An unusual case of extraarticular lipoma of the knee joint.
Lipoma of the knee joint is a rare disease and most cases are lipoma arborescens within the joint. Here we report a case of true extraarticular lipoma of the knee.
Adult↗
Biomedical subjects
J Gao
Publications and source records attributed to J Gao.
Detection and identification of Mycobacterium avium in the blood of AIDS patients by the polymerase chain reaction.
One hundred fifty-three blood samples from patients positive for the human immunodeficiency virus (HIV) were analyzed by polymerase chain reaction (PCR) to detect the presence of Mycobacterium avium. Samples were collected from patients who also had blood cultures performed by a radiometric method. Blood samples were centrifuged on a Ficoll-Hypaque gradient to purify peripheral blood mononuclear cells. The purified cells were washed and incubated with a resin, boiled to release mycobacterial DNA, and then amplified. Polymerase chain reaction products were detected by a nonisotopic method. A 123 base-pair (bp) insertion sequence, namely IS6110, from Mycobacterium tuberculosis complex was also included in the reaction as an internal control of Taq polymerase activity to exclude the presence of enzyme inhibitors. This IS6110 fragment can be distinguished from the 383 bp target product on ethidium bromide-stained agarose gel and may also be used in a colorimetric assay. Such results were compared with the results of culture and indicated that the assay is as sensitive as bacteriological methods, though faster.
Acquired Immunodeficiency Syndrome↗
Decreased plasma membrane calcium transport activity in aging brain.
We have assessed the functional properties of both calmodulin (CaM) and the plasma membrane Ca(2+)-ATPase in brains of young, middle aged, and old Fisher 344 rats. Under optimal conditions of saturating Ca2+ and ATP, the CaM-activated Ca(2+)-ATPase activity was decreased with increasing age, particularly when CaM isolated from the brains of aged rats was used to stimulate the enzyme. In the case of CaM, structural modifications within the primary sequence of the protein from aged brains were identified. We found that during normal biological aging approximately 6 methionine residues were modified to their corresonding sulfoxide per CaM, and no other amino acids were modified. Some aspects of the age-related decline in the effectiveness of CaM as an activator of Ca(2+)-ATPase could be simulated using a range of reactive oxygen species (including hydrogen peroxide and oxoperoxynitrite) and, in the latter case, the extent of oxidative modification of specific methionine residues was directly related to their surface accessibility. The pattern of oxidative modification of the methionines in the aged CaM was less straightforward, though both in vitro oxidation of CaM and aging within the brain markedly decreased the functional properties of this important Ca(2+)-regulating protein.
Aging↗
Heat-killed Bacillus subtilis inhibits T-cell proliferative response to mitogens and recall antigens.
Heat-killed vegetative forms of Bacillus subtilis were found to impair considerably the capacity of human T-lymphocytes to secrete interleukin-2 (IL-2) and to proliferate (in terms of [3H]thymidine incorporation) after phytohaemagglutinin (PHA) stimulation. B. subtilis was also found to interfere with T-cell proliferation induced by concanavalin A (Con A) and the recall antigen tetanus toxoid (TT). The suppressive activity was dependent on bacterial concentration, and was not ascribed to mitogen, medium-nutrient absorption or cell killing. Moreover, B. subtilis did not interfere with mitogen-induced IL-2 receptor expression on the T-cell surface. On the other hand, B. subtilis did not interfere with T-cell proliferation induced by phorbol myristate acetate (PMA) and ionomycin stimulation. All data obtained suggest the binding of B. subtilis subcomponents to- or very close to-the T-cell receptor (TCR). Identification and purification of the basic structure(s) or component(s) of B. subtilis with TCR antagonist activity in vitro will help to exploit different aspects of T-cell activity and development, and possibly, will provide a means of specific control or modification of the immune response.
Bacillus subtilis↗
IFN-gamma restores HIV- and non-HIV-specific cell mediated immune response in vitro and its activity is neutralized by antibodies from patients with AIDS.
The addition of IFN-gamma to cultures of peripheral blood mononuclear cells (PBMCs) obtained from asymptomatic HIV-infected patients increased cell proliferation in response to HIV envelope synthetic peptides (Env), influenza A virus (VIRUS), and allogeneic lymphocytes (ALLO) but not to phytohaemagglutinin (PHA) stimulation. F(Ab)2 fragments of IgG purified from the sera of HIV-seropositive patients specifically interfered with IFN-gamma-induced cell proliferation in response to recall antigens. Neutralization of the lymphokine activity was found to be sustained by specific IFN-gamma antibodies. Data obtained demonstrate that IFN-gamma can restore the cell-mediated immunity of a number of asymptomatic HIV+ individuals in vitro, while IFN-gamma antibodies present in sera of patients with AIDS interfere with the activity of the lymphokine.
Acquired Immunodeficiency Syndrome↗
Natural healing of anterior and posterior attachments of the rabbit meniscus.
The natural healing capacity of the anterior or posterior medial meniscal attachment after radial transection and the effect of such procedures on knee joint cartilage were investigated in 28 skeletally mature New Zealand white rabbits. Either the anterior (n = 14) or the posterior attachment (n = 14) was radially transected in the right knee joint. Fifty percent of the rabbits with each procedure were euthanized at 6 weeks and 50% at 12 weeks. The histologic characteristics of the healing tissue were evaluated, including immunohistochemical demonstration of nerve fibers. Histologic changes in the synovium and articular cartilage were graded for severity and extent. The concentration of proteoglycan fragments in joint fluid was analyzed before operation and at euthanasia. After both procedures, the transected attachment healed in a prolongated position resulting in peripheral displacement of the meniscus. The healing tissue at 6 weeks was composed mainly of granular tissue. By 12 weeks, the healing tissue at the anterior attachment had a ligament like structure, but at the posterior attachment, fibrocartilage like tissue had formed. Nerve fibers were found in the healing tissue, but connections to nerves in normal attachment or meniscal tissues were not found. Increased concentrations of proteoglycan fragments, articular cartilage degeneration, and synovitis were found in the operated knees. These data indicate that although the meniscal attachment can heal in a prolongated position, such a meniscus has lost its mechanical functions. Further, a joint protective function of the neoneurons is doubtful because of the lack of continuity with the adjacent meniscus. Rigid fixation of the attachment seems essential for a functional meniscal substitute.
Animals↗
Relativistic electron moving in a multimode quantized radiation field.
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Journal Article↗
Immunolocalization of basic fibroblast growth factor (bFGF) in human periodontal ligament (PDL) tissue.
The distribution of basic fibroblast growth factor (bFGF) in periodontal ligament (PDL) tissue was investigated in samples which were obtained from freshly extracted human teeth. The PDL tissue was collected by scraping, and bFGF was identified and localized by immunohistochemistry. Fibroblasts, endothelial cells, some fibrocytes and extracellular matrix (ECM) stained positively for bFGF. It was observed that cells from healthy PDL stained more intensely than those from PDL of teeth associated with chronic periodontitis; histological cell counts revealed that the numbers of fibroblasts was greater (p < or = 0.0005) in healthy PDL than in diseased PDL tissue. The results of this study show that bFGF is produced primarily by PDL fibroblasts and endothelial cells in the PDL and that bFGF levels may be decreased in tissue associated with chronic periodontal lesions.
Adult↗
De novo methylation of CpG island sequences in human fibroblasts overexpressing DNA (cytosine-5-)-methyltransferase.
Recent studies showing a correlation between the levels of DNA (cytosine-5-)-methyltransferase (DNA MTase) enzyme activity and tumorigenicity have implicated this enzyme in the carcinogenic process. Moreover, hypermethylation of CpG island-containing promoters is associated with the inactivation of genes important to tumor initiation and progression. One proposed role for DNA MTase in tumorigenesis is therefore a direct role in the de novo methylation of these otherwise unmethylated CpG islands. In this study, we sought to determine whether increased levels of DNA MTase could directly affect CpG island methylation. A full-length cDNA for human DNA MTase driven by the cytomegalovirus promoter was constitutively expressed in human fibroblasts. Individual clones derived from cells transfected with DNA MTase (HMT) expressed 1- to 50-fold the level of DNA MTase protein and enzyme activity of the parental cell line or clones transfected with the control vector alone (Neo). To determine the effects of DNA MTase overexpression on CpG island methylation, we examined 12 endogenous CpG island loci in the HMT clones. HMT clones expressing > or = 9-fold the parental levels of DNA MTase activity were significantly hypermethylated relative to at least 11 Neo clones at five CpG island loci. In the HMT clones, methylation reached nearly 100% at susceptible CpG island loci with time in culture. In contrast, there was little change in the methylation status in the Neo clones over the same time frame. Taken together, the data indicate that overexpression of DNA MTase can drive the de novo methylation of susceptible CpG island loci, thus providing support for the idea that DNA MTase can contribute to tumor progression through CpG island methylation-mediated gene inactivation.
Base Sequence↗
Distal Sp3 binding sites in the hIGBP-1 gene promoter suppress transcriptional repression in decidualized human endometrial stromal cells: identification of a novel Sp3 form in decidual cells.
The purpose of this study was to identify the nuclear components that account for a dramatic transcriptional induction of the human insulin-like growth factor binding protein-1 (IGFBP-1) gene in decidualized human endometrial stromal cells. The study focused on the functional analysis and binding characteristics of the two distal promoter regions, 132 bp -2.732 to -2.6 kb [C/al/Xbal, (CX) fragment] and 30 bp [-2830 to -2800 bp, Hincll/ Sphl [HS] fragment]. In the primary culture of endometrial stromal cells, we have examined the three cis-elements, I, II and III, in the 58-bp BsaHl/ Rsal (BR) fragment (a promoter fragment that resides in CX) previously identified in HEC-1B cells. When pBRPL (BR 5'-linked to -2600 to +68 bp IGFBP-1 promoter/chloramphenicol acetyl transferase gene) was transfected into cells, only cis-element II (-2660 to -2638 bp) mediated the activation, whereas I and III (-2675 to -2666 bp and -2637 to -2628 bp) were inactive in both unstimulated and hormone-stimulated endometrial stromal cells. Promoter activity derived from pCXPL was repressed in unstimulated cells. During decidualization of endometrial stromal cells, the repression was gradually diminished, which was mediated by cis-element I and III. The binding pattern of the BR fragment analyzed by electrophoretic mobility shift assay using nuclear extracts of decidual tissue or cells showed three major specific binding complexes, C1, C2, and C3, which were abolished by a complementary oligonucleotide of the Sp1-binding motif dose-dependently. Only two complexes, C2 and C3, were formed by nuclear extracts of unstimulated stromal cells. A binding study with mutated BR fragment showed that the binding sites of C1, C2, and C3 resided at cis-elements I and III. The Sp3 antibody abolished complexes C1, C2, and C3. The Sp1 and Sp4 antibodies, however, had little effect. The other distal promoter region, 30-bp HS fragment, which contains a core sequence homologous to the Sp1-binding motif, formed a complex with mobility similar to C1. It is transcriptionally active only in decidualized stromal cells. The binding protein of this complex recognized the Sp3 antibody. Immunoblot analysis of the nuclear extracts of stromal and decidual cells showed that Sp3 antibody identified 120-, 82-, and 80-kDa Sp3 proteins. An additional 28-kDa Sp3 protein was present only in the extract of decidual cells. These findings suggest that decidual Sp3 protein may be responsible, at least in part, for the transcriptional regulation in decidualized endometrial stromal cells.
Adult↗
Interactions among Janus kinases and the prolactin (PRL) receptor in the regulation of a PRL response element.
PRL regulates milk gene expression, at least in part, by activating JAK2 kinase and STAT5 (signal transducer and activator of transcription 5), initially termed mammary gland factor (MGF). These experiments were initiated to gain a better understanding of the mechanisms of transcriptional activation via PRL receptor (PRL-R) signaling. Binding of PRL to the recombinant pigeon PRL-R-activated transcription driven by a 2.8 kbp 5'-fragment of the rat beta-casein gene. PRL enhanced the expression of chimeric reporters containing the beta-casein PRL response element (PRE), but not the c-fos sis-inducible element, when the reporters were transfected into Chinese hamster ovary cells with the PRL-R. Wild type receptor, which contains a duplication of the entire extracellular ligand-binding domain, was only slightly more effective than a truncation mutant with a single extracellular domain. Transfection with either JAK1, JAK2, or JAK3 increased basal transcription through both the PRE and sis-inducible element. Coexpression of JAK2 with PRL-R resulted in amplification of the induction of the PRE by PRL, whereas JAKs 1 and 3 did not amplify the PRL effect. Overexpression of JAK2 mutants blocked PRE activation by PRL. Mutant JAK2 also interfered with PRE activation by JAK3 but did not affect JAK1's stimulatory effect.
Animals↗
Identification and endocrine control of sex steroid binding sites in the lacrimal gland.
Previous research has indicated that the lacrimal gland may be a target organ for sex steroids and that androgen effects on this tissue may be inhibited by pituitary deficiency or diabetes. To extend these findings, the objectives of the current investigation were 3-fold: [a] to determine whether specific and high-affinity binding sites for androgens and estrogens exist in rat lacrimal tissue; [b] to assess whether the number and affinity of androgen binding sites in the lacrimal gland may be influenced by hypophysectomy or acute diabetes; and [c] to examine whether androgen receptor mRNA may be detected in lacrimal tissues of a variety of species. Following the collection of lacrimal gland samples, tissues were processed for the conduct of equilibrium binding methods or molecular biological techniques. Our results demonstrated that a single class of saturable, high-affinity and stereochemically selective binding sites for androgens exist in lacrimal tissues of male and female rats. These sites possessed a dissociation constant of approximately 1 nM and were also present in isolated acinar epithelial cells. In contrast, we were unable to find any evidence for the presence of specific or high-affinity receptors for estrogens in the rat lacrimal gland. With regard to changes in the endocrine environment, hypophysectomy led to an increase in the number and affinity of androgen binding sites in rat lacrimal tissue cytosol, whereas diabetes reduced the total quantity of these sites. Of interest, androgen receptor mRNA was detected in lacrimal glands of mice, rats, hamsters, guinea pigs, rabbits and humans. Overall, our findings show that the lacrimal gland is a target organ for androgens and that androgen action in this tissue may be mediated through an interaction with specific and high-affinity binding sites.
Adult↗
Concentrations of proteoglycan fragments in relation to maturation, sex and time of day: physiologic variations in knee joint fluid of rabbits.
We analyzed the concentrations of proteoglycan fragments in knee joint fluid in 142 rabbits to investigate the effect of physiologic variations--i.e., maturation, sex and time of day. The concentrations of proteoglycan fragments differed significantly between young, adolescent and adult animals and showed an inverse correlation to the stage of maturation of the rabbit. Adolescent male rabbits had higher concentrations than age-matched females. Morning and evening samples had similar concentrations. No relation was found between the proteoglycan fragment concentrations in joint fluid and the cartilage mass. The proteoglycan fragment concentrations in knee joint fluid apparently reflect the metabolic status of growing articular cartilage. There are considerable physiologic variations associated with maturation and sex, and these need to be taken into account when using the proteoglycan fragment concentration as a marker for joint diseases.
Age Factors↗
[Relationship between the state of intravascular bubbles and microcirculation system].
To confirm the hypothesis that air bubbles were unable to block the blood vessels and that the state of the intravascular bubbles was determined by the function of the circulatory system, 35 guinea pigs were pressurized then were decompressed to normal pressure. Microscopic observation was made of the bulbar conjunctival, dorsum auricular and subcutaneous vessels in 33 surviving animals. Air bubbles of different amounts, sizes and shapes were found in the dorsum auricular and subcutaneous vein of all the amimals and in the bulbar conjunctival oriridal artery of 16 animals, and in some cases the vessels were even filled with bubbles. The bubbles ran in the same direction and at the same speed as the blood flow. They could run in a backward, to-and-fro or sluggish flow. The bubbles looked shapeless and tended to break and divided into branch flows where the vessel branches. The bubbles were motionless at the proximal end of the artery occluded due to spasm or when the blood was stagnated. Under the action of the blood pressure the bubbles could expand the vessel and push forward. The bubbles showed a tendency of flowing with ease with the function of the vessel recovered. The results suggest that bubbles of any size in the vessel could easily change their shape under the action of the blood flow and pressure, and pass through vessels of any diameter and circulate with the blood. Only when a vessel was occluded due to spasm or the blood in a vessel was stagnated could the bubbles be motionless, but it was not that the bubbles blocked the vessel.
Animals↗
Quantitative comparison of soft tissue-bone interface at chondral ligament insertions in the rabbit knee joint.
At chondral ligament insertions the calcified fibrocartilage interdigitates deeply with the lamellar bone. The shape of this interface is formed under physiological loading conditions. For the purpose of morphological comparison between different ligament entheses in the rabbit knee, the number and frequency of interdigitations and thickness of calcified fibrocartilage were quantitated at the femoral insertion of the medial collateral ligament, both insertions of the cruciate ligaments, and the tibial insertion of the patellar ligament. Among the insertions, the femoral insertion of the medial collateral ligament showed the lowest frequency and depth of interdigitations at the soft tissue-bone interface, but had the thickest zone of calcified fibrocartilage. An inverse relationship was found at the insertion interface of the cruciate and patellar ligaments. The frequency and depth of interdigations at the bone-soft tissue interface at different chondral entheses seem to be related to the mechanical strength of the respective ligament; meanwhile it may be hypothesised that the thickness of the calcified fibrocartilage might be more related to the amount of motion which takes place at an insertion.
Animals↗
Reaction of hydroxyl radical with phenylpropanoid glycosides from Pedicularis species: a pulse radiolysis study.
Using pulse radiolysis technique, the reaction between hydroxyl radical and 7 phenylpropanoid glycosides: echinacoside, verbascoside, leucosceptoside A, martynoside, pediculariosides A, M and N which were isolated from Pedicularis were examined. The rate constants of these reactions were determined by transient absorption spectra. All 7 phenylpropanoid glycosides react with hydroxyl radical at high rate constants within (0.97-1.91) x 10(10)L.mol-1.s-1, suggesting that they are effective hydroxyl radical scavengers. The results demonstrate that the numbers of phenolic hydroxyl groups of phenylpropanoid glycosides are directly related to their scavenging activities. The scavenging activities are likely related to o-dihydroxy group of phenylpropanoid glycosides as well.
Drugs, Chinese Herbal↗
The morphology of ligament insertions after failure at low strain velocity: an evaluation of ligament entheses in the rabbit knee.
The morphology of failed rabbit knee entheses is described after in vitro load to failure testing at low strain velocity. Avulsion fracture was the dominant failure mode both for the cruciate ligaments and the medial collateral ligament. The patellar ligament became avulsed in most cases from the patellar insertion. The ligamentous anterior attachment of the medial meniscus failed by a midsubstance rupture and the posterior fibrocartilaginous attachment by a rupture near to the meniscal horn. On histological inspection the failure characteristics usually appeared more elaborate, involving to different degrees all portions of the bone-ligament-bone complexes. Avulsion fracture through subchondral bone was often combined with a partial ligament midsubstance rupture. In few cases avulsion from the cement line was combined with a rupture between the uncalcified fibrocartilage and the ligament. The medial collateral ligament failed in one case entirely at the cement line of its femoral insertion. Horizontal rupture through the calcified fibrocartilage and a vertical cleavage crossing the tidemark were also observed. The superficial portion of the patellar ligament failed with a midsubstance rupture, and the deeper part with an avulsion through the calcified fibrocartilage or an avulsion fracture. The complex failure characteristics may be attributed to uneven loading, nonuniformity of the structure, and specific anatomical location. Subchondral bone beneath femoral and tibial insertions seems to be weaker than the transitional zone between soft tissue and hard bone at the enthesis. The overall inferior structural quality of a ruptured ligament has to be taken into account when parts of the original structure are used for suturing or reconstruction.
Animals↗
Fluoride uptake into the developing enamel and dentine of sheep incisors following daily ingestion of fluoridated milk or water.
Incomplete availability of fluoride from fluoride compounds when added to milk is claimed to reduce the effectiveness of fluoridated milk in caries prevention in humans. This study attempts to add to the understanding of the systemic bioavailability of fluoride ingested with milk compared with water, by measuring its uptake into developing incisors of sheep. Twenty-five sheep, aged approximately 10 months, were randomised into five groups and farmed under identical conditions. They were dosed orally each day for 22 weeks with fluoride as sodium fluoride in water or bovine milk. The doses of fluoride were 0.5 or 0.2 mg/kg body weight in milk or water; a control group received no additional fluoride. Tooth length was labelled at the start of dosing with intramuscular injection of tetracycline. After sacrificing, incisors were removed, sectioned, and analysed for fluoride and calcium by proton microprobe scans from the enamel surface to the pulp. Concentrations of ionised fluoride in the milk samples were 30 percent and 20 percent respectively for added fluoride at concentrations of 300 and 750 micrograms/ml. The mean fluoride concentrations in surface enamel, deep enamel, and dentine were dependent on the daily fluoride dose and independent of the carrier (milk or water). The teeth of sheep receiving the higher fluoride dose (0.5 mg/kg body weight) had significantly higher fluoride than those receiving the lower fluoride dose (0.2 mg/kg body weight), which were significantly higher than in the teeth of the control sheep. It was concluded that the bioavailability of fluoride from sodium fluoride mixed with milk or water was similar following metabolism, despite 20-30 percent and 100 percent ionisation in milk and water respectively.
Animals↗