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Biomedical subjects

J Fujimoto

Publications and source records attributed to J Fujimoto.

At least 361 records · Page 20Linked to original sources
PubMed1981

Immunochemical characterization of human TL-like (T48) and Ly 1-like (T72) glycoproteins using two-dimensional polyacrylamide gel electrophoresis.

Xenoantisera, designated AT48 and AT72, were developed by immunizing rabbits with human thymus cell membrane and guinea-pigs with a T-cell glycoprotein purified from leukaemic T-cell membrane. Whereas AT48, after appropriate absorption, reacted exclusively with the majority of thymocytes (mainly cortical thymocytes) among normal lymphoid populations, AT72 reacted with virtually all of the thymus and T cells but not with B cells. Thymocytes, which were strongly reactive with AT72, existed in the thymic medulla, but cortical cells were also very weakly reactive with AT72. When cultured T-cell lines, all of which were derived from patients with T-cell-type acute lymphatic leukaemias, were tested for their reactivities with AT48 and AT72 by immunofluorescence, we found that AT48 stained certain T-cell lines, whereas AT72 stained all of the T-cell lines tested so far. Immunochemical data showed that AT48 precipitated a 48K molecular weight (mol. wt) glycoprotein from 125I-labelled thymus cell surface glycoproteins, which appeared to be very weakly associated with a 12K mol. wt component. These 48K and 12K mol. wt components precipitated by AT48 showed almost identical isoelectric points (pI) to those of HLA heavy chain and beta 2-microglobulin respectively. AT72, on the other hand, precipitated a 72K mol. wt glycoprotein from thymus and T cells as well as from leukaemic T cells. A less prominent 65K mol. wt glycoprotein was also precipitated by AT72 from thymus and T cells but not from leukaemic T cells. These two components showed almost identical pI ranging approximately from 4 to 7, and this marked charge heterogeneity observed was reduced by neuraminidase treatment, suggesting that it reflects the heterogeneity in sialylation of this molecular species. We concluded from these data that AT48 and AT72 used in this work could detect human homologues of mouse TL and Ly 1 antigens respectively.

Antigens, Differentiation, T-Lymphocyte↗
PubMed1981

Isolation and partial characterization of a 72,000-dalton glycoprotein (Tgp72) on human thymus and T cells: possible relationship to mouse Ly-1 antigens.

A xenoantiserum was raised in guinea pigs to a human T cell antigen (Tgp72) partially purified from cell membrane of T cell leukemia cells from a patient with chronic lymphosarcoma cell leukemia of T cell-type by using sequential chromatographic procedures. The antiserum reacted with all of human thymus and peripheral T cells as well as with a variety of cultured leukemic T cell lines, but not with B cells, granulocytes, and other cultured lymphoid cell lines, including normal B cell, Burkitt, myeloid, and non-T non-B cell lines. Immunoprecipitated material from 125I-labeled thymocyte, T cell, and T cell leukemia cell-surface glycoproteins by this antiserum was analyzed by SDS-polyacrylamide gel electrophoresis, which disclosed a single component with a m.w. of 72K (Tgp72). Further immunochemical data suggest that Tgp72 antigen on human thymus and T cells is an acidic glycoprotein composed of a single polypeptide rather than sulfhydryl-linked subunits, showing a marked electric charge heterogeneity in DEAE anion-exchange resin chromatography. This is further confirmed by the data obtained from 2-dimensional polyacrylamide gel electrophoresis, indicating that Tgp72 is a 72K m.w. glycoprotein with an isoelectric point ranging from 4 to 7. Because of its similarity in tissue distribution and immunochemical characteristics, Tgp72 on human thymus and T cells was assumed to be a human homologue of Ly-1 antigens in murine system.

Animals↗
PubMed1980

Epidemic of hand, foot and mouth disease in Gifu Prefecture in 1978.

During the period from May to August, 1978, an epidemic of hand, foot and mouth disease (HFMD) occurred in Gifu prefecture. Epidemiological, virological and serological investigations were performed. Cases involved ranged from 0 to 31 years of age, and 80.2% of them were under 5 years of age. The incidence of HFMD with neurological complication (3.7%) was lower than that in 1973. Enterovirus 71 (EV71) was isolated from 83 of 108 cases (75.9%) and a significant rise in the neutralization antibody titer against the isolate was found in 14 of 25 cases (56%). Thus, it was confirmed that the causative agent of the epidemic of HFMD in Gifu prefecture in 1978 was enterovirus 71.

Adolescent↗
PubMed1978

A comparison of pin-retained amalgam and composite resin cores.

The 24 hour tensile loads required to separate the core material and dentin produced the following results: 1. No statistical difference was found between the tensile strength of retained amalgam and composite resin core materials. 2. Generally, no statistical difference was found between the retentive ability of three and four pins. 3. No statistical difference was found between the retentive ability of regular pins and minim pins if three or more pins were employed. 4. As the number of regular pins was increased beyond three, and when four minim pins were used, there was a very significant increase in dentin fracture.

Composite Resins↗
PubMed1976

Intracellular distribution of (14C)bleomycin and the cytokinetic effects of bleomycin in the mouse tumor.

The differential effects (BLM) on cycling and noncycling cells were investigated with a mouse ascites tumor in vivo. An i.p. injection of 37.0 or 111.1 mug BLM per g caused a decrease in tumor cell number but an increase in percentage of tumor cells in mitosis. There are no significant differences between the percentage labeled mitoses at various times after pulse labeling by tritiated thymidine of BLM-treated tumor cells and by that of an untreated control, except that the height of the second peak was significantly lower in the treated cells. Hence BLM may be cell cycle nonspecific, and the BLM-induced decrease in cell number, i.p., may stimulate some nondividing cells to reenter the division cycle. However, the fact that percentage of cells in mitosis versus time after the administration of BLM showed two peaks indicates the possibility that another cause of the increase in mitotic figures might be a relative increase of cycling cells due to higher sensitivity of noncycling cells to the agent. Autoradiographic studies on the intracellular distribution of [14C]BLM revealed the following. (a) There were few necrotic cells in mitosis that incorporated much [14C]BLM into the cytoplasm at each time point and the mitotic figures gradually increased with time after i.p. injection of the isotope, while necrotic cells other than in mitosis, most of which were heavily labeled, increased in number with time. These findings seem to be related to the possibility that cycling cells may be less sensitive to BLM. The mode of intracellular distribution of [14C]BLM in mitotic cells changed with time and appeared to reflect the drug susceptibility depending on the cell cycle phase when labeled.

Animals↗