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Biomedical subjects

J G Kapsenberg

Publications and source records attributed to J G Kapsenberg.

At least 37 records · Page 2Linked to original sources
PubMed1985

Persistent Epstein-Barr virus infection and a histiocytic sarcoma in a renal transplant recipient.

Lymphomas occurring in renal transplant recipients are mostly large cell non-Hodgkin's lymphomas (B-cell-derived). A sarcoma with all morphologic, immunohistochemical, and ultrastructural characteristics of a tumor of the mononuclear phagocytic system (MPS) developed in a 23-year-old woman 1 year after renal transplantation. Anti-Epstein-Barr-virus antibody titers proved to be exceptionally high, even in pretransplant sera. Tumor-derived cells proved to be positive for Epstein-Barr nuclear antigen (EBNA), and hybridization showed multiple copies of Epstein-Barr virus (EBV)-DNA, suggesting a relationship between this tumor and EBV. More widespread use of immunochemical and histochemical diagnostic techniques might detect more cases, which, until now, have probably been diagnosed as B-cell-derived immunoblastic lymphomas.

Adult↗
PubMed1985

Heterogeneity of immune defects in three children with a chronic active Epstein-Barr virus infection.

Three children, all girls, showed long-lasting clinical and serologic evidence of chronic active Epstein-Barr virus (EBV) infection. Extremely high serum titers of IgG- and IgA-type VCA antibodies and EA antibodies were present, whereas EBNA antibody titers were in the range of those found in seropositive individuals. All three patients repeatedly showed the presence of nonspecific pokeweed mitogen (PWM)-activatable suppressor cells in the peripheral blood. The analysis of EBV-specific cytotoxic T cells showed that one patient exhibited normal cytotoxicity, whereas a second patient demonstrated no EBV-specific cytotoxicity together with unusually high levels of virus-infected B cells in the blood and lymph node. The third patient repeatedly showed refractoriness of the circulating B cells to EBV infection, probably on the basis of some developmental defect. It was concluded that each patient has his or her own peculiar defect in the virus-host balance, indicating that heterogeneity may underlie the syndrome of chronic active EBV infection in humans.

Adolescent↗
PubMed1985

Measles virus matrix protein detected by immune fluorescence with monoclonal antibodies in the brain of patients with subacute sclerosing panencephalitis.

Brain materials from four cases of subacute sclerosing panencephalitis were examined by immune fluorescence with monoclonal antibodies against five structural components of measles virus. All five antigens including the matrix component were present in the brain tissues of all cases. A defective Vero cell-associated virus isolate from one of the cases produced all of the structural components except the matrix protein.

Antibodies, Monoclonal↗
PubMed1984

Removal of enteric viruses from surface water at eight waterworks in The Netherlands.

Eight waterworks in The Netherlands, which use surface water as their raw water source, were sampled repeatedly between November 1978 and June 1981. At five waterworks , 30 of 45 samples of raw water contained viruses. Of 55 samples of partially purified water, 11 were virus positive, including 8 after coagulation, sedimentation, and rapid sand filtration, 2 after storage, coagulation, sedimentation, transport chlorination, and rapid sand filtration, and 1 after storage in open reservoirs for 5 months. No viruses were detected in 100 samples of drinking water of 500 liters each from six waterworks . Most isolated viruses were typed, and a great variety of human enteroviruses were found, reflecting both pollution of raw water sources with sewage and vaccination with oral polio vaccine in neighboring countries.

Enterovirus↗
PubMed1984

Adenovirus 38: a new human adenovirus species of subgenus D.

From the stool of a Dutch girl, a new virus (LJ) was isolated that by morphological, biological, and immunological criteria was a human adenovirus. Because of its hemagglutinating activity for rat and human red blood cells and its DNA restriction enzyme pattern, the virus was classified as a member of subgenus D. It was distinct in neutralization tests from all other human adenoviruses. The hemagglutinin was antigenically similar to that of adenoviruses 13 and 39, but the DNA differed from that of these two species. It is proposed to call virus LJ candidate Mastadenovirus h 38. Two other viruses belonging to the same new species were isolated in the USA.

Adenoviruses, Human↗
PubMed1983

Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool.

About 200 antigenically related adenoviruses were isolated from cases of infantile diarrhoea in the Netherlands and North-West Germany. The viruses were fastidious and failed to replicate serially in human diploid fibroblasts and in primary human embryonic kidney cells. A number of strains were established in HeLa, HEp-2, Graham (293), cynomolgus monkey kidney, and Chang conjunctival cells. The viruses were mammalian adenoviruses by the usual criteria. No relationship to the 39 known human adenovirus species was found, either by neutralization tests or by haemagglutination inhibition tests. Neutralization tests showed two distinct variants, represented by strains Tak and Dugan. The variants were identical in haemagglutination inhibition tests. DNA restriction enzyme analysis showed Tak and Dugan to have considerably different genomes, indicating that these variants should be classified as different species (Wadell et al, 1983). It is proposed that the variants should be called Mastadenovirus h 40 (with reference strains Dugan and Hovi X) and Mastadenovirus h 41 (with reference strain Tak). Neutralization and haemagglutination inhibition tests demonstrated that the viruses from Glasgow and Helsinki (Hovi X) described by Johansson et al [1980] and by Kidd and Madeley [1981] belong to these two adenovirus species.

Adenoviridae Infections↗
PubMed1982

Laboratory investigation of two "whitepox" viruses and comparison with two variola strains from southern India.

Two variola-like viruses were isolated in Bilthoven in 1964 from monkey kidney tissue cultures. These viruses, coded 64/7255 and 64/7275, have been considered as two of the six "whitepox" viruses isolated from animal tissues, all of which are indistinguishable from variola virus by laboratory tests.Two specimens from suspect smallpox cases in India were examined in the Bilthoven laboratory at about the same time as the "whitepox" viruses and two strains of variola virus were isolated from them. A detailed comparison of certain biological markers of these four viruses, and of their DNAs, shows that the two "whitepox" viruses could not be distinguished from each other or from one of the two variola isolates. In view of this, and since there was a possibility of cross-contamination at the time of isolation, it is concluded that 64/7255 and 64/7275 must be regarded as genuine variola viruses and be deleted from the list of variola-like viruses isolated from animal tissues.

DNA Restriction Enzymes↗
PubMed1981

Epidemiological implications of the isolations and intratypic serodifferentiation of poliovirus strains in the Netherlands.

From the results of virus isolation and serological studies it can be concluded that poliovirus does not occur endemically in the Netherlands. On the other hand continual importation of poliovirus is demonstrated among children of foreign workers, refugees and adoptive children. A certain part of these imported poliovirus strains appear to be wild type as shown by intratypic serodifferentiation with monospecific antisera. These strains are a continuous danger to non-immunized persons lacking neutralizing antibodies. The intratypic serodifferentiation with monospecific antisera also appears to be applicable for the differentiation of wild poliovirus strains and may therefore be a useful tool in epidemiological studies.

Adolescent↗