic media and a correlative striking spectral change appears between 300 and 420 nm in the CD and absorption spectra. Using these spectroscopic methods we have determined that the influence of temperature between 4 and 80 degrees C is very important. The higher the temperature the most rapid is the modification of the spectra. Thus, the "Amphotericin B"-sterol complex is more easily formed when heating. We have not found any reversibility at 80 degrees C.

Amphotericin B↗
PubMed1978

The preparation of latex particles with covalently bound polyamines, IgG and measles agglutinins and their use in visual agglutination tests.

Carboxylated latex particles were substituted with side arms terminating in primary amine and hydrazine groups. The particles were coupled to aldehyde groups generated on glycoproteins which were treated with sodium periodate. Particles having the alipathic primary amine putrescine hapten as the sole substituent and particles linked to glycoproteins such as measles agglutinins and IgG were used to detect the presence of the corresponding antibodies or antigens in biological fluids by agglutination tests.

Agglutination Tests↗
PubMed1977

Nephelometric assay of antigens and antibodies with latex particles.

Antigens and antibodies covalently bound to latex particles have been used to detect specifically corresponding antibodies and antigens by nephelometry. A systematic study of factors such as wavelength, angle of observation, concentration of latex particles, and reaction time has permitted us to develop a procedure suitable for routine estimation of antigens and antibodies in the nano and picogramme range.

Antibodies↗
PubMed1972

Composition and size of Shope fibroma virus deoxyribonucleic acid.

Deoxyribonucleic acid (DNA) extracted from purified virions of Shope fibroma virus (SFV) (by using DNA from Microccocus lysodeikticus as marker) had a buoyant density of 1.6996 +/- 0.0003 g/ml), hence a guanine plus cytosine (G + C) content of 40.4 +/- 0.3%, which is close to the G + C content of the DNA of susceptible rabbit cells (40.9 +/- 0.4%) and different from that of vaccinia virus DNA (35.5 +/- 0.4%). For the determination of the molecular weight of DNA, SFV and vaccinia purified virions, treated with Pronase and detergent, were cosedimented in sucrose density gradients. Results showed that SFV-DNA has a molecular weight of about 153 x 10(6) daltons. By electron microscopy, only one molecule corresponding to this value was observed (its length was 80.3 mum). The others had a median size of 49.8 mum +/- 0.9.

Animals↗
PubMedDate not supplied

[Specific monoclonal antibodies to the Mycoplasma-type pathogenic agent of grapevine flavescence dorée].

Three hybridomas producing monoclonal antibodies specific for the mycoplasma-like organism (MLO), pathogenic agent of grapevine flavescence dorée, were obtained after fusion between spleen cells from a mouse immunized against flavescence dorée MLO and Sp2/O-Ag-14 mouse myeloma cells. These hybridomas were selected in an indirect sandwich ELISA in which antibodies from two different animal species were used (rabbit and mouse). This assay is convenient for anti-MLO monoclonal antibody screening, because of its sensitivity, specificity and good preservation of antigens. The three monoclonal antibodies were examined for reactivity towards the MLO causal agents of 15 plant yellows. None of the three were implicated in a serological relationship between the MLO of these plant yellows and flavescence dorée-MLO. Reactivity of the three monoclonal antibodies was checked towards two other isolates of flavescence dorée. One of the three antibodies detected the wild isolates.

Antibodies, Bacterial↗