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Biomedical subjects

J Frick

Publications and source records attributed to J Frick.

215 records · Page 12Linked to original sources
PubMedDate not supplied

Effect of gossypol on testicular testosterone production in vitro.

Testicular minces were utilized to study the effect of gossypol on testosterone production. Testosterone production was assessed in both control nd gossypol treated groups after 0 to 4 hours incubation in the presence of hCG. Media testosterone was measured by radioimmunoassay. Gossypol did not alter testosterone production when present in incubates at the concentrations of 3.5 x 10(-5) M, 7 x 10(-5) M and 3.5 x 10(-4) M. Preincubation of testis mince with gossypol (7 x 10(-6) M, 7 x 10(-5) M, 3.5 x 10(-4) M) for 1 to 4 hours did not alter subsequent hCG induced testosterone production in mature rats. Testosterone production however, was inhibited in immature rat testis when the whole testis was incubated for 4 hours with different concentrations of gossypol (7 x 10(-6) M, 7 x 10(-5) M, 3.5 x 10(-4) M). In vivo testosterone production was not inhibited in the immature rat testis 24 hours after oral administration of gossypol (100 mg/kg).

Animals↗
PubMedDate not supplied

Occurrence of Clostridium difficile toxin-associated gastroenteritis following antibiotic therapy for otitis media in young children.

The pathogenesis of diarrhea following antibiotic therapy for otitis media in young children remains unknown. We performed a prospective study evaluating the incidence of diarrhea and Clostridium difficile toxin in 115 outpatients (ages 6 months to 6 years) with acute otitis media treated with ampicillin, amoxicillin or trimethoprim-sulfamethoxazole. In 21 patients younger than one year of age six of 11 developing diarrhea had toxin-positive stools compared with three of 10 without diarrhea (P = 0.39). In 94 patients between 13 months and 6 years of age three of 12 with diarrhea had toxin-positive stools compared with five of 82 without diarrhea (P = 0.06). Diarrhea was self-limited in all cases. Although the data suggest that C. difficile might have been associated with diarrhea in the older children, further studies will be required to confirm this finding.

Amoxicillin↗
PubMedDate not supplied

Antifertility efficacy of gossypol acetic acid in male rats.

Fertility response and sperm motility of male Wistar rats exposed to various concentrations (2.5 - 30 mg / kg / day) of gossypol acetic acid (GAA) was studied. GAA was given orally. A marked reduction in fertility occurred when rats received more than 15 mg / kg / day of GAA for 5 - 10 weeks (p less than 0.01); dosage below 15 mg had no antifertility effect even after 20 weeks. There is some evidence that GAA administration (30 mg / kg) every other day for 10 weeks had no antifertility effect. A clear relationship between the inhibition of sperm motility and GAA administration was observed (p less than 0.01). The interrelationships between fertility, sperm motility and GAA treatment were analyzed by Yates' chi-square test and Fisher's exact test.

Animals↗
PubMedDate not supplied

Toxicity of gossypol at antifertility dosages in male rats: statistical analysis of lethal rates and body weight responses.

The lethal rates and body weight responses of male Wistar rats following oral treatment with gossypol acetic acid (GAA) at dosages of 2.5-30 mg/kg were analysed by various statistical procedures. Daily treatment with GAA for 10 weeks was associated with lethal rates significantly different from placebo groups in both the low dose (2.5, 5.0, and 7.5 mg/kg, p less than 0.05) and high dose groups (15, 20 and 30 mg/kg, p less than 0.01). The pronounced mean body weight gain observed in placebo groups, was suppressed in the GAA-treated groups at each dose level after 10 weeks. This indicates decreased appetite and/or lessened feed utilisation caused by GAA activity. GAA administration at a dose of 30 mg/kg every other day exerted identical deleterious effects as daily treatment. As derived from the antifertility responses of the GAA treatment groups it was evident that the toxic activity of GAA was not related to its antifertility efficacy. It is concluded that GAA has remarkable toxicity at antifertility dosages in rats.

Administration, Oral↗
PubMedDate not supplied

Effect of human gamma interferon on mice testis: a quantitative analysis of the spermatogenic cells.

Effect of Human Gamma Interferon (Hu-IFN-gamma) on the testicular histology was studied in mice. Male mice were administered Hu-IFN-gamma intratesticularly at the doses of 2, 10 and 20 micrograms/testis in a volume of 1.0 microliter isotonic normal saline. Contralateral testis served as control and was administered same amount of vehicle. All the animals were sacrificed 7 days after drug administration. Body weight and the weights of testis and epididymis were not affected by IFN treatment nor was there any effect of the drug on the motility of the vas deferens spermatozoa. Low dose of IFN (2 micrograms) did not have significant effect on the histoarchitecture of the testis and various spermatogenic elements, a progressive damage was however observed with the increasing doses of IFN. Pronounced deleterious effect of IFN on the testis leading to desquamation of the germinal epithelium, reduction in the germinal cell height and tubular diameter was observed with 20 micrograms dose. Quantitative studies on seminiferous epithelium showed a significant decrease in the number of Sertoli cells, stage-7 spermatids and stage-16 spermatozoa. The ratios of resting type spermatocyte: type A spermatogonia and stage-7 spermatids: pachytene spermatocyte was also reduced. The ratios of pachytene spermatocyte: resting spermatocyte and stage-16 spermatozoa: stage-7 spermatids were however not affected by IFN treatment. In another experiment IFN was administered (2 micrograms/day) subcutaneously to male mice for 30 days. No effect of drug treatment on body weight, organ weight, sperm motility and histology (including morphometry) of the testis was observed. Our data suggest that IFN action at testis may be associated with the antiproliferative effect of interferon.

Animals↗
PubMedDate not supplied

Bioassay for determination of human serum luteinizing hormone (LH): a routine clinical method.

Serum levels of LH are used as marker of a number of pathological conditions. In the past many methods (RIA, IRMA, ELISA) have been employed to measure serum LH, these procedures, however, suffer from the drawback that they determine the immuno reactive and not the bioactive part of the hormone. An improved in vitro bioassay method for the estimation of serum LH has been described. The underlying mechanism of the assay is testosterone production by mouse Leydig cells in the presence of added LH. The method has been significantly improved in terms of sensitivity (0.2 IU/l) and simplicity; the assay is simple and does not require any special instruments and can be set up in any endocrinological laboratory.

Animals↗
PubMedDate not supplied

Effect of gossypol on immature male rats.

In immature male rats the body growth rate, the testis and epididymis weight were not affected by gossypol treatment (4, 8 and 40 mg/kg body weight/24 hrs. for 30 days). There was however a marked reduction in the weight of the prostate after high dose gossypol administration. Furthermore gossypol treatment did not show any effect on the histoarchitecture of the testis nor did the drug treatment have any effect on sperm motility. The plasma levels of testosterone, LH and FSH in gossypol treated animals were no different from those on the controls.

Animals↗
PubMedDate not supplied

[Hormone determinations in andrology].

The measurements of the pituitary and peripheral plasma hormone values represent an important part in the andrological armamentarium. The basal plasma hormone levels in different urological and andrological disorders are demonstrated as well as the interpretation of the LH-RH test in Sertoli-cell-only- and Klinefelter's syndrome. Our days endocrinological diagnostic procedures are based on the determination of important hormonal parameters.

Adult↗
PubMedDate not supplied

Neuropeptides in the human penis: an immunohistochemical study.

In the present study, the distribution of neuropeptides in the human penis is demonstrated by immunohistochemistry (IHC). IHC screening detected a complex network of nerve fibers containing vasoactive intestinal polypeptide (VIP), peptide histidine-methionine (PHM), prepro-VIP (111-122), neuropeptide Y (NPY), C-flanking peptide of NPY (C-PON), calcitonin gene-related peptide, substance P, and galanin immunoreactivities. Special attention was also given to the recently isolated, VIP-related lizard peptide helospectin, which could also be detected in neuronal elements in the penis. Colocalization studies showed the coexistence of VIP, PHM, and partly helospectin, and of NPY with C-PON within nerve fibers in the cavernous and spongious body, the glans penis, and the urethra.

Adult↗
PubMedDate not supplied

Effect on human gamma interferon on mice testis: a quantitative analysis of the spermatogenic cells.

Effect of Human Gamma Interferon (Hu-IFN-gamma) on the testicular histology was studied in mice. Male mice were administered Hu-IFN-gamma intratesticularly at the doses of 2, 10 and 20 micrograms/testis in a volume of 1.0 microliter isotonic normal saline. Contralateral testis served as control and was administered same amount of vehicle. All the animals were sacrificed 7 days after drug administration. Body weight and the weights of testis and epididymis were not affected by IFN treatment nor was there any effect of the drug on the motility of the vas deferens spermatozoa. Low dose of IFN (3 mukg) did not have significant effect on the histoarchitecture of the testes and various spermatogenic elements, a progressive damage was however observed with the increasing doses of IFN. Pronounced deleterious effect of IFN on the testis leading to desquamation of the germinal epithelium, reduction in the germinal cell height and tubular diameter was observed with 20 micrograms dose. Quantitative studies on seminiferous epithelium showed a significant decrease in the number of Sertoli cells, stage-7 spermatids and stage-16 spermatozoa. The ratios of resting type spermatocyte: type A spermatogonia and stage-7 spermatids: pachytene spermatocyte was also reduced. The ratios of pachytene spermatocyte: resting spermatocyte and stage-16 spermatozoa: stage-7 spermatids were however not affected by IFN treatment. In another experiment IFN was administered (2 micrograms/day) subcutaneously to male mice for 30 days. No effect of drug treatment on body weight, organ weight, sperm motility and histology (including morphometry) of the testis was observed. Our data suggest that IFN action at testis may be associated with the antiproliferative effect of interferon.

Animals↗