Search PubMed⌕ Search

Biomedical subjects

J Frucht-Pery

Publications and source records attributed to J Frucht-Pery.

At least 55 records · Page 3Linked to original sources
PubMed1994

The use of low-dose mitomycin C for prevention of recurrent pterygium.

BACKGROUND: The administration of high doses of topical mitomycin C after pterygium excision causes a variety of complications. METHODS: Seventy-five patients who had advanced or recurrent pterygia underwent excision of pterygia, leaving the sclera bare. Patients were randomized in a masked fashion into three groups of 25 patients each. Patients in group 1 received topical 0.01% mitomycin C for 5 days, patients in group 2 received 0.02% mitomycin C for 5 days, and those in group 3 were treated with 1200 rad beta-irradiation. Patients were followed by a surgeon who was masked to the patient treatment. The mean follow-up period was 15.3 months. RESULTS: The recurrence rate after pterygium surgeries was 8% in group 1, 4% in group 2, and 20% in group 3. There were no statistical differences between the study groups. Delay of epithelialization for 8 weeks in one patient and degenerative calcification of conjunctiva 18 months after surgery in another patient, both from group 2, were the only complications in this study. CONCLUSION: This study indicates the advantage of 0.01% mitomycin C for post-operative prevention of recurrence of pterygium.

Administration, Topical↗
PubMed1994

Single dosage of mitomycin C for prevention of recurrent pterygium: preliminary report.

We studied the recurrence rate of pterygium after administration of a single dosage of topical mitomycin C at the completion of pterygium surgery. Forty patients who had advanced or recurrent pterygium underwent excision of pterygium, leaving the sclera bare. Patients were randomized in a masked fashion into two groups of 20 patients each. Group 1 received a single dosage of topical 0.02% mitomycin C for 5 min at the completion of the surgery whereas group 2 underwent the same procedure but received NaCl 0.9% instead of mitomycin C. Patients were followed from 6 to 15 months in a masked manner. The pterygium recurred in one patients (5%) in group 1, whereas in group 2 the recurrence rate was 45% (p = 0.0035). Formation of dellen and a delay of epithelialization for 5 weeks were found in one patient in group 2. No complications were observed in patients in group 1. This study indicates the possible advantage of administration of a single dosage of 0.02% mitomycin C for postoperative prevention of recurrence of pterygium.

Adult↗
PubMed1994

Physicochemical characterization and acute toxicity evaluation of a positively-charged submicron emulsion vehicle.

Fine, homogeneous, positively-charged emulsions with a mean droplet size of 138 +/- 71 nm and a zeta potential value of 41.06 mV were prepared using a combination of emulsifiers comprising phospholipids, poloxamer 188, and stearylamine. The pH of these emulsions decreased with time. However, the extent of decrease was dependent on the storage temperature. The mean droplet size of the emulsions that had been prepared with 1% poloxamer began to increase slightly after six months' storage, particularly when stored at 23 and 37 degrees C. However, emulsions prepared with 2% poloxamer remained stable for at least 10 months at 4 degrees C, suggesting that the poloxamer 188 concentration is critical for prolonged emulsion stability. The results of the ocular tolerance study in rabbit eye indicate that hourly administration of a positively-charged emulsion vehicle was well tolerated without any toxic or inflammatory response to the ocular surface during the five days of the study. Scanning electron microscopy revealed a normal corneal surface, which was not different from that of the animals treated with physiological saline. No marked acute toxicity was observed when 0.6 mL of positively-charged emulsion was injected intravenously to BALB/c mice. Furthermore, no difference was noted between this group of animals and the group injected with the marketed Intralipid emulsion. These results were further confirmed in a rat study where there were no deaths following intravenous injection of 3.3 mL per rat of the positively-charged emulsion or Intralipid. Neither emulsion elicited any hepatotoxic or nephrotoxic effects. The overall results suggest that the novel positively-charged emulsion is suitable for parenteral use, and for ocular application.

Amines↗
PubMed1994

Entactin/nidogen: synthesis by bovine corneal endothelial cells and distribution in the human cornea.

PURPOSE: The purposes of this study were to determine whether entactin/nidogen (E/N) is synthesized and secreted by corneal endothelial cells and to characterize the distribution of E/N in the human cornea. METHODS: Cultured bovine corneal endothelial cells were metabolically labeled with [35S]methionine. Newly synthesized E/N was detected in cell lysates and culture medium by immunoprecipitation, using monoclonal anti-E/N antibodies, polyacrylamide gel electrophoresis, and autoradiography. The presence of E/N in the subendothelial extracellular matrix was demonstrated by Western blot analysis of solubilized extracellular matrix proteins. The distribution of E/N in normal human corneas was studied by indirect immunofluorescent staining of frozen sections, using monospecific anti-E/N antibodies. RESULTS: E/N was detected in the basement membrane (BM)-like extracellular matrix deposited by corneal endothelial cells, as well as in cell lysates and culture medium. Immunofluorescence studies revealed the presence of E/N in both the epithelial and endothelial BM and to a much lower extent in the stroma. E/N was detected throughout the thickness of the epithelial BM, but its staining decreased in intensity toward the central part of the cornea. In the endothelial BM (Descemet's membrane), E/N fluorescence was limited to its most posterior portion, produced postnatally. CONCLUSIONS: Corneal endothelial cells synthesize and secrete E/N, E/N was found in both the epithelial and endothelial basement membranes but was primarily localized to the posterior portion of Descemet's membrane and the periphery of the epithelial BM. The authors suggest that E/N may be important in healing processes of corneal injuries and in the pathogenesis of diseases involving the postnatal region of Descemet's membrane.

Animals↗
PubMed1993

Efficacy of doxycycline and tetracycline in ocular rosacea.

We compared the effects of doxycycline and tetracycline hydrochloride on the subjective symptoms in ocular rosacea. Twenty-four patients with symptomatic ocular rosacea were randomly assigned to two groups and treated with doxycycline 100 mg/day (group 1, 16 patients) or tetracycline hydrochloride 1 g/day (group 2, eight patients). The dosages of each drug were gradually tapered and discontinued according to symptomatic response. At each examination all the manifesting symptoms were scored by the patients. Patients were followed up from six weeks to three years. After six weeks of drug treatment, all patients except one had symptomatic improvement. Although most of the scores of the symptoms were significantly decreased in both groups, greater symptomatic relief occurred in the tetracycline hydrochloride-treated patients (P = .041). However, after three months of treatment there was no significant difference in symptoms between the two groups. Gastrointestinal tract complications occurred in two of the 16 patients (12.5%) in group 1 and in three of the eight patients (37.5%) in group 2. Both tetracycline hydrochloride and doxycycline can control the symptoms of ocular rosacea.

Administration, Oral↗
PubMed1993

Effect of eye patching on ocular surface.

We studied the effect of pressure patching in 27 medical students. Fourteen students had pressure patching in one eye (group 1) and 13 (group 2) had light patching. The fellow eye of each volunteer remained unpatched (group 3). Clinical signs and symptoms were scored and evaluated at the first and the following (after overnight) examinations. Before patching no subject had any of the clinical signs or symptoms. After the patch was removed in groups 1 and 2, all except one volunteer in each group had clinical signs or symptoms, or both, whereas in group 3, none had clinical signs or symptoms. Group 1 had greater scores for clinical signs (P = .019) and for symptoms (P = .038) as compared to group 2. In group 1, two participants did not complete the study period (by removal of the patch) because of severe discomfort and three had temporary irregularities in corneal surface with temporary decrease of vision. We suggest that pressure patching may cause discomfort and changes in visual acuity that are usually attributed to other reasons.

Adolescent↗
PubMed1993

The effect of the ArF excimer laser on Candida albicans in vitro.

Candida albicans colonies in Sabouraud agar plates were irradiated with the excimer laser as follows: (a) at 10 Hz, power densities of 115-300 mJ/cm2 and 200-3000 pulses, and (b) power density of 115 mJ/cm2 and 200 pulses at 10-50 Hz. Additional colonies were irradiated with a power density of 200 mJ/cm2 at 10 Hz and 500 pulses. Each colony was cultured and the visible colony forming units were counted after 24 h. The cultures remained sterile at: 115 mJ/cm2, 1500 or 3000 pulses; 200 mJ/cm2, 400 or 500 pulses; and 300 mJ/cm2, 300 or 400 pulses. They decreased significantly in other groups. Photoablation with a power density of 115 mJ/cm2 and 200 pulses significantly decreased the number of yeast colonies in the culture plates at 30 Hz (p < 0.029) and 50 Hz (p < 0.02). Photoablation did not affect the counts in colonies located 1 or 2 mm from the treated colonies. Various energies of the excimer laser may significantly reduce or eliminate yeast in vitro.

Candida albicans↗
PubMed1993

Wedge resection for postkeratoplasty astigmatism.

Twelve patients underwent a modified wedge resection after keratoplasty; 11 of them were followed for 6 to 24 months. The average preoperative astigmatism was 15.40 diopters (range, 12.50 to 22.00 D). The wedge was resected from the donor side and the wound closed with 10-0 or 11-0 Mersilene sutures. Postoperatively, the average astigmatism was 4.36 D (range, 1.50 to 9.00 D), and the visual acuity, corrected with spectacles, was 20/40 or better in five cases (45%). Complications included high residual astigmatism or irregular astigmatism and extensive scarring due to the use of Mersilene sutures. Overall, modified resection of the wedge is an effective technique for managing postkeratoplasty high astigmatism.

Adult↗
PubMed1993

Cataract surgery in a leprosy population in Liberia.

In Liberia, 43 eyes of 30 patients with ocular leprosy underwent cataract extraction; 33 eyes had extracapsular cataract extraction (ECCE) and 10 eyes had intracapsular cataract extraction (ICCE). ICCE was performed in eyes with poor visualization of the anterior chamber. In 95% of the eyes, the postoperative vision improved by 2 Snellen lines or more, but functional visual acuity (better than 20/200) was achieved in only 65% (82% post-ECCE and 10% post-ICCE). Fewer postoperative complications were observed after ECCE. These findings may have been related to less ocular involvement by leprosy preoperatively. ECCE should be attempted when the visualization of the anterior chamber is fair.

Adult↗
PubMed1992

Running nylon suture dissolution after penetrating keratoplasty.

We attempted to evaluate whether the low degrees of astigmatism achieved early in the postkeratoplasty period with the combined interrupted/running suturing technique were maintained for long periods of time. For 13 to 70 months (mean, 30.2 months), we monitored a group of patients (25 eyes) who had previously undergone the combined interrupted/running suturing technique (12 interrupted 10-0 nylon sutures and one running 11-0 nylon suture). Nine running sutures broke spontaneously, causing a significant increase of the keratometric astigmatism of the entire population from 1.7 +/- 1.6 to 3.4 +/- 2.6 diopters (mean +/- standard deviation). The mean vector-corrected change in astigmatism after suture breakage was 4.9 +/- 2.6 diopters. Surgical procedures to reduce astigmatism were required in many of the eyes in which the 11-0 running nylon suture broke. Our results suggested that 11-0 nylon is not an ideal material for the running suture because its high rate of spontaneous disruption leads to undesired, statistically significant increases in postkeratoplasty astigmatism. Further, our results indicated that the selective suture technique can maintain low degrees of astigmatism only if the sutures remain intact. Studies of the effect of keratoplasty suturing techniques on astigmatism should probably include a follow-up that is sufficiently long to indicate its long-term value to the patient.

Adult↗
PubMed1992

The effect of indomethacin on prostaglandin E2 in human cornea and conjunctiva.

We studied the inhibitory effect of topical 1% indomethacin aqueous suspension on prostaglandin E2 in 20 conjunctivae extracted during cataract surgery and 16 corneas extracted during penetrating keratoplasty. Thirty minutes before each surgical procedure, indomethacin or placebo (0.9% sodium chloride solution) was instilled into the eye to be operated on in a masked fashion for conjunctiva and selectively for cornea. The excised corneal buttons and conjunctival tissue were homogenized and assayed for prostaglandin E2 concentration using radioimmunoassay. The levels of prostaglandin E2 in the conjunctiva significantly decreased (P less than .0033) after administration of indomethacin (203.7 +/- 219.5 pg/mg) compared with the placebo-treated tissues (849.8 +/- 564.1 pg/mg). The levels of prostaglandin E2 in the corneas significantly decreased (P less than .0157) after administration of indomethacin (522.5 +/- 570.2 pg in the cornea) compared with the placebo-treated tissues (1636.3 +/- 1344.1 pg in the cornea). Indomethacin may serve as a potent anti-inflammatory agent in conjunctival and corneal inflammatory disorders.

Adolescent↗
PubMed1991

The effect of topical administration of indomethacin on symptoms in corneal scars and edema.

We conducted a masked randomized study of 50 patients to evaluate the effect of administration of topical indomethacin 1% suspension on symptoms in corneal scars, edema, infiltrates, and erosions. Patients with symptoms (photophobia, pain, itching, burning sensation, foreign-body sensation, and tearing) were treated with topically administered indomethacin 1% or placebo and monitored for eight weeks. The severity of the complaints was rated and the scores were evaluated (Wilcoxon rank-sum test). Of the 25 patients treated with indomethacin, 21 (84%) had improvement in symptoms and the severity of each of the symptoms was significantly decreased. Of the 25 patients treated with placebo, one (4%) had improvement in symptoms without statistical change of the severity of the symptoms. However, when the placebo-treated patients received indomethacin drops, the symptoms were significantly decreased (P less than .002). This study suggests that topical administration of indomethacin 1% may reduce ocular symptoms in patients with corneal scars, edema, or erosions.

Administration, Topical↗
PubMed1990

Experimental radial thermokeratoplasty in rabbits.

Radial thermokeratoplasty is a new technique designed to reduce hyperopia. We evaluated the histopathological effect at 2, 4, and 30 days following radial thermokeratoplasty in rabbit corneas. Surgical complications included variable-sized coagulations and microperforations in two eyes. Light microscopy disclosed that the burns were 90% in depth and healing occurred by epithelial ingrowth. The stroma surrounding the coagulation was acellular early but keratocyte repopulation was apparent by 30 days. A retrocorneal membrane four to five cells thick was noted at the early time points; however, by 30 days only a double layer of spindle-shaped cells remained. By electron microscopy, damage to surrounding endothelial cells was estimated to be 300 microns (from the center of the coagulation). Our results suggest that radial thermokeratoplasty, which utilizes a temperature of 600 degrees C placed within the cornea to a depth of 90%, will cause significant damage to the corneal endothelium beneath and surrounding the coagulation site.

Animals↗
PubMed1989

The effect of increased intraocular pressure on visual acuity and corneal curvature after radial keratotomy.

To detect the effect of increased intraocular pressure on visual acuity and corneal curvature after radial keratotomy, we measured these variables in the sitting and inverted positions in 18 patients who underwent radial keratotomy (Group 1) and compared their results with those from the unoperated on eyes of seven patients (Group 2). We also compared the results before and after inversion within each group. Intraocular pressure increased to approximately two times normal in each group. Significant improvement in visual acuity and reduction in central keratometry were noted only in Group 1. By multiple regression analysis, visual improvement correlated with the number of incisions but not the time since surgery. Our study provides evidence that increased intraocular pressure may account for transient changes in vision and corneal curvature after radial keratotomy.

Adult↗
PubMed1989

The effect of pterygia on contrast sensitivity and glare disability.

To detect subtle changes in visual acuity, we measured spatial contrast sensitivity in 12 patients with pterygium (Group 1) and compared their results with those from an age- and sex-matched control population (Group 2). Glare disability was also measured in each group. Measurements of contrast sensitivity were lower in Group 1 at all spatial frequencies than those of Group 2, independent of the testing device used. Glare disability was significantly increased in Group 1 compared to Group 2. Our studies indicate that contrast sensitivity and glare disability testing may provide additional objective methods for documenting impaired vision in patients with pterygium when Snellen visual acuity is minimally affected.

Adult↗