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Biomedical subjects

J Fukuda

Publications and source records attributed to J Fukuda.

At least 127 records · Page 7Linked to original sources
PubMed1992

Successful treatment of primary gastric non-Hodgkin's lymphoma with chemotherapy and radiotherapy.

A case of primary gastric lymphoma is reported. The lymphoma was of the diffusely infiltrating type extending from the supraangular region to the prepyloric region of the stomach. The patient refused gastrectomy, therefore combined treatment with BACOP chemotherapy and radiotherapy was carried out. Complete remission was achieved without any major complications. The patient has remained free from relapse for over 20 months.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1991

New plastic plates which enhance neurite extension in culture: roles of bisphenol-A and tricyclodecanyl units for growth and orientation of neurites on plastic plates with microstructures.

In order to study molecular mechanisms of contact guidance of neurites, dissociated culture of adult mouse dorsal root ganglion (DRG) cells was performed for 6 species of plastic plates different in monomer compositions. Microstructures (grooves of 5-10 microns wide and 1 micron deep) were grafted on the plates so as to guide neurite growth in their axial direction. Neurites were longer and more oriented on plates with larger water contact angle. The distribution of neurites was uneven between grooves and steps of the microstructures, exhibiting a bell-shaped curve in relation to the water contact angle of the plastic. These indicate that hydrophilic properties of the plastic plate surface, due to the presence of bisphenol-A and tricyclodecanyl units, are crucially involved in the elongation and orientation of neurites.

Animals↗
PubMed1991

Age-associated and cell-type specific changes in NGF requirement for neurite regeneration from trigeminal ganglion cells of the shrew (Suncus murinus).

Low density (20-50 cells/cm2), dissociated cultures of trigeminal ganglion (TRG) cells of the shrew, were made in a serum-free medium, and neurite growth was compared across ages of the animal and between NGF-free and NGF-rich conditions. TRG cells from newborn shrew (0-3 days old), which grew long neurites in an NGF-rich medium, failed to grow any neurites in an NGF-free medium. In contrast, TRG cells from aged shrew (16-19 months), which grew neurites without NGF, exhibited no further increment in neurite length when NGF (50-200 ng/ml) was added to the culture medium. TRG cells from adult shrew (4-5 months) were revealed to be a mixture of NGF-dependent and NGF-independent cells. The NGF-dependent cells (20% of the population) had large-sized somata of 24-32 microns diameter (L-type cells) and 2-7 long neurites enriched with arborizations. The remaining NGF-independent cells (80%) had small-sized somata (15-25 microns, S-type cells), and grew 1-3 neurites with a small number of arborizations. These findings suggest that the primary sensory neuron of the shrew has a cell-type specific critical period in the aging process with respect to the requirement of NGF for neurite promotion.

Aging↗
PubMed1990

[Long-term results of valve replacement in the right side of the heart in congenital heart disease--comparative study of bioprosthetic valve and mechanical valve].

Valve replacements in the right side of the heart (TVR and PVR) were done on 16 patients with congenital heart disease, mainly tetralogy of Fallot and Ebstein anomaly. Including reoperations, 19 operations were performed on them and 20 artificial valves were inserted. Ten mechanical valves (7 St. Jude Medical valves, 3 Starr-Edwards valves) and 10 bioprosthetic valves (7 Carpentier-Edwards valves, 3 Ionescu-Shiley valves) were used. Age at valve replacement ranged from 9 to 52 years (mean 23.0 years), and the follow-up period was 1.28-19.8 years (mean 5.7 years). Including 2 sudden deaths, late death occurred in 4 patients, on all of whom mechanical valve replacements were done at the primary operation. Five-year survival rate of all patients was 76.4 +/- 12.1%, and 10-year survival rate was 63.6 +/- 15.4%. All the patients who received bioprosthesis at the primary operation survived at the time of this follow-up study. On the contrary, long-term results of mechanical valve was unsatisfactory with the 5-year survival of 62.5 +/- 17.1%. In spite of anti-coagulation therapy with warfarin, three patients with mechanical valve complicated thrombotic valves, which necessitated re-operations. Calcified bioprosthetic valve occurred in one patient with I-S valve 8.5 years after the implantation. Five-year complication-free rate was 87.5 +/- 11.7% for bioprosthesis, whereas it was 50.0 +/- 15.8% for mechanical valve (p less than 0.056). It is concluded that the bioprosthesis is the first choice for the valve replacement in the right side of the heart in congenital heart disease.

Adolescent↗
PubMed1990

Severe cerebral and systemic necrotizing vasculitis developing during pregnancy in a case of systemic lupus erythematosus.

We describe a fatal case of systemic lupus erythematosus (SLE) developing cerebral and systemic necrotizing vasculitis during pregnancy. The patient was discovered to have SLE at 14 weeks' gestation. Although the symptoms disappeared without treatment with corticosteroid in the 2nd trimester, she presented with meningoencephalitis due to vasculitis in the 3rd trimester. Polyarteritis nodosa (PAN)-like necrotizing vasculitis of the small muscular arteries and arterioles, with acute and healing lesions in the leptomeninges, brain parenchyma and visceral organs was observed at postpartum autopsy. PAN-like vasculitis in the central nervous system is quite rare in SLE. This case is also suggestive in terms of the influence of pregnancy on the activity of SLE.

Adult↗
PubMed1990

[An association of Shy-Drager syndrome with moyamoya disease--a case report].

A 58-year-old female was admitted to our hospital because of orthostatic syncope, and difficulty in urination and walking for 2 years. At age 35, she suffered from cerebral hemorrhage and was diagnosed as Moyamoya disease (MD). Neurological examination on admission revealed severe orthostatic syncope so that she could not stand. At that time, her blood pressure and heart rate were 104/74 mmHg and 78/min in supine position and 52/48 mmHg and 52/min in 60 degrees head-up position. She also had severe pollakisuria, cerebellar ataxia and dysarthria. Cranial nerves, motor strength and deep reflexes were normal. ECG, chest X-ray, and EEG were normal. Aschner, Czermak, and cold pressor tests revealed no response. Urodynamic study revealed autonomic bladder. MRI showed only enlarged fourth ventricle because of atrophy of the pons. Bilateral CAG revealed "Moyamoya" vessels in the cerebral basal regions. Cerebral blood flow (CBF) was measured by 133Xe inhalation method. Mean arterial blood pressure changed from 134 mmHg to 126 mmHg in 45 degrees head-up tilting and CBF decreased from 47.5 mg/100 g/min to 37 ml/100 g/min position. Though there was no relationship between SDS and MD, each one shows dysautoregulation of CBF, it is supposed that a severe orthostatic syncope attack was resulted from synergism of both effects.

Arterial Occlusive Diseases↗
PubMed1989

Hair aluminium in normal aged and senile dementia of Alzheimer type.

To investigate relationship between aluminium and senile dementia of Alzheimer type (SDAT), hair aluminium concentration was measured by ICP method in 35 cases of SDAT (mean 80 yr.) diagnosed clinically and 71 normal aged volunteers (mean 76 yr.). In normal aged, cerebral blood flow (CBF) also determined by Xe133 inhalation method simultaneously. Mean hair aluminium concentration in SDAT was within normal range of Japanese subjects and significantly lower than that of normal aged controls. There was no significant aging effect to hair aluminium concentration in both groups. Hair calcium and magnesium concentrations were significantly lower in SDAT than that of normal aged. Hair aluminium positively correlated to hair calcium and magnesium in SDAT. Hair aluminium negatively correlated to CBF, and CBF positively correlated to calcium and magnesium concentrations in hair in normal aged. Hair aluminium concentration does not appear to be of use in the diagnosis of SDAT. However, it is undeniable that decreased calcium and magnesium enhance selective accumulation of aluminium in the brain in SDAT. Inverse correlation between hair aluminium and CBF in normal aged suggests that aluminium might have some contribution to aging of the brain.

Affect↗
PubMed1989

[Scimitar syndrome with left pulmonary hypertension and right pulmonary artery stenosis in childhood--report of a case of successful surgical repair].

An one-year old child with Scimitar syndrome (anomalous drainage of the right pulmonary vein to inferior vena cava with right lung hypoplasia) associated with left side pulmonary hypertension and right pulmonary artery stenosis underwent surgical repair. The anomalous pulmonary vein was anastomosed to right atrium with 10 mm PTFE graft interposition and drained to left atrium through equine pericardial intra-atrial baffle. The stenotic portion of right pulmonary artery was enlarged with porcine pericardial patch. To our knowledge, this is the first successful surgical case of Scimitar syndrome with pulmonary hypertension in childhood reported in literature. A repair of anomalous pulmonary venous drainage to inferior vena cava in childhood is difficult, and a meticulous selection of surgical procedure is necessary.

Blood Vessel Prosthesis↗
PubMed1989

[Myocardial infarctectomy in the acute stage].

Seven patients with ventricular septal perforation or left ventricular free-wall rupture underwent myocardial resection in the acute stage of myocardial infarction. One of them received direct closure of the left ventricle, and the others replacement of myocardium with a Dacron prosthesis. One patient died of cardiac failure 5 days after surgery, and 4 in the late-stage between 2 months and 4 years. The following conclusions are obtained in our patients: 1. Echocardiography is very important for the diagnosis of cardiac free-wall rupture and determination of emergency surgery. 2. An intra-aortic balloon should be inserted in patients with VSP as soon as possible. If general state of the patients had been improved by the procedure, surgery might be withheld until the recovery, otherwise emergency surgery should be considered. 3. Myocardial replacement with a prosthesis seems to be useful when the left ventricular cavity is predicted to be small after resection of the myocardium. 4. The development of assisted circulation would improve a survival rate after the surgery. 5. Systemic managements are also important because the patients sometimes become fatal by non-cardiac complications even if they have survived from circulatory failure.

Aged↗
PubMed1988

Inhibition by K-252a, a new inhibitor of protein kinase, of nerve growth factor-induced neurite outgrowth of chick embryo dorsal root ganglion cells.

The effect of K-252a, a new alkaloid-like kinase inhibitor from the culture broth of Nocardiopsis sp., on the action of nerve growth factor on normal nerve cells was examined. Nerve cells were isolated from the dorsal root ganglia of chick embryo by trituration without using digesting enzymes, and were seeded on collagen- or poly-L-lysine-coated plastic dishes containing serum-free medium. K-252a at 50-100 nM reversibly inhibited the formation of neurites by dorsal root ganglion cells elicited by the treatment with nerve growth factor.

Animals↗
PubMed1988

Recognition of artificial microstructures by sensory nerve fibers in culture.

Dissociated culture of adult mouse dorsal root ganglion cells on glass plates, on which grating-associated microstructures (a repetition of microgrooves [mGRV] and microsteps [mSTP] of 0.1-10 micron) are fabricated by the conventional lithographic techniques, represents a remarkable bi-directional growth of their nerve fibers in the axial direction of the grating. Microscopical observation shows that the nerve fibers prefer to grow in the mGRV (70%), while their growth cones exhibit an even distribution onto the mGRV and mSTP. The efficiency of the nerve fibers to grow along the grating-axis are highly sensitive to a fine alteration of the width and depth of the mGRV. The preferential growth of the nerve fibers is thus due to a mechanical recognition of the microstructures by the growth cones and neurites.

Animals↗
PubMed1988

[Morphological characteristics of the left ventricle with volume-and pressure-overload in valvular heart diseases].

There are some cases in which pump function of the left ventricle (LV) is not sufficiently improved despite of successful operation, especially in advanced hypertrophy. The impaired pump function is believed to result from irreversible myocardial damage. Thus, the present study is designed to make clear the histopathological changes of the LV muscle in hypertrophied hearts. Fifty-six patients with volume-overloaded hearts (27 with AR, 24 with MR, and 4 with AR and MR) and 10 patients with AS were examined. Cardiac muscle specimens for histological examination were obtained from the LV anterior wall. In volume-overloaded hearts, the muscle cell diameters were correlated with both LV dimension (LVDd and LVDs) and LV volume index (LVEDVI and LVESVI). The progress of interstitial fibrosis was correlated with LV volume index. Five of the 9 patients with massive myocardial hypertrophy (in all cases, muscle cell diameter was greater than 30 microns) did not show any improvement in histological figure after operation and displayed persistent postoperative LV dilatation. Patients with either LVDd greater than 75mm and LVDs greater than 55mm or LVEDVI greater than 200ml/m2 and LVESVI greater than 100ml/m2, whose hearts showed massive myocardial hypertrophy and severe fibrosis, tended to have postoperative cardiac dysfunction. In pressure-overloaded hearts, the muscle cell diameters were correlated with LVPWTh and LVESVI. Noteworthily, fibrosis and degeneration of muscle fibers were slight as compared with advanced myocardial hypertrophy.

Cardiomyopathy, Hypertrophic↗
PubMed1987

Transformation of glial cells in mouse embryonic brain cells in vitro with simian virus 40.

Embryonic (E10) mouse cerebellum and spinal cord cells were cultured and infected with simian virus 40 (SV40). In all infected dishes, rapid proliferations (a dividing time less than 18 h) of non-neuronal cells were detected. Three populations of those cells, SVa, SVb and SVc, could be passaged more than 10 times. They had flat morphologies like young astrocytes and, indeed, showed immunoreactivities to glial fibrillary acidic protein (GFAP), a marker for the astrocyte. However, they had an ability to form colonies in a soft agarose medium. The large-T antigen could be detected in nuclei of the three cell populations by a fluorescent antibody test and immunoprecipitation. No transformation of neuronal cells was detected, nevertheless there were neuronal cells still possessing dividing capacities in the culture preparation.

Animals↗
PubMed1987

Presynaptic Ca-antagonist omega-conotoxin irreversibly blocks N-type Ca-channels in chick sensory neurons.

The present studies on electrophysiological and pharmacological differences of the three types of Ca-currents (N-, L- and T-types) in whole-cell clamped, cultured embryonic chick sensory neurons revealed that the majority (94%) of the Ca-currents in the nerve cells were the N-type, omega-Conotoxin (omega CTX, 5 microM), a blocker of transmitter release at the presynaptic terminals, induced a complete and irreversible blockage of Ca-currents elicited from the resting membrane potential (-60 mV) in 29 cells among 58. The Ca-currents thus irreversibly blocked by the omega CTX were determined as the N-type (neuronal), as they were insensitive to nifedipine (5 microM) or were reduced in amplitude by Bay K 8644 (5 microM). A small fraction (12%) of the total Ca-currents, which were still present after the omega CTX treatment (in the rest of 29 cells), were pure L-type (long-lasting) Ca-currents, as they were enhanced by the Bay K and were blocked by the nifedipine. omega CTX was a partial and reversible blocker of the L-type Ca-currents. Furthermore, T-type (transient) Ca-currents elicited in the hyperpolarized membrane (at -100 mV) were blocked by omega CTX in an incomplete and reversible manner. The N-type Ca-currents thus separated in the nerve cells exhibited various differences in features of the voltage-dependence and ionic selectivity from the L- and T-type Ca-currents.

Animals↗