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Biomedical subjects

J Goto

Publications and source records attributed to J Goto.

At least 253 records · Page 14Linked to original sources
PubMed1978

A new method for simultaneous determination of bile acids in human bile without hydrolysis.

A method for simultaneous determination of major bile acids in human bile without prior hydrolysis is described. The unconjugated, glycine- and taurine-conjugated bile acids are separated into groups by ion-exchange chromatography on a newly developed lipophilic gel, piperidinohydroxypropyl Sephadex LH-20 (PHP-LH-20). Subsequently, resolution of each group into cholate, ursodeoxycholate, chenodeoxycholate, deoxycholate and lithocholate is attained into two stages by high-performance liquid chromatography on a micron-Bondapak C18 column. First, 0.3% ammonium carbonate/acetonitrile (9 : 4, v/v) is used for separation of the latter three as a mobile phase. Cholate and ursodeoxycholate are separated in 0.3% ammonium carbonate/acetonitrile (11 : 4, v/v). The present method is applicable to quantitation of free and conjugated bile acids in human bile with satisfactory accuracy and precision.

Bile↗
PubMed1978

Studies on steroids. CXXVIII. Separation and determination of bile acids by high-performance liquid chromatography.

A method is described for the simultaneous determination of major bile acids by high-performance liquid chromatography without prior hydrolysis. A mixture of bile acids is divided into the free, glyco- and tauro-conjugate groups by thin-layer chromatography. Separation of each group into cholate, ursodeoxycholate, chenodeoxycholate, deoxycholate and lithocholate is attained in two stages on a muBondapak C18 column; first, 0.3% ammonium carbonate-acetonitrile (9:4) is used as a mobile phase for the separation of the last three compounds. Subsequently cholate and ursodeoxycholate are resolved by chromatography in 0.3% ammonium carbonate-acetonitrile (11:4).

Bile Acids and Salts↗
PubMed1978

Separation of sulfated bile acids by high-performance liquid chromatography.

In order to establish a new method for stimultaneous determination of sulfated bile acids without deconjugation, separation of 3-sulfates of unconjugated, glyco- and tauroconjugated bile acids by high-performance liquid chromatography has been undertaken. The preliminary experiment indicated that reversed phase chromatography on an ODS SC-02 column using ammonium carbonate aqueous solution/acetonitrile as a mobile phase would be promising. The ratio of peak width to retention time was varied depending upon the salt concentration. The content of water in mobile phase exerted an influence on the capacity ratio to a certain extent, but not on the resolution. The use of 0.5% ammonium carbonate/acetonitrile (26:8 and 20:8, v/v) was found to be suitable for complete resolution of sulfated cholate, chenodeoxycholate, deoxycholate, lithocholate and their glyco- and tauro-conjugates.

Bile Acids and Salts↗
PubMed1974

Distribution of monoamine-containing terminals and fibers in the central nervous system of the chicken.

In the mesencephalon of the chicken, the two large groups of CA terminals and fibers appeared to correspond to the dorsal and ventral bundles of the CA containing fibers in the rat. These ran rostrally in the medial forebrain bundle and appeared to terminate within the thalamus, hypothalamus, preoptic region and corpus striatum. 5-HT containing terminals and fibers also were observed in the mesencephalon and appeared to run rostrally in the medial part of the medial forebrain bundle. In comparison with the CA terminals, however, 5-HT terminals were for the greater part, difficult to observe in many areas of the brain. Based on the observation concerning postnatal ontogeny of monoamine-containing neurons, the chicken brain appears to acquire behavioral and functional maturity at an earlier stage after birth than does that of the rat. Effect of reserpine, nialnmide, L-Dopa and 5-HTP on the monoamine-containing terminals and fibers are briefly discussed.

Amines↗