Ecto-5'nucleotidase and adenosine deaminase activities of lymphoid cells.
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5'-Nucleotidase↗
Biomedical subjects
J Favero
Publications and source records attributed to J Favero.
Unmasking of membrane enzyme activities and the problem of subcellular localization of adenylate cyclase in pig lymph node lymphocytes.
A large-scale purification of plasma membranes from pig lymph node lymphocytes is described. Centrifugation on a discontinuous sucrose density gradient was performed in a zonal rotor. Adenylate cyclase activity of untreated fractions displayed a profile different from that of plasma membrane enzymatic markers and was maximal at higher density. However, when latent adenylate cyclase was unmasked by Lubrol PX treatment, its maximum was shifted to lower density and was no longer significantly different from that of plasma membrane markers. These results are discussed in terms of cell surface topography.
3',5'-Cyclic-AMP Phosphodiesterases↗
Microbiological transformation of 1,2-diphenyl-3,5 dioxo-4-n-butyl pyrazolidine (phenylbutazone).
Microbiological conversion of phenylbutazone with Rhizopus arrhizus Fischer gave 4-hydroxy phenylbutazone in excellent yield. Spectral studies, mass spectrometry, in particular, were made of the obtained metabolite; other experiments performed with the intact microorganism or with the cell-free system, as well as studies with the well-known metabolic inhibitor, SKF 525 A, indicated that the obtained 4-hydroxy phenylbutazone was not an artefact. Similar experiments were performed on oxyphenbutazone.
Biotransformation↗
Lymphocyte membrane adenosine receptors coupled to adenylate cyclase: properties and occurrence in various lymphocyte subclasses.
Lymphocytes possess membrane adenosine receptors through which this nucleoside or analogs activate adenylate cyclase. Several adenosine analogs - 5'-N-ethylcarboxamide adenosine (NECA), N6-(L-2-phenyl-isopropyl) adenosine (PIA), 2-chloro adenosine (Cl-Ado) - were tested on the adenylate cyclase of mouse thymocyte homogenates; the order of potencies: NECA greater than Cl-Ado greater than PIA is consistent with the order which has been assumed to be characteristic of Ra type adenosine receptors. The stimulation of thymocyte adenylate cyclase by NECA was biphasic while the responses to Cl-Ado and PIA were not, which suggests the existence of Ra sub-types of adenosine receptors. Moreover, these adenosine receptors were found to be present in several lymphocyte subclasses: mature and immature mouse thymocytes, B and T mouse splenocytes, human peripheral blood lymphocytes, and also in lymphoblastoid cell lines. No crucial differences were observed between the adenylate cyclase stimulations of the various population by NECA.
Adenosine↗