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Biomedical subjects

J G Hansen

Publications and source records attributed to J G Hansen.

At least 37 records · Page 2Linked to original sources
PubMed1991

[Measurement of the middle ear pressure in preventive examinations of children].

Tympanometry was performed in 247 children at the prophylactic examinations. The children were aged from 15 months to five years and were included consecutively and examined only once. In 9% it did not prove possible to perform satisfactory tympanograms. 37% had initially pathological tympanometric findings. The frequency of pathological tympanometric findings was found to decrease with increasing age. Pathological tympanometric findings were found to be significantly more frequent in boys. Twenty-two children (8% of the material) were tubulated. It is considered that tympanometry is a useful method for employment in general practice but that further investigations from general practice are still required.

Acoustic Impedance Tests↗
PubMed1991

Treatment of bacterial vaginosis with metronidazole or pivampicillin.

In a double-blind randomized controlled trial from general practice, we assessed the efficacy of treatment with either metronidazole or pivampicillin in patients fulfilling the diagnostic criteria of bacterial vaginosis. 50 women were treated with metronidazole 500 mg twice daily and 54 with pivampicillin 700 mg twice daily, both for seven days. Evaluated four weeks after the start of treatment, we found a significantly higher cure rate in the metronidazole group (90%) than in the pivampicillin group (69%) (p = 0.01). Cure was defined as lack of fulfillment of the diagnostic criteria of bacterial vaginosis. Test for confounding according to parity and complaint of vaginal discharge showed no influence. Side-effects were significantly commoner in the pivampicillin group (28%) than in the metronidazole group (8%). We consider that metronidazole is a potent drug in the treatment of bacterial vaginosis, with pivampicillin as a useful alternative.

Adolescent↗
PubMed1991

Obstructive ventilatory impairment. An investigation in general practice.

We estimated the prevalence of obstructive ventilatory impairment and its relationship to sex, age, chronic bronchitis, asthma, and smoking in a cross-sectional epidemiological study. The population consisted of 3119 persons greater than or equal to 16 years listed as patients in our general practice. From this group, 2740 (88%) underwent dynamic spirometry (Vitalograph). The main results showed a high prevalence of obstruction. 26% had MMEF values less than 75%, 8% FEV-1 less than 75% and 5% FEV-1/FVC less than 65%. Obstruction was significantly correlated with old age, chronic bronchitis, asthma, and smoking.

Adult↗
PubMed1990

[Breaking the smoking habit in general practice].

A total of 1,022 adult smokers were questioned about smoking habits and were submitted to pulmonary function tests with spirometry. These patients were subdivided into two groups depending on whether investigation of pulmonary function showed obstruction or normal findings. All of the patients were given the pamphlet: "Important information about smoking" published by the Danish Anticancer League and they were given standardized warnings about smoking and information about the result of the pulmonary function tests. Twelve months later, the patients were questioned about their current smoking habits. 9% with obstruction and 11% without obstruction, respectively, had stopped smoking.

Adolescent↗
PubMed1989

[Rapid diagnosis of acute tonsillitis caused by streptococcus group A].

In general practice, a material of 202 patients seeking advice on account of difficulty in swallowing were included consecutively in an investigation with the object of assessing the value of a rapid test, Respiralex, for the diagnosis of group A streptococci as compared with culture. The true positive and negative diagnostic frequencies were 0.89 and 0.94, respectively. Absence of agreement was found most frequently when culture rendered poor growth. It is concluded that Respiralex is such a reliable method of examination in the diagnosis of group A streptococci, that it is recommended for use in general practice and that culture is only necessary in patients with negative Respiralex tests and pronounced objective signs of streptococcal tonsillitis.

Acute Disease↗
PubMed1986

Microbiology of vaginal discharge in general practice.

Three groups of women were examined by culture for Gardnerella vaginalis and Candida. Group I consisted of 427 women, who complained spontaneously of vaginal discharge, group II of 311 women who did not complain of vaginal discharge until questioned prior to gynaecological examination, and group III of 100 women who denied vaginal discharge. Groups I and II also had cultures made for Trichomonas vaginalis and Neisseria gonorrhoica. In group I with spontaneous complaints the one-year prevalence rate of vaginal discharge was 3.4%. Candida, Trichomonas vaginalis and Neisseria gonorrhoica were cultured in 24%, 8% and 1% respectively. Gardnerella vaginalis was cultured in half of the women in all groups. A characteristic discharge or a positive microscopic finding was related to high concentration of Gardnerella vaginalis. A characteristic discharge even without spontaneous complaints of vaginal discharge was related to a high prevalence of Gardnerella vaginalis. Women complaining from discharge had higher concentrations of Gardnerella vaginalis than non-complaining. This leads to the conclusion that clinical disease may not be present unless concentrations of Gardnerella vaginalis have risen above some minimum level. The Gardnerella vaginalis syndrome defined by positive culture, clinical finding and microscopy was found in 20% of symptomatic women.

Adolescent↗
PubMed1985

Vaginal discharge and Gardnerella vaginalis. Predisposing factors.

A total of 284 women in the reproductive years with vaginal discharge from which only Gardnerella vaginalis (GV) had been isolated, entered a questionnaire study on the possible causal relation between presence of GV and type of menstrual hygiene, type of contraception, number of deliveries, infertility and number of sexual partners. A control group consisted of 225 women without vaginal discharge. The study showed that patients with GV significantly more frequently used tampons for menstrual hygiene, sought medical assistance for vaginal discharge more often, and had more sexual partners. On the other hand, no difference was found between the two groups as to type of contraception, number of previous deliveries, and unsuccessful attempts of getting pregnant.

Female↗
PubMed1985

Diagnostic value of clinical examination, direct microscopy, and culture in the Gardnerella vaginalis syndrome.

In a general practice 467 women aged 15-49 years with vaginal discharge were consecutively examined in order to compare the diagnostic value in the Gardnerella vaginalis syndrome of the clinical examination, direct microscopy of the vaginal secretion, and culture. We found significant correlations between all relevant combinations of the diagnostic methods. The predictive value of a positive test for the clinical examination, the microscopy, and the clinical examination combined with microscopy, respectively, was 0.75, 0.89 and 0.90, whereas the predictive values of the negative test were 0.59, 0.72 and 0.61, respectively. Because even small numbers of Gardnerella vaginalis may yield growth on selective culture media, the clinical examination and the microscopy are often negative despite positive culture. To avoid overtreatment of a benign condition we therefore conclude that the presence of a characteristic clinical picture and positive microscopy constitute a safe basis for the diagnosis of the Gardnerella vaginalis syndrome, and that the diagnosis should be based on this combination or on the fulfilment of three of the following four criteria: 1) characteristic vaginal secretion; 2) vaginal pH greater than 4.5; 3) positive potassium hydroxide test or characteristic herring-brine smell, and 4) clue cells at microscopy. With the present methods culture for Gardnerella vaginalis should not routinely be performed, until the value of quantitated methods has been proved. Coexistence of other microorganisms rendered the diagnosis difficult. We recommend to treat the most predominant infection first.

Adolescent↗
PubMed1985

Treatment of the Gardnerella vaginalis syndrome. A controlled, double-blind study comparing pivampicillin and metronidazole.

A double-blind, randomized, therapeutic study was carried out in 289 patients with vaginal discharge and growth of Gardnerella vaginalis (GV) and no growth of Neisseria gonorrhoeae, Trichomonas vaginalis or Candida. Treatment consisted of either pivampicillin (Pondocillin), 700 mg twice daily for seven days, or metronidazole, 500 mg twice daily for seven days. The effect was evaluated on the basis of the patient's statement and on the result of culture for GV immediately after end of treatment. Evaluated from the culture results metronidazole was significantly more effective than pivampicillin in eradicating GV. The efficacy of the two medications was 69% and 54%, respectively. The evaluations by the patients in the two groups, on the other hand, did not differ significantly, although there was a tendency to consider metronidazole as the most effective. Metronidazole must be considered the most potent drug today, and we recommend it as the medication of choice, provided that the diagnosis is confirmed by the clinical picture and microscopy and not only by culture.

Ampicillin↗
PubMed1983

Monoclonal antibody to human 66,000 molecular weight plasminogen activator from melanoma cells. Specific enzyme inhibition and one-step affinity purification.

Hybridomas producing a monoclonal IgG1 antibody to a human plasminogen-activating enzyme with an apparent mol. wt. of 66,000 (66 K, HPA66) from human melanoma cells were obtained by fusion of NSI-Ag 4/1 mouse myeloma cells with spleen cells from a mouse immunized with a partially purified preparation of the enzyme. Screening for clones of hybridomas producing antibodies to HPA66 was performed with the impure enzyme preparation. A preliminary screening included enzyme-linked immunosorbent assay and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting; the final identification was based on inhibition of the enzymatic activity of HPA66 which was complete at high antibody concentrations. No inhibition of three other human and murine plasminogen activators or of plasmin was observed. Employing a one-step affinity procedure with the antibody coupled to Sepharose, HPA66 was purified approximately 200-fold from conditioned medium from the melanoma cells with a yield of 79%. The purified HPA66 was homogeneous as evaluated by SDS-PAGE. Electrophoresis under reducing conditions indicated that it consisted of one polypeptide chain. The binding constant between the antibody and 125I-labelled HPA66 was approximately 2.5 x 10(9) l/mol. The antibody did not bind to a variety of other plasminogen activators, including 52-K and 36-K human enzymes and 48-K and 75-K murine enzymes. Previously, a monoclonal antibody against another enzyme was derived by the sole use of enzyme inhibition for screening. The present study represents a modification of this procedure that can be used when antibody-unrelated inhibitors of the enzyme are present in hybridoma culture fluid.

Animals↗