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Biomedical subjects

J Glover

Publications and source records attributed to J Glover.

120 records · Page 7Linked to original sources
PubMedDate not supplied

The peroxidase antiperoxidase staining of factor VIII-related antigen on cultured endothelial cells.

To prevent the premature occlusion of vascular prostheses, endothelium is being cultured experimentally onto synthetic flow surfaces. A rapid method of identifying cultured endothelium on the prosthesis is valuable for determining the degree of fibroblast and smooth muscle cell contamination and to screen for endothelial cell transformation. Fluorescent Factor VIII related antigen (FVIII-RA) staining has been used to identify cultured endothelium, but results in excessive staining of the underlying prosthesis, loss of morphologic detail, and deterioration of the FVIII-RA antibody reaction with time. We have applied the peroxidase antiperoxidase (PAP) method of antigen staining to permit staining of FVIII-RA and thereby to permit a sensitive and specific identification of human or canine endothelium with a concurrent analysis of morphologic detail.

Animals↗
PubMedDate not supplied

The quantitation of cultured cellular surface coverage: applications for transparent and opaque surfaces.

A planimetric technique is described for the analysis of cellular linings cultured on vascular prostheses and tissue culture plates. The method is not destructive of the cells and uses a calibrated eyepiece grid and an inverted microscope. The technique allows a rapid assessment of the percent of the prosthetic surface covered by cells, the average size of the cellular islands, and their distribution. In addition, the cells may be counted, their average surface area computed, and growth curves constructed for cells grown on tissue culture plates without the need for multiple concurrent cultures. Different cell types may be counted on the same surface. The method is more accurate than methods that require the resuspension of cells for counting, and it saves time and cells.

Animals↗
PubMedDate not supplied

Changes in taste associated with intravenous administration of pentamidine (revised)

In this pilot study the authors set out to: (a) describe the incidence of taste changes associated with intravenous (i.v.) pentamidine treatment; and (b) determine the factors that are related to making the taste changes better and worse. A convenience sample (N = 18) of adult male outpatients with AIDS participated in this study. One hundred percent of the participants experienced an unpleasant taste after the administration of pentamidine. The largest percentage of patients had a metallic taste (89%, n = 16); while 67% (n = 12) experienced a bitter taste. These data suggest that healthcare providers need to assess for the unpleasant taste associated with i.v. pentamidine and develop appropriate management strategies.

AIDS-Related Opportunistic Infections↗
PubMedDate not supplied

Changes in taste associated with intravenous administration of pentamidine.

In this pilot study the authors set out to: (a) describe the incidence of taste changes associated with intravenous (i.v.) pentamidine treatment; and (b) determine the factors that are related to making the taste changes better and worse. A convenience sample (N = 18) of adult male outpatients with AIDS participated in this study. One hundred percent of the participants experienced an unpleasant taste after the administration of pentamidine. The largest percentage of patients had a metallic taste (89%, n = 16); while 67% (n = 12) experienced a bitter taste. These data suggest that healthcare providers need to assess for the unpleasant taste associated with i.v. pentamidine and develop appropriate management strategies.

AIDS-Related Opportunistic Infections↗
PubMedDate not supplied

The effects of crimping on the healing of prosthetic arterial grafts.

The purpose of this study was to determine whether the crimping of arterial prostheses affected their healing. In ten dogs, 6 mm knitted Dacron grafts were implanted in the infrarenal aortas; 6 grafts were crimped, 4 uncrimped. Each was seeded with venous endothelium to produce a cellular lining. The grafts were removed a month after implantation and compared by light microscopy as to the (1) thickness of the inner lining, (2) completeness of endothelial healing, and (3) patterns of vasa vasorum. (1) and (3) were assessed using H & E sections and (2) by AgNO3 Häutchen preparations. The lining in uncrimped grafts was thinner and more uniform than in crimped grafts: 50.1 +/- 45.7 vs 263 +/- 183 mu, p < 0.001. While the completeness of healing was similar for both groups, 4 crimped grafts had unhealed areas in the centers of the crimps, corresponding to the thicker areas of the linings. In both kinds of grafts, vasa vasorum communicated with vascular channels parallel to the axis of flow and near the perigraft fat. In the uncrimped grafts, the smaller secondary vasa also were directed along the axis of flow, in contrast to crimped grafts in which they were circumferential to the graft and parallel to the crimps. In all instances vasa penetrated within 500 mu of the lumen. We conclude that crimping causes a change in the pattern of development of vasa vasorum in healing fabric grafts and a thicker inner lining associated with areas of delayed healing which may increase the risk of thrombosis.

Animals↗
PubMedDate not supplied

Small cell lung cancer: pathophysiologic mechanisms and nursing implications.

PURPOSE/OBJECTIVES: To review current incidence and treatment perspectives for small cell lung cancer (SCLC). DATA SOURCES: Published articles, book chapters, American Cancer Society materials. DATA SYNTHESIS: SCLC constitutes 20%-30% of lung cancer cases and accounts for 40,000 deaths annually. Characterized by rapid cell division, SCLC is very sensitive to radiation and chemotherapy; however, long-term survival rates are very low. A number of paraneoplastic syndromes are associated with this disease, thereby increasing the medical and nursing management challenges. CONCLUSIONS: Because SCLC usually is diagnosed late in the disease process and survival rates are poor, quality of life and palliation of metastatic symptoms (e.g., pain, breathing difficulties) are important focuses of care. Smoking deterrence and cessation programs, as well as early detection of lesions, are key to reducing incidence and mortality. IMPLICATIONS FOR NURSING PRACTICE: Nursing care focuses on promoting comfort and preventing and managing disease and treatment complications. Nursing diagnoses include ineffective airway clearance, impaired gas exchange, pain, altered nutrition, activity intolerance, and ineffective coping. A patient care standard is presented.

Age Factors↗
PubMedDate not supplied

A professional code of ethics: providing a moral compass for turbulent times.

PURPOSES/OBJECTIVES: To describe the importance of a professional code of ethics and values in guiding and supporting the practice of nurses and to present the proposed Oncology Nursing Society (ONS) Statement of Core Values. DATA SOURCES: Published articles and books on nursing ethics and bioethics, as well as authors' clinical experiences. DATA SYNTHESIS: Ethical quandaries are commonplace for nurses, and the contemporary context of health care has created new concerns and redefined others. Throughout its history, nursing has been an ethical endeavor, with nurses attempting to sift through complex ethical issues and fulfill their professional responsibilities. A professional code of ethics and values explicates the goals and norms of the profession and provides direction for practice. CONCLUSION: Together, the profession's code of ethics and the proposed ONS Statement of Core Values serve as a resource to guide oncology nurses as they develop an ethically competent practice and confront contemporary ethical challenges. IMPLICATIONS FOR NURSING PRACTICE: The goal of developing an ethically competent practice, maintaining professional integrity, and rendering quality patient care is of vital concern to oncology nurses. Familiarity with and commitment to the primary ethical precepts and values of the profession are essential to accomplishing this goal.

American Nurses' Association↗