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Biomedical subjects

J F Scott

Publications and source records attributed to J F Scott.

At least 37 records · Page 2Linked to original sources
PubMed1993

The Canadian Palliative Care Undergraduate Curriculum.

In 1988 the deans of 12 (out of 16) Canadian medical schools appointed representatives to a Canadian Palliative Care Curriculum Committee. The Committee has subsequently published the Canadian Palliative Care Curriculum which outlines specific goals and objectives for palliative care instruction in undergraduate medical teaching. The Curriculum covers 22 different topics, including 13 symptoms and 9 psychosocial issues. The Canadian Curriculum Committee continues to meet and is now considering other national educational initiatives in palliative care.

Canada↗
PubMed1992

Primary care providers' perceptions of care.

The Regional Palliative Care Unit in Ottawa conducted a retrospective study to determine how satisfied patients and families were with the service the Unit offers. Forty-five primary care providers, bereaved from 6 to 12 months, completed the 64-item semistructured telephone interview in a mean time of 20 minutes. The 59 closed-ended questions rated satisfaction levels concerning various aspects of the palliative care service on a 5-point Likert scale. Five open-ended questions elicited family priorities and suggestions for improvement. The major finding was that care was perceived to be highly satisfactory. Unexpected outcomes of the study were the identification by family members of the criteria for a "good death" and families' perceptions that the Unit offers the best quality of life and death even for those patients who resist admission to the hospice setting.

Family↗
PubMed1988

Anti-fluorescein antibody 3-13 VH gene rearrangement in idiotypically cross-reactive hybridomas.

The deduced amino acid sequence of anti-fluorescein (F1) antibody 3-13 VH region (residues 1-95) was 78% homologous to the alpha-1----3-dextran binding myeloma protein J558 VH region and was in the Wu-Kabat Subgroup II or Dildrop Group I classifications. The 3-13 VH region was rearranged to a D segment with only 8 of 30 bp in common with DFL16.1 germ line D gene and less homologous to all other previously identified D sequences. This sequence was joined to the third codon of JH4. The sequence encoding VH residues 5-91 was subcloned into pSP65 and used as a probe in Southern analyses to monitor 3-13 VH gene rearrangements in 12 other anti-F1 hybridomas differentially expressing (or not at all) the 3-13 idiotype. Three clones which inhibited the 3-13 idiotype-anti-idiotype interaction as effectively as 3-13 (3-12, 3-17 and 3-35), all had rearranged a gene which hybridized to the cloned 3-13 fragment, however, each was contained on a different size restriction fragment. Analyses of five other idiotypically related (but not identical) hybridomas indicated that four had rearranged a cross hybridizing VH gene while no such rearrangements were detected among four idiotypically negative cell lines. A restriction site assay indicated five clones examined had all rearranged a Vk gene to the Jk1 or Jk2 gene segment. The sequence of the antibody 3-13 VH gene and its use in hybridization studies represent the first molecular analysis of a recurrently expressed repertoire specific idiotype within an unrestricted immune response.

Amino Acid Sequence↗
PubMed1986

Mutations in ARS1 increase the rate of simple loss of plasmids in Saccharomyces cerevisiae.

Autonomously replicating sequence (ARS) elements are DNA sequences that promote extrachromosomal maintenance of plasmids in yeast. Mutations generated in vitro in the ARS1 region were examined for their effect on plasmid maintenance in a yeast centromeric plasmid. Our data show that mutations in the regions surrounding the ARS1 consensus sequence cause increases in the frequency of simple loss (1:0) events without affecting the rate of nondisjunction (2:0). Removal of the consensus sequence itself causes a drastic increase in the rate of simple loss. Sequences sensitive to mutagenesis were identified in each flanking region and differ with respect to their location and importance to ARS function. These results suggest that the role ARS1 plays in plasmid maintenance deals with the replication and/or localization of the plasmid in yeast.

Centromere↗
PubMed1985

DNA synthesis in yeast cell-free extracts dependent on recombinant DNA plasmids purified from Escherichia coli.

In our attempts to establish a cell-free DNA replication system for the yeast Saccharomyces cerevisiae, we have observed that recombinant DNA plasmids purified from Escherichia coli by a common procedure (lysozyme-detergent lysis and equilibrium banding in cesium chloride ethidium bromide gradients) often serve as templates for DNA synthesis by elongation enzymes. The templates could be elongated equally well by enzymes present in the yeast cell-free extracts, by the large proteolytic fragment of E. coli DNA polymerase I or by T4 DNA polymerase. The template activity of the purified plasmids was dependent on the presence of heterologous DNA segments in the bacterial vectors. The template activity could be diminished by treatment with alkali. We propose that the ability of recombinant plasmids isolated from bacterial hosts to serve as elongation templates may lead to erroneous conclusions when these plasmids are used as templates for in vitro replication or transcription reactions.

Cell-Free System↗
PubMed1985

New vectors for construction of recombinant high-copy-number yeast acentric-ring plasmids.

Yeast acentric-ring plasmid 1 (YARp1), comprising 1453 bp of entirely yeast chromosomal DNA, is maintained in Saccharomyces cerevisiae as a high-copy, relatively stable plasmid. To determine the feasibility of using YARp1 as a yeast cloning vehicle, we subcloned the GAL1-10 promoter and the URA3 gene into YARp1 at different locations. To facilitate these constructions, a class of permuted YARp1 construction vectors was generated which enabled us to use various restriction sites in YARp1 as insertion points. Transformation frequencies, plasmid stabilities, and copy numbers of these YARp1 derivatives remained elevated, comparable to those of YARp1 itself. Also, when OMP decarboxylase was assayed using strains containing URA3-YARp's, specific activities of 100-300 times that of wild type were found. This evidence supports the use of YARp1 as a high-copy yeast-expression vector or for analyzing structural and regulatory DNA sequences.

Base Sequence↗
PubMed1985

Alternative model for chromatin organization of the Saccharomyces cerevisiae chromosomal DNA plasmid TRP1 RI circle (YARp1).

TRP1 RI circle (now designated YARp1, yeast acentric ring plasmid 1) is a 1,453-base-pair artificial plasmid composed exclusively of Saccharomyces cerevisiae chromosomal DNA. It contains both the TRP1 gene and ARS1 (a DNA sequence that permits extrachromosomal maintenance of recombinant plasmids). This high-copy-number, relatively stable plasmid was shown to be organized into nucleosomes comparable to typical yeast chromatin, containing a possible maximum of nine nucleosomes per circle. Therefore, YARp1 can be used to examine the structure of chromatin of both a chromosomally derived replicator and a functional gene. By mapping regions of micrococcal nuclease cleavage in chromatin versus purified DNA, we located the positions of protected regions on the circle with reference to six unique restriction sites. Measurements made on patterns of early digestion products indicated that a region of approximately 300 base pairs in the vicinity of ARS1 was strongly resistant to micrococcal nuclease. The remainder of the plasmid appeared to be associated with five positioned nucleosomes and two nonnucleosomal, partially protected regions on the bulk of the molecules. After similar extents of digestion, naked DNA did not exhibit an equivalent pattern, although some hypersensitive cleavage sites matched sites found in the chromatin. These results are consistent with the interpretation that the protected domains are aligned with respect to a specific site or sites on the small circular chromatin.

Base Composition↗