[Results of enzootic bovine leukosis control in contaminated herds using serologic methods].
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Biomedical subjects
Publications and source records attributed to J Franz.
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The radioimmunologic assay (RIA) was elaborated for a demonstration of serum antibodies to bovine leukosis virus. The procedure makes use of the viral antigen bond to the fixed phase of a polystyrene carrier. The method was compared with the ELISA method and pseudoneutralizing and immunodiffusion tests. High congruence of the results of the RIA and ELISA methods was achieved, making 95%. The RIA method is more sensitive than the immunodiffusion test.
There is a description of the enzymoimmunologic method (ELISA), which was used for demonstration of rotaviruses and coronaviruses in the samples of excrements of the calves suffering from diarrheas. It is shown by a comparison with the results obtained by electron microscopy that the ELISA method provides by up to 50% higher capture rate and the reaction is highly specific. The method can also be applied to a detection of human rotavirus in the children's stool.
Photoacoustic-spectroscopic investigations of the penetration of topically applied drugs into the horny layer of the human skin are reported. By this technique the light absorption of the horny layer and its change due to drug diffusion can be determined in vivo in dependence on depth. Measurements have been performed on a sunscreening agent and on an UVA-absorbing systemic drug after topical application to the forearm.
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