Biomedical subjects

J G Tully

Publications and source records attributed to J G Tully.

128 records · Page 8Linked to original sources

PubMedDate not supplied

Evaluation of culture media for the recovery of Mycoplasma hominis from the human urogenital tract.

Specimens from the cervix or posterior vaginal fornix of 50 women attending a venereal disease clinic and from the urethra of 50 men with nongonococcal urethritis were cultured for Mycoplasma hominis. Recovery of the organism in two conventional mycoplasma culture media containing horse serum was compared with that in SP-4 broth medium (which is used for the culture of fastidious mycoplasmas). M. hominis was isolated from approximately 63% of the specimens from women by means of either of the horse serum media and from 94% by means of SP-4 medium. The organism was isolated from 10-12% of the urethral samples from men by means of either of the horse serum media and from 22% by means of SP-4 medium. In all, 55 specimens yielded M. hominis in SP-4 medium and 37 in horse serum broth. Thus, 18 (33%) of the 55 isolates were recovered only when SP-4 medium was used. The presence of thallium acetate in the horse serum medium used in one laboratory appeared to contribute to its inferiority. These results show that SP-4 medium may be superior for the isolation of M. hominis in some circumstances. However, major modifications in this medium will be required for the selective recovery of Mycoplasma genitalium from the human urogenital tract when M. hominis is also present.

Animals↗

PubMedDate not supplied

Serological classification of spiroplasmas: current status.

Data concerning serological classification of spiroplasmas are in good agreement, but slightly different numerical designations have been given to existing groups. It is proposed that a standardized system be adopted based on information developed mainly by the IRPCM working team on spiroplasmas. The type species (Spiroplasma citri) should be redefined to include only the agent of citrus stubborn disease (subgroup I-1). Six other subgroups, including three proposed by Bové et al. in this volume (I-5, I-6, and I-7), are members of the Group I complex. Because subgroups I-1, I-2, and I-3 (1) show significant reciprocal differences in DNA-DNA homology and two-dimensional electrophoretic protein profiles, (2) occupy exclusive habitats, (3) are each associated with important diseases, and (4) consist of clusters of very similar or identical strains, it is suggested that Latin binomials could be assigned to subgroups I-2 and I-3. It is proposed that those criteria could serve as general guidelines for consideration of subgroups for species status in the class Mollicutes. The I-4 subgroup is assigned an uncertain status, pending comparisons with the LB-12 (I-5), M55 (I-6), and N525 (I-7) subgroups. To previously described serogroups we add the CN-5 Cotinus beetle spiroplasma (IX), the AES-1 mosquito strain (X), and the MQ-4 Monobia strain (XI).

DNA, Bacterial↗