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Biomedical subjects

J Gardner

Publications and source records attributed to J Gardner.

At least 325 records · Page 18Linked to original sources

PSO tug of war.

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Community Networks↗

A monumental task.

President Clinton is hoping to secure a place in history by balancing the federal budget, a prospect that has many in the healthcare industry worried. With Medicare and Medicaid eating up major portions of federal spending, any move to trim the deficit will require painful cuts in provider payments. And funds for programs like children's access to healthcare are likely to be scarce.

Budgets↗

Pols PAC it in.

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Data Collection↗

Use of recombinant vaccinia to restimulate antigen specific human peripheral blood cytotoxic T lymphocytes.

A simple method is described for using recombinant vaccinia virus for restimulating human peripheral blood mononuclear cells (PBMC) to generate antigen specific CD8 + alpha beta cytotoxic T cell (CTL) effector populations. Effector PBMC were restimulated in vitro with a subpopulation of PBMC, which had been infected with recombinant vaccinia at very low multiplicities of infection in the absence of serum. This protocol avoided the vaccinia mediated overt cytopathic effects on the effector PBMC and generated bulk CTL cultures, which could be used for the identification of epitopes recognised by antigen specific CTL.

CD8-Positive T-Lymphocytes↗