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J Fukushima

Publications and source records attributed to J Fukushima.

At least 109 records · Page 6Linked to original sources
PubMed1991

Possible downward burster-driving neurons related to the anterior semicircular canal in the region of the interstitial nucleus of Cajal in alert cats.

It is well known that vestibular nystagmus evoked by head rotation occurs in the plane specific to that in which head rotation was applied in three-dimensional space. Although burster-driving neurons (BDN) have been demonstrated for a quick phase of horizontal nystagmus, it is not yet known where the counterpart for vertical nystagmus is located. We analyzed the activity of a class of neurons in the region within, and in the close vicinity of, the interstitial nucleus of Cajal (INC) in alert cats. Their activity gradually increased during an upward slow phase evoked by nose-down pitch. This increased activity was further followed by burst discharge shortly before and during the downward quick phase. Gradually increased activity was also evoked by contralateral roll. These results suggest that the gradually increased activity was evoked by activation of the contralateral anterior canal. Many of these cells were fired by electrical stimulation of the contralateral vestibular nerve with short latencies. These cells also showed burst discharge shortly before and during downward saccades induced by visual stimuli, and the number of spikes during bursts was correlated with saccade amplitudes. Although all had irregular resting discharges, eye-position-related activity was rarely obtained. The characteristic behavior of these cells is very similar, except for their on-directions, to the behavior of horizontal BDNs, suggesting that these INC cells are a candidate for downward BDNs related to the anterior canal.

Animals↗
PubMed1991

The pituitary-specific regulatory gene GHF1 contains a minimal cell type-specific promoter centered around its TATA box.

GHF-1 is a pituitary-specific transcription factor responsible for activation of the growth hormone (GH) gene. The GHF1 gene is expressed exclusively in cells of the somatotrophic lineage, and its transcription is extinguished in somatic cell hybrids. The minimal sequences required for differential transcription of GHF1 in GH-expressing and -nonexpressing cell lines and somatic cell hybrids were localized to a 15-bp region surrounding and including its TATA box. This 15-bp fragment acts as a cell type-specific promoter element and is recognized by a transcription factor present in GH-expressing cell lines. Hence, in addition to enhancers and upstream promoter elements, the TATA element (TATA box plus surrounding sequences) can be, in certain cases, an important determinant of cell-type-specific transcription.

Animals↗
PubMed1991

Detection of elastase production in Escherichia coli with the elastase structural gene from several non-elastase-producing strains of Pseudomonas aeruginosa.

The elastase structural gene from Pseudomonas aeruginosa IFO 3455 has been cloned and sequenced. Using this gene as a probe, we cloned the DNA fragments (pEL3080R, pEL10, and pEL103R) of the elastase gene from non-elastase-producing strains (P. aeruginosa IFO 3080, N-10, and PA103 respectively). These three Pseudomonas strains showed no detectable levels of elastase antigenicity by Western blotting (immunoblotting) or by elastase activity. When elastase structural genes about 8 kb in length were cloned into pUC18, an Escherichia coli expression vector, we were able to detect both elastase antigenicity and elastolytic activity in two bacterial clones (E. coli pEL10 and E. coli pEL103R). However, neither elastolytic activity nor elastase antigenicity was detected in the E. coli pEL3080R clone, although elastase mRNA was observed. The partial restriction map determined with several restriction enzymes of these three structural genes corresponded to that of P. aeruginosa IFO 3455. We sequenced the three DNA segments of the elastase gene from non-elastase-producing strains and compared the sequences with those from the elastase-producing P. aeruginosa strains IFO 3455 and PAO1. In P. aeruginosa N-10 and PA103, the sequences were almost identical to those from elastase-producing strains, except for several nucleotide differences. These minor differences may reflect a microheterogeneity of the elastase gene. These results suggest that two of the non-elastase-producing strains have the normal elastase structural gene and that elastase production is repressed by regulation of this gene expression in P. aeruginosa. Possible reasons for the lack of expression in these two strains are offered in this paper. In P. aeruginosa IFO 3080, the sequence had a 1-base deletion in the coding region, which should have caused a frameshift variation in the amino acid sequence. At present, we have no explanation for the abnormal posttransciptional behavior of this strain.

Amino Acid Sequence↗
PubMed1991

Eye movement and neuronal response in the region of the interstitial nucleus of Cajal during sinusoidal vertical linear acceleration in alert cats.

We examined neuronal behavior and eye movement response to sinusoidal vertical linear acceleration in the INC region. In light, robust eye movement response with near compensatory phase was consistently evoked over wide frequencies. Without visual inputs, the linear vestibulo-occular reflex (LVOR) with advanced phase was inconsistently evoked. Optokinetic stimulation alone produced more lag of response phase as stimulus frequency increased, suggesting that the otolith inputs contributed to the eye movement response with near compensatory phase in light. The activity of all burst-tonic (BT) neurons tested which showed close correlation with spontaneous vertical eye movement was modulated during linear motion in light and during optokinetic stimulation in association with eye movement responses. Without visual inputs, similar linear motion produced no response in a majority of BT cells even when the LVOR was evoked. These results suggest that BT cells are involved in some aspect of the generation of a functionally important vertical eye position signal using the otolith and visual inputs. Many cells in the INC region that did not belong to BT cells responded to linear acceleration without visual inputs and optokinetic stimulation even when eye movement response was not evoked, indicating that these cells received the otolith and visual inputs.

Acceleration↗
PubMed1990

Further analysis of the control of voluntary saccadic eye movements in schizophrenic patients.

Many schizophrenic patients reveal abnormalities in the antisaccade task. To better understand the nature of these abnormalities, in the present study we have assigned to schizophrenics the no-saccade task (subjects were required to remain fixated without being disturbed by a reflexive saccade) and memory-saccade task (subjects were required to look at a remembered target) in addition to the antisaccade and saccade tasks used previously. Many schizophrenics revealed higher error rates in the no-saccade task, and latencies of saccades to a memorized target were significantly longer than controls in the memory-saccade task. Peak velocities of saccades of large amplitudes in the memory-saccade and antisaccade tasks (but not in the saccade task) were significantly slower and durations of such saccades were longer than normal controls despite the similarity between the distributions of amplitudes of such saccades between the patients and controls. These results suggest that many schizophrenics have difficulty suppressing reflexive saccades and initiating and executing appropriate volitional saccades when the goal for the movements is known but not visible.

Adolescent↗
PubMed1990

Spatial properties of vertical eye movement-related neurons in the region of the interstitial nucleus of Cajal in awake cats.

1. Maximal activation directions of vertical burst-tonic and tonic neurons in the region of the interstitial nucleus of Cajal (INC) were examined in alert cats during vertical vestibulo-ocular reflex induced by sinusoidal rotation (at 0.11 Hz +/- 10 deg, or 0.31 Hz +/- 5 deg) in a variety of vertical planes using a null point analysis. The results were compared with the angles of anatomical and functional planes of vertical canals reported by Blanks et al. (1972) and Robinson (1982), and with the angles of vertical eye muscles measured in this study and by Ezure and Graf (1984). 2. Maximal activation directions of 23 cells (21 burst-tonic and 2 tonic neurons) were determined from their responses during rotation in 4 or more different vertical planes. All cells showed sinusoidal gain curves and virtually constant phase values except near the null regions, suggesting that their responses were evoked primarily by canal inputs. Phase values of 5 cells near the null regions depended on the rotation plane, suggesting additional otolith inputs. We used a measurement error range of +/- 10 deg for calculating the maximal activation directions from the null regions of individual cells and the values of error ranges of null calculation. Of the 23, the maximal activation directions of 7 cells were outside the measurement error ranges of vertical eye muscle angles and within the ranges of vertical canal angles (class A), those of 5 cells were within the ranges of eye muscle angles and outside the ranges of vertical canal angles (class B), and those of the remaining 11 cells were in the overlapping ranges for both angles (class C). Even if only the cells in which 5 or more measurement points were taken to determine maximal activation directions (n = 15), the results were similar. During vertical rotation with the head orientation +60 deg off the pitch plane, dissociation of cell activity and vertical compensatory eye movement was observed in 5 cells in class A or C that had null angles near +45 deg. These results suggest that the cells in class A and B carried individual vertical canal and oculomotor signals, respectively, although it is difficult to tell for the majority of cells (class C) which signals they reflected. Some cells in class A and C were antidromically activated from the medial longitudinal fasciculus at the level of abducens nucleus, suggesting that the signals carried by these cells may be sent to the lower brainstem.(ABSTRACT TRUNCATED AT 400 WORDS)

Action Potentials↗
PubMed1990

Neuronal activity related to vertical eye movement in the region of the interstitial nucleus of Cajal in alert cats.

(1) Discharge characteristics of neurons in the region of the interstitial nucleus of Cajal (INC) were studied in alert cats during spontaneous or visually induced eye movement and sinusoidal vertical (pitch) rotation. Activity of a majority of cells (n = 68) was closely related to vertical eye position with or without bursting activity during on-direction saccades. They were called vertical burst-tonic (n = 62) and tonic (n = 6) neurons. Mean discharge rates for individual cells when the eye was near the primary position ranged from 35 to 133 (mean 75) spikes/s with a coefficient of variation (CV) ranging from 0.04 to 0.29 (mean 0.15). Average rate position curves were linear for the great majority of these cells with a mean slope of 3.9 +/- 1.2 SD spikes/s/deg. (2) The burst index was defined as the difference in discharge rate between maximal rate during an on-direction saccade and the tonic rate after the saccade. The values of mean burst index for individual cells ranged from 8 to 352 (mean 135) spikes/s. Tonic neurons had a burst index lower than 60 spikes/s and were distributed in the lower end of the continuous histogram, suggesting that burst-tonic and tonic neurons may be a continuous group with varying degrees of burst components. During off-direction saccades, a pause was not always observed, although discharge rate consistently decreased and pauses were seen when saccades were made further in the off-direction toward recruitment thresholds. Significant positive correlation was observed between average discharge rate during off- as well as on-direction saccades and tonic discharge rate after saccades for individual cells, which was not due to cats making saccades mainly from the primary position. (3) During pitch rotation at 0.11 Hz (+/- 10 deg), burst-tonic and tonic neurons had mean phase lag and gain of 128 (+/- 13 SD) deg and 4.2 (+/- 1.7 SD) spikes/s/deg/s2 relative to head acceleration. During pitch rotation of a wide frequency range (0.044-0.495 Hz), the values of phase lag were mostly constant (120-140 deg), while simultaneously recorded vertical VOR showed the mean phase lag of 178 deg. Vertical eye position sensitivity and pitch gain (re head position) showed significant positive correlation.(ABSTRACT TRUNCATED AT 400 WORDS)

Action Potentials↗
PubMed1990

Voluntary control of saccadic eye movements in patients with schizophrenic and affective disorders.

We have examined visually guided saccade and antisaccade tasks in 32 schizophrenics, 13 patients with affective disorders and 36 normal controls. Latencies of saccades were within the normal range in all patients examined. In the antisaccade task, 26/32 schizophrenics showed more errors and/or longer latencies, whereas 11/13 patients with affective disorders showed normal results. One manic patient showed significantly longer latency, and one bipolar patient showed a borderline error rate (15%). These results indicate that the antisaccade abnormalities in schizophrenics were not correlated with age, duration of illness, length of education or quantities of medication. None of the schizophrenics with normal antisaccades showed abnormalities in CT scans, while 73% of the schizophrenics who showed both higher error rate and longer latencies showed atrophy of the frontal cortex. The scores of conceptual disorganization and motor retardation in Brief Psychiatric Rating Scale (BPRS) were significantly higher in schizophrenics with abnormal antisaccades, while acute symptoms such as delusions and hallucinations were not correlated with the abnormalities.

Adolescent↗
PubMed1990

Activity of eye-movement-related neurons in the region of the interstitial nucleus of Cajal during sleep.

Activity of vertical burst-tonic neurons in the region of the interstitial nucleus of Cajal (INC) in cats that showed a close correlation with spontaneous vertical eye movement during the waking state was compared to that during sleep. All the cells tested maintained high and regular discharge rates similar to those during the waking state when the eye was near the primary position. However, a significant correlation between tonic discharge rates and vertical eye position change seen during the waking state was lost during slow drifting eye movement during sleep, indicating that they are not involved in such eye movement. Upward (or downward) burst-tonic neurons showed bursts (or decreased activity) during upward rapid-eye movements (REMs) accompanied by failure of eye position holding with almost exponential decay during REM sleep. However, the increased (or decreased) activity was not maintained and quickly returned to near-previous discharge rates. Despite the fact that a significant positive correlation was seen between average discharge rates during vertical saccades and tonic rates after saccades for these neurons during the waking state, the same cells lost such a correlation during vertical REMs with eye position holding failure. The close correlation between presence or absence of tonic activity related to preceding bursts of burst-tonic neurons, on the one hand, and holding or failure of vertical eye position after vertical saccades or REMs, on the other, suggests that these neurons receive excitatory and inhibitory burst inputs, and also that they are involved in some aspect of vertical eye position generation, but that the INC region alone cannot convert the burst signals into eye position.

Animals↗
PubMed1990

Molecular cloning and partial DNA sequencing of the collagenase gene of Vibrio alginolyticus.

DNA fragments Vibrio alginolyticus chemovar iophagus, at least 7 kb in length, were ligated to Escherichia coli expression vectors. Three clones of Escherichia coli HB101 (pLCO-1, pLCO-2, pLCO-3) were obtained by the colony immunoblotting method using anti-collagenase antibody. In Escherichia coli, all these genes produced collagenase antigens which were detected with Western blotting. The amino acid sequence of chemically purified collagenase fragments was also analyzed. An approximately 2.5 kb DNA fragment of the pLCO-1 clone was sequenced, and we found that portions of the deduced amino acid sequence of the chemically analyzed fragments. Therefore, it is highly probable that the gene studied in the present experiment is truly a collagenase structural gene.

Amino Acid Sequence↗
PubMed1990

Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455.

The DNA-encoding alkaline proteinase (AP) of Pseudomonas aeruginosa IFO 3455 was cloned, and its complete nucleotide sequence was determined. When the cloned gene was ligated to pUC18, the Escherichia coli expression vector, the gene-incorporated bacteria expressed high levels of both AP activity and AP antigens. The amino acid sequence deduced from the nucleotide sequence revealed that the mature AP consists of 467 amino acids with a relative molecular weight of 49,507. The amino acid composition predicted from the DNA sequence was similar to the chemically determined composition of purified AP reported previously. The amino acid sequence analysis revealed that both the N-terminal side sequence of the purified AP and several internal lysyl peptide fragments were identical to the deduced amino acid sequences. The percent homology of amino acid sequences between AP and Serratia protease was about 55%. The zinc ligands and an active site of the AP were predicted by comparing the structure of the enzyme with of Serratia protease, thermolysin, Bacillus subtilis neutral protease, and Pseudomonas elastase.

Amino Acid Sequence↗
PubMed1989

Molecular cloning and nucleotide sequence of rat brain argininosuccinate lyase cDNA with an extremely long 5'-untranslated sequence: evidence for the identity of the brain and liver enzymes.

Argininosuccinate lyase (EC 4.3.2.1) is an enzyme present in the brain of ureotelic animals. Using as a probe rat liver argininosuccinate lyase cDNA, already isolated and sequenced (Amaya, Y., Matsubasa, T., Takiguchi, M., Kobayashi, K., Saheki, T., Kawamoto, S. and Mori, M., J. Biochem., 103 (1988) 177-181), we screened a rat brain cDNA library constructed in the lambda gt11 expression vector and obtained a single cDNA clone. This cDNA clone contained an open reading frame encoding a polypeptide of 461 amino acid residues (predicted Mr = 51,390), a 5'-untranslated sequence of 967 bp and a 3'-untranslated sequence of 74 bp. The length of the 5'-non-coding region of the cDNA seems to be one of the longest among the cDNAs heretofore isolated. A comparison of the brain cDNA sequence (2424 bp) with the corresponding region of the liver cDNA (1574 bp) revealed differences in 5 nucleotides. The brain clone contained A----G and C----G base differences from the hepatic sequence, resulting in amino acid changes from Tyr and Arg in the liver clone, to Cys and Gly in the brain clone, respectively. The other 3 nucleotide differences are silent with respect to the amino acid sequence of the protein. Therefore, the amino acid sequence of the brain argininosuccinate lyase, as deduced from the nucleotide sequence of its cDNA clone, was identical with that of the liver protein, except for two amino acid residues. These minor changes may reflect a microheterogeneity of the argininosuccinate lyase gene. The brain and liver enzymes seem to be encoded by the same structural gene.

Amino Acid Sequence↗
PubMed1989

Intrahepatic bile duct changes in human hepatosplenic schistosomiasis mansoni.

Wedge liver biopsies of 132 patients with hepatosplenic mansonian schistosomiasis were studied and divided in two groups according to the presence (Group I - 69 cases) or absence (Group II - 63 cases) of markers of the actual presence of the parasite in the liver tissue. Histological variables indicating bile duct injury were analysed in each case: periductal fibrosis, hyperplasia of the bile duct epithelium, bile duct degeneration, and marginal ductular proliferation. The presence of one or more of these variables defined two sub-groups: A - bile duct lesions present (73 cases), and B - bile duct lesions absent (59 cases). The variables "bile duct degeneration" and "ductular proliferation" were related to the actual presence of the parasite in the host. In 55.3% of all cases of human mansonian schistosomiasis a spectrum of injuries to the bile ducts was present. Epithelial hyperplasia alone or associated with patterns of mucopolysaccharide production was observed in 87.6% cases of the Sub-group A. The bile ducts changes in mansonian schistosomiasis are close to those described in liver fluke infestations such as clonorchiasis, fascioliasis and opistorchiasis. Statistical analysis revealed that high mucopolysaccharide production was associated with epithelial hyperplasia. The pathogenesis of the bile duct changes in human mansonian schistosomiasis and its relation to the parasitic infestations and their antigens is discussed.

Bile Ducts, Intrahepatic↗
PubMed1989

Structural gene and complete amino acid sequence of Pseudomonas aeruginosa IFO 3455 elastase.

The DNA encoding the elastase of Pseudomonas aeruginosa IFO 3455 was cloned, and its complete nucleotide sequence was determined. When the cloned gene was ligated to pUC18, the Escherichia coli expression vector, bacteria carrying the gene exhibited high levels of both elastase activity and elastase antigens. The amino acid sequence, deduced from the nucleotide sequence, revealed that the mature elastase consisted of 301 amino acids with a relative molecular mass of 32,926 daltons. The amino acid composition predicted from the DNA sequence was quite similar to the chemically determined composition of purified elastase reported previously. We also observed nucleotide sequence encoding a signal peptide and "pro" sequence consisting of 197 amino acids upstream from the mature elastase protein gene. The amino acid sequence analysis revealed that both the N-terminal sequence of the purified elastase and the N-terminal side sequences of the C-terminal tryptic peptide as well as the internal lysyl peptide fragment were completely identical to the deduced amino acid sequences. The pattern of identity of amino acid sequences was quite evident in the regions that include structurally and functionally important residues of Bacillus subtilis thermolysin.

Amino Acid Sequence↗
PubMed1989

Adaptive changes of vertical vestibulo-ocular reflex induced by tenectomy of vertical recti muscles and neuronal behavior related to vertical eye movement in the region of the interstitial nucleus of Cajal in alert cats.

Experiments were performed in alert cats to examine adaptive changes of the vertical vestibulo-ocular reflex (VOR) induced by tenectomy of the vertical recti muscles of one eye and behavior of neurons in the region of the interstitial nucleus of Cajal (INC) during this adaptation. Continuous pitch rotations (0.11 Hz) of a constant amplitude (+/- 10 degrees) with only the operated eye uncovered produced a gradual and significant increase in VOR gain that started within 15 min, reaching a plateau within 1 h. When only the normal eye was allowed to view again, VOR gain quickly decreased to the control values within 10 min. Such reversible gain change was not observed when visual input was not allowed to the operated eye, indicating that these changes are adaptive ones to reduce visual-vestibular conflict. A total of 18 neurons were recorded from the INC region during the VOR adaptation. These include 9 eye-movement-related neurons (8 vertical burst-tonic neurons and 1 vertical tonic neuron) and 9 other neurons that did not show close correlation with eye movement but were activated by pitch rotations (tentatively called pitch cells). During adaptive gain increase of the vertical VOR, all the vertical burst-tonic and tonic neurons showed a significant gain increase, whereas a significant change in response gain was observed in only 1 of the 9 pitch cells, indicating that vertical burst-tonic neurons in the INC region are involved in the adaptive gain increase in the vertical VOR.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗