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Biomedical subjects

J Földes

Publications and source records attributed to J Földes.

At least 19 recordsLinked to original sources
PubMed1992

Biologic markers in blood reflecting thyroid hormone effect at peripheral tissue level in patients receiving levothyroxine replacement for hypothyroidism.

Plasma fibronectin, serum procollagen-III-peptide and sex-hormone binding globulin as not specific markers of thyroid hormone effect at peripheral tissue level were determined and their values were related with serum levels of TSH, free-thyroxine and triiodothyronine during levothyroxine sodium replacement therapy for hypothyroidism. Low levels of biologic markers characteristic of hypothyroidism were normalized in consequence of hormone replacement and a negative correlation between their serum levels and TSH concentration was demonstrated in most subjects. However, in some patients a discrepancy in the response to levothyroxine between the pituitary and other target organs could be revealed. Additional evidence was disclosed that the pituitary thyrotroph sensitizes a minor decrease in serum thyroxine level, which would not be recognized by other target organs. Furthermore, it was revealed that during L-T4 replacement therapy in a large fraction of patients with subnormal serum TSH concentration blood levels of the measured markers often exceeded the upper limit of the normal range indicating the possibility of "tissue"-thyrotoxicosis beside the pituitary in other target organs, too. According to the present study which takes into consideration markers reflecting end-organ responsiveness to thyroid hormones it is recommended to adjust the dose of levothyroxine to maintain serum TSH in the normal range. For patients with subnormal TSH concentration a close follow-up is obligatory and in case od concomitantly raised free-thyroxine level the reduction of the levothyroxine dosage is proposed.

Adult↗
PubMed1992

Disinfection of regenerated dialyzers with ozone.

Regenerated, ozone-disinfected dialyzers were use for a total of 190 hemodialyses in 35 patients without the occurrence of any complication or pyrogen reactions; the efficiency of hemodialysis was identical with that of new or formalin-disinfected dialyzers. The priming fluid contained in both compartments of ozone-disinfected dialyzers proved to be sterile. The ozone did not damage the dialyzer membrane or the supporting structure of dialyzer. Ozone can effectively substitute formalin and other chemical sterilants as a disinfectant of regenerated capillary dialyzers. The process can also be automated.

Blood Cell Count↗
PubMed1992

Radioimmunoassay of thyrotropin-releasing hormone in euthyroid subjects and in patients with thyroid diseases.

An episodic pattern of fluctuation in serum TRH level was demonstrated in euthyroid males. It is suggested that these rises in serum TRH-IR are due to its pulsatile hypothalamic release, however, it must be remembered that a high proportion of circulating TRH is derived from extrahypothalamic sources. The conception that the TSH surge during late evening is secondary to an increased TRH release could not be proved in the present study. In the euthyroid and primary hypothyroid groups the mean values of serum TRH-IR did not differ, while its level was slightly, but not significantly lower in hyperthyroid patients. The evaluation of a single TRH determination is impeded by the short half-life, low serum level and its production in extrahypothalamic sites, too.

Circadian Rhythm↗
PubMed1991

[Biological markers reflecting peripheral effects of thyroid hormones in autonomous thyroid adenoma].

In some patients with functioning thyroid autonomous nodules preclinical hyperthyroidism is detected. It is important to know, whether in this intermediate clinical state beside the suppression of pituitary TSH secretion other target organs are also affected by serum free-thyroxine and free-triiodothyronine levels still within the normal range. Determining some sensitive, but not specific biologic markers reflecting the impact of thyroid hormones at the peripheral tissue level, it was demonstrated that in the group of preclinical hyperthyroidism the mean level of plasma fibronectin exceeded that of the controls (mean +/- S. D.: 583.5 +/- 163.9 vs. 424.2 +/- 84.1 micrograms/ml, p less than 0.001), serum procollagen-III-peptide concentration was already significantly raised, though its value was still within the normal range (mean +/- S. D.: 0.73 +/- 0.17 vs. 0.57 +/- 0.16 U/ml, p less than 0.05), conversely, mean sex-hormone binding globulin level was the same as in euthyroid controls (mean +/- S. D. 47.4 +/- 18.2 vs. 48.3 +/- 16.3 nmol/l). The value of all three parameters was significantly elevated in patients with toxic nodular goiter. Based on the results of this study "tissue"-thyrotoxicosis is suspected in some patients with preclinical hyperthyroidism, which may have therapeutical implications.

Adult↗
PubMed1991

[Role of Chlamydia trachomatis in diseases of the urogenital system].

The authors carried out 402 examinations to detect Chlamydia trachomatis. The agent was detected by serological methods. The cultivation and serological methods proved to give significant results. Doxycyclin and Eryc capsules were successful in the therapy. The great number of positive chlamydia cases suggest to focus attention on detecting the agent on the therapy and on the prevention of the spreading of infection.

Chlamydia Infections↗
PubMed1991

Inactivation of metronidazole by Enterococcus faecalis.

The in-vitro inactivation of metronidazole by different clinical isolates of Enterococcus faecalis was investigated by means of association experiments in which Ent. faecalis strains and Bacteroides fragilis group strains were cultured in the same liquid medium. All of the tested Ent. faecalis strains (20 isolates) were able to protect the B. fragilis group strains against the killing effect of metronidazole at a concentration four or eight times higher than the normal MIC. Different strains of Streptococcus (14), Staphylococcus aureus (10), Staph, epidermidis (10) and Escherichia coli (8) failed to exhibit the same effect. When Ent. faecalis strains were cultured anaerobically for 24 h in the presence of 4 mg metronidazole/l, either alone or together with different B. fragilis group strains, no metronidazole could be detected subsequently in the culture supernatants by HPLC. Concomitantly an increase of four or five logs in the viable counts of the co-cultured Bacteroides strains was observed compared with Bacteroides strains cultured alone. Sonicated cell extracts of Ent. faecalis cultured either aerobically or anaerobically were found to inactivate metronidazole to the same extent, whereas the culture supernatants had no such effect.

Bacteriological Techniques↗
PubMed1991

Electron microscopic investigation of lysogeny of Clostridium difficile strains isolated from antibiotic-associated diarrhea cases and from healthy carriers.

An electron microscopic investigation was performed on 28 Clostridium difficile strains isolated from 15 antibiotic-associated diarrhea cases and from 13 healthy infants. Through the use of supernatants of the cultures induced by mitomycin C (1 or 3 micrograms/ml), 18 of the 28 C. difficile strains proved to harbor phage particles with a different morphology and size. Most of the phages revealed belonged in phage group B1 with a non-contractile tail. Seven of the 11 toxin-producing, lysogenic C. difficile strains carried a defective phage structure (120 nm-long tail with an incomplete head capsule) alone or together with other normal phages. With different C. difficile strains as indicator, plaque formation could not be detected in any of the lysates.

Anti-Bacterial Agents↗
PubMed1991

Biologic blood markers reflecting thyroid hormone effect at peripheral tissue level in patients receiving levothyroxine replacement for hypothyroidism.

Plasma fibronectin, serum procollagen-III-peptide and sex-hormone-binding globulin as non-specific markers of thyroid hormone effect at peripheral tissue level were determined and their values were related with serum levels of TSH, free-thyroxine and triiodothyronine during levothyroxine sodium replacement therapy for hypothyroidism. Low levels of biologic markers characteristic of hypothyroidism were normalized in consequence of hormone replacement and a negative correlation between their serum levels, and TSH concentration was demonstrated in most subjects. However, in some patients a discrepancy in the response to levothyroxine between the pituitary and other target organs was revealed. Additional evidence was disclosed that the pituitary thyrotroph sensitizes a minor decrease in serum thyroxine level, which would not be recognized by other target organs. Furthermore, it was revealed that during L-T4 replacement therapy in a large fraction of patients with subnormal serum TSH concentration blood levels of the measured markers often exceeded the upper limit of the normal range indicating a possibility of "tissue" thyrotoxicosis, besides the pituitary, in other target organs, too. According to the present study, which takes into consideration markers reflecting end-organ responsiveness to thyroid hormones, it is recommended to adjust the dose of levothyroxine to maintain serum TSH in the normal range. For patients with subnormal TSH concentration a close follow-up is obligatory and in case of concomitantly raised free-thyroxine level the reduction of the levothyroxine dosage is proposed.

Adult↗
PubMed1990

[Plasma fibronectin level in thyroid disease].

Fibronectins are widespread components of connective tissues and their production is partly under hormonal control. A study was undertaken to reveal the influence of thyroid diseases on plasma fibronectin level. It is stated that compared with a euthyroid control group (n = 33; 436 +/- 82 micrograms/ml) plasma fibronectin content is significantly higher in untreated, overt hyperthyroidism (n = 27; 1036 +/- 389 micrograms/ml), while its plasma level is lower in hypothyroidism (n = 18; 230 +/- 123 micrograms/ml). Data hint to the possibility that the measurement of plasma fibronectin level may yield a help to assess the effect of thyroid hormones at target organ (connective tissue) level.

Adult↗
PubMed1990

[Serum sex hormone-binding globulin levels in thyroid diseases].

Synthesis of "sex-hormone binding globulin" is influenced by the thyroid hormones and its concentration in the serum may be a marker of the thyroid hormone effect at the peripheral tissue (liver) level. Compared to euthyroid controls serum "sex-hormone binding globulin" concentration is elevated in overt hyperthyroidism (141.6 +/- 37.6 vs 48.3 +/- 16.2 nmol/l; p less than 0.001), conversely, its mean level is decreased in the hypothyroid group of patients (24.9 +/- 14.8 vs 48.3 +/- 16.2; p less than 0.001). In the group of subclinical hyperthyroidism the mean value of "sex-hormone binding globulin" corresponds to that in control subjects (47.4 +/- 16.8), while its serum level is near the lower border of the normal range in subclinical hypothyroidism (33.6 +/- 6.1 vs 48.3 +/- 16.2; p less than 0.01). During thyroid hormone replacement for hypothyroidism measurement of serum "sex-hormone binding globulin" may help to assess the response of the target organs to the hormone therapy. In patients with peripheral thyroid hormone resistance serum "sex-hormone binding globulin" level is within the normal range (51.3 +/- 9.8), its determination supports the diagnosis of this disease.

Adult↗
PubMed1990

Plasmid analysis of clinical isolates of Bacteroides fragilis group strains.

Cryptic plasmids were isolated from 26 of 52 Bacteroides fragilis group strains derived from severe infections. Four strains harboured two plasmids, one three plasmids and one five plasmids with different molecular weights. The same molecular weight plasmid (3.7 Md) was isolated from 17 of the 26 plasmid-containing B. fragilis group strains. No correlation was found between plasmid-harbouring and resistance against ten antibiotics and different heavy metal ions. No curing of the strains from the plasmids was achieved with ethidium bromide and acridine orange.

Anti-Bacterial Agents↗
PubMed1990

Plasma fibronectin level in patients with functional thyroid adenoma.

1. Plasma fibronectin level may serve as a sensitive, but not specific marker of thyroid hormone effect at peripheral, connective tissue level. 2. In some patients with preclinical hyperthyroidism an inverse relationship is detected between pFn and serum TSH reflecting an excessive effect of thyroid hormones at the target organ level. This finding suggests that suppressed TSH secretion might be deleterious in the long-term, which may have therapeutical implications.

Adenoma↗
PubMed1990

[Subclinical hypothyroidism].

Subclinical hypothyroidism was detected in 5.7% of 853 patients referred for ambulatory examination to a department in Prague. In patients referred with a mild degree of subclinical hypothyroidism with an evaluated level of thyrotropic hormone in serum (TSH) no significant deviations of serum thyroxine and triiodothyronine (T4 and T3) were found nor different values of the Achilles tendon reflex time, as compared with patients with normal thyroid function. In case of a more marked grade of subclinical hypothyroidism with an elevated TSH level without stimulation the patients have on average significantly lower T4 vales, significantly longer Achilles tendon reflex times and preejection stage of left ventricular contraction, and of Rodbard's Q-Kd interval (Q-Kd) than euthyroid patients. The values are, however, still within the reference range for normal function and in individual cases cannot be decisive for establishment of the diagnosis. The authors discuss the differential diagnosis and views on treatment of this disease.

Adult↗
PubMed1990

The measurement of the serum sex-hormone binding globulin in various thyroid diseases.

Synthesis of "sex-hormone binding globulin" (SHBG) is influenced by thyroid hormones and its concentration in the serum of female subjects may be a marker of thyroid hormone effect at the peripheral tissue (liver) level. Compared to the levels found in euthyroid females (n = 46), the mean (+/- S.D.) serum SHBG concentration was found elevated in overt hyperthyroidism (Graves' disease: n = 56; 141.6 +/- 37.6 vs. 48.3 +/- 16.2; toxic nodular goiter: n = 16; 119.9 +/- 50.7 vs. 48.3 +/- 16.2 nmol/l; P less than 0.001). In contrast, it was decreased in manifest hypothyroidism (n = 25; 24.9 +/- 14.8 vs. 48.3 +/- 16.2; P less than 0.001). In the group of preclinical hyperthyroidism (n = 43), despite suppressed TSH secretion, the serum value of SHBG was normal (47.4 +/- 16.8), while its serum level approached the lower border of the normal range in subclinical hypothyroidism (n = 10; 33.6 +/- 6.1 vs 48.3 +/- 16.2 nmol/l; P less than 0.01). Data indicate that the pituitary responds more sensitively than the liver to a slight change of the serum thyroid hormone level. During thyroid hormone replacement for hypothyroidism, measurement of serum SHBG may provide help to assess the response of the target organ to the given therapy. In patients with generalized resistance to thyroid hormone, the serum SHBG level is within the normal range (51.3 +/- 9.8 nmol/l), thus, its determination supports the diagnosis of this disease.

Biomarkers↗
PubMed1989

[Thyroid function in severe non-thyroidal diseases].

The aim of the present study was to find out whether a change in the function of the pituitary-thyroid axis can be revealed in a relatively homogenous group of hematological patients. To clarify this problem serum levels of total-thyroxine and triidothyronine, free-thyroxine and free-triiodothyronine, reverse-triidothyronine and thyrotropin were detected in these patients. The majority of subjects with chronic myelogenous leukemia (in the remission phase) have normal pituitary-thyroid function, however a change in the peripheral metabolism of thyroxine can be revealed. Longitudinal studies in patients with acute myelogenous leukemia indicate that in some cases with the progression of the disease serum TSH and thyroid hormone levels decrease referring to secondary hypothyroidism and in these cases the measurement of serum free-thyroxine content by an analogue tracer method is not recommended. On the basis of the investigational results it is stated that in hematological patients the pituitary-thyroid function is influenced by the phase of illness and by the results of the given treatment.

Humans↗
PubMed1989

Androgens and bone mineral content in patients with subtotal thyroidectomy for benign nodular disease.

To investigate the influence of thyroid surgery on the skeleton and different hormones a well characterized patient group of 24 women was selected who had undergone subtotal thyroidectomy for euthyroid benign nodular disease and remained euthyroid after the operation. Bone mineral content was determined in lumbar vertebrae, femoral neck and radius by dual and single photon absorptiometry. The serum levels of calcitonin, dehydroepiandrosterone, dehydroepiandrosterone-sulphate, androstenedione, total testosterone, cortisol and 25-hydroxyvitamin D were measured. A control group was created of 48 healthy female subjects. No reduction in bone mass was observed at the measured sites compared to appropriate controls. Beside normal bone mineral content significantly elevated serum levels of dehydroepiandrosterone (27.4 +/- 10.4 vs. 20.8 +/- 6.9 nmol/l) and androstenedione 9.3 +/- 3.3 vs. 6.6 +/- 2.2 nmol/l) were found without any clinical sign of androgen excess. There was no correlation between bone mineral content and these androgen levels. The serum calcitonin levels of all patients were low. With regard to the previously reported interactions among androgen, calcitonin and bone metabolism, our results raise the possibility of a relationship between higher androgen levels and preserved bone mass in these patients, while normal bone mineral content and calcitonin deficiency in these patients does not inevitably indicate that calcitonin does not affect bone tissue in adults.

Adenoma↗
PubMed1989

Thyroid function in severe "nonthyroidal illness". Longitudinal studies in haematological patients.

It is known that in severe nonthyroidal illness the regulation of thyroid function, the distribution and metabolism of thyroid hormones may change. The present study aimed at clarifying whether a change in the function of the pituitary-thyroid axis can be detected in an approximately homogeneous group of haematological patients, and how it is correlated with the various phases of the disease and with the therapeutic result. Studies were performed on patients with chronic and acute myelogenous leukaemia: serum levels of total thyroxine and triiodothyronine, free thyroxine and triiodothyronine, reverse triiodothyronine and thyrotropic hormone were determined. Apart from a few cases, there was no dysfunction of the pituitary-thyroid axis in chronic leukaemic patients being in the remission phase. However, the peripheral thyroxine metabolism may be altered. The longitudinal studies on acute myelogenous leukaemic patients indicate that, with the progression of the disease, serum TSH and thyroid hormone levels were reduced in a part of the cases and it is not justified to assess the free serum thyroxine level by an analogue-tracer method in this disease. The examinations have revealed that the various phases of the clinical picture as well as the therapeutic results considerably influence the function of the pituitary-thyroid axis. It seems reasonable to consider these findings in the other severe nonthyroidal illnesses as well.

Adult↗