Biomedical subjects

J F Greenleaf

Publications and source records attributed to J F Greenleaf.

At least 37 records · Page 2Linked to original sources

PubMed2000

Three-dimensional optical measurement of instantaneous pressure.

Local perturbations in material density induced in a material by a compressional wave give rise to local perturbations in refractive index. Accurate, high-resolution, three-dimensional, optical measurements of an instantaneous refractive index perturbation in a homogeneous, optically transparent medium may be obtained from measurements of scattered optical intensity alone. The method of generalized projections allows incorporation of optical intensity measurements into an iterative algorithm for computing the phase of the interrogating optical pulse as the solution of a fixed point equation. The complex optical field amplitude, computed in this manner, is unique up to a constant unit magnitude complex coefficient. The three-dimensional refractive index distribution may be computed via the Fourier slice reconstruction algorithm from the optical phase data under the assumption of weak optical scattering. The refractive index perturbation is related to local instantaneous pressure under a linear, small-displacement model for the mechanical wave. A numerical simulation of the measurement experiment, phase recovery, and reconstruction process for a plane piston ultrasound transducer with a semicircular aperture and center frequency of 1.5 MHz is described and corresponds very well with experiment. Experimental data obtained using an 810-nm laser source are used to reconstruct the three-dimensional pressure field from two elements of a 2.5-MHz linear array. Comparison with a measurement obtained via a 500-microm needle hydrophone shows excellent agreement.

Acoustics↗

PubMed2000

Tissue harmonic imaging: experimental analysis of the mechanism of image improvement.

Tissue harmonic scanning visually improves echocardiographic image quality. The aim of the present study was to objectively assess the improvement in harmonic image quality under controlled laboratory conditions. A tissue-mimicking phantom that contained 8-mm-diameter cystic lesions at depths ranging from 2 to 12 cm was used. Harmonic scans (1.7 MHz transmit, 3.4 MHz receive) of the phantom were obtained and lesion detectability was compared to that in scans acquired with 2 fundamental frequencies (2.0 and 3.3 MHz). A 2 cm-thick ethanol layer was also used to simulate the nonlinear effect of human fat. Cyst detectability was quantified by measurement of the contrast-to-speckle ratio (CSR). The results indicated no significant difference in the CSR between harmonic and fundamental images obtained without the ethanol layer. With images obtained with the ethanol layer, a relative increase of the CSR during harmonic imaging was observed with respect to fundamental imaging (p<0.05). In conclusion, a fat layer, here simulated by ethanol, plays a significant role in determining the resulting image quality. Without this layer, the contribution of the second harmonic mode was not significant. Thus, in a slim patient, the harmonic mode may not be as beneficial to image improvement as in an obese patient.

Echocardiography↗

PubMed2000

Myocardial contraction maps using tissue Doppler acceleration imaging.

OBJECTIVE: To evaluate the tissue Doppler acceleration imaging (TDAI) data which can be used to determine the intramural site of origin of myocardial contraction in response to electrical stimulation. METHODS: Six open-chest pigs with left ventricle (LV) pacing were evaluated with TDAI. An epicardial surface scanning method was used to collect short-axis views of the left ventricle. The electrode was implanted from the epicardium through the anterior free wall to an intramural position. RESULTS: During pacing, the intramural onset of myocardial acceleration occurred within 33 ms after electrical stimulation and always surrounded the embedded subendocardial end of the pacing needle. The observed short-axis diameter of the area of initial myocardial acceleration ranged from 2.9 mm to 5.0 mm (4.2 +/- 0.9 mm, n = 6). The onset of myocardial acceleration allowed appreciation of the initial intramural myocardial contraction. The spatial size and acceleration magnitude of the initial myocardial acceleration distribution were irregular. CONCLUSION: Two-dimensional myocardial acceleration mapping can show the intramural site of origin of myocardial contraction in response to paced electrical stimulation. The location of myocardial acceleration conformed to the site of initial electrical stimulation. The delay to the earliest regional myocardial contraction, 33 ms after paced electrical stimulation, was related to the frame rate of image acquisition.

Animals↗

PubMed1999

Vibro-acoustography: an imaging modality based on ultrasound-stimulated acoustic emission.

We describe theoretical principles of an imaging modality that uses the acoustic response of an object to a highly localized dynamic radiation force of an ultrasound field. In this method, named ultrasound-stimulated vibro-acoustography (USVA), ultrasound is used to exert a low-frequency (in kHz range) force on the object. In response, a portion of the object vibrates sinusoidally in a pattern determined by its viscoelastic properties. The acoustic emission field resulting from object vibration is detected and used to form an image that represents both the ultrasonic and low-frequency (kHz range) mechanical characteristics of the object. We report the relation between the emitted acoustic field and the incident ultrasonic pressure field in terms of object parameters. Also, we present the point-spread function of the imaging system. The experimental images in this report have a resolution of about 700 microm, high contrast, and high signal-to-noise ratio. USVA is sensitive enough to detect object motions on the order of nanometers. Possible applications include medical imaging and material evaluation.

Journal Article↗

PubMed1999

Low-intensity ultrasound stimulates proteoglycan synthesis in rat chondrocytes by increasing aggrecan gene expression.

We evaluated the effect of low intensity-pulsed ultrasound stimulation on rat chondrocytes in vitro using two different 1.0-MHz ultrasound signals with spatial and temporal average intensities of 50 or 120 mW/cm2. The pulses had a duration of 200 microseconds and were repeated every millisecond, with corresponding average peak-pressure amplitudes of 230 or 360 kPa, respectively. Cells were stimulated one, three, or five times for 10 minutes each day starting the third day after plating. One group of cells was exposed to sham ultrasound as a control. The cultures were evaluated for cell proliferation (by [3H]thymidine incorporation and DNA measurement), steady-state mRNA levels of alpha1(I) and alpha1(II) procollagens and aggrecan (by Northern blotting), and proteoglycan synthesis (by [35S]sulfate incorporation). The results revealed that ultrasound causes increases in the level of aggrecan mRNA (p < 0.05) and in proteoglycan synthesis (p < 0.03) after three and five treatments. Expression of mRNA for alpha1(II) procollagen increased over time, but ultrasound had no stimulatory effect. Expression of mRNA for alpha1(I) procollagen was initially low and remained unchanged with time. Although cell proliferation increased with time in both groups, there was no statistically significant difference between the cultures treated with ultrasound and the controls (p = 0.1). The in vitro results support our previous in vivo findings that low-intensity ultrasound stimulates aggrecan mRNA expression and proteoglycan synthesis by chondrocytes, which may explain the role of ultrasound in advancing endochondral ossification, increasing the mechanical strength of fractures, and facilitating fracture repair.

Aggrecans↗

PubMed1999

Application of radiation force in noncontact measurement of the elastic parameters.

Ultrasound-stimulated vibro-acoustic spectrography is a recently-developed method that employs the radiation force of two intersecting continuous ultrasound beams to remotely vibrate an object at an arbitrary low frequency. Object vibration produces a sound field (acoustic emission) in the medium, which is a function of object mechanical properties. By measuring the acoustic emission field, one can obtain information about the mechanical parameters of the object. In this paper, we use this method for remote (noncontact) measurement of the dynamic Young's modulus of a rod based on its fundamental resonance frequency. Experimental results on an aluminum rod agree with the published data.

Elasticity↗

PubMed1999

Evolving era of multidimensional medical imaging.

Currently, computer-assisted imaging can visualize very fast or very slow nonvisible motion events. We can create measurable geometric representations of physiology, including transformation, blood flow velocity, perfusion, pressure, contractility, image features, electricity, metabolism, and a vast number of other constantly changing parameters. The greatest attribute is the ability to present physiologic phenomena as easily understood geometric images more suited to the human's four-dimensional comprehension of reality. The key research challenges are to discover new visual metaphors for representing information, understand the analysis tasks that they support, and associate relevant information to create new information.

Diagnostic Imaging↗

PubMed1998

Ultrasound-stimulated vibro-acoustic spectrography.

An ultrasound method based on radiation force is presented for imaging the acoustic response of a material to mechanical excitation. Acoustic energy was emitted from solids and tissues in response to an oscillatory radiation force produced by interfering focused beams of ultrasound. Frequency spectra of ultrasound-stimulated acoustic emission exhibited object resonances. Raster-scanning the radiation force over the object and recording the amplitude and phase of the emitted sound resulted in data from which images related to the elastic compositions of the acoustically emitting objects could be computed. Acoustic emission signals distinguished tuning-fork resonances, submillimeter glass spheres, and calcification in excised arteries and detected object motions on the order of nanometers.

Acoustic Stimulation↗

PubMed1998

Artificial cavitation nuclei significantly enhance acoustically induced cell transfection.

The efficiency of ultrasound-mediated gene transfection was enhanced three- to fourfold, compared to previous results, through the use of green fluorescent protein reporter gene, cultured immortalized human chondrocytes and artificial cavitation nuclei in the form of Albunex. Cells were exposed to 1.0-MHz ultrasound transmitted through the bottom of six-well culture plates containing immortalized chondrocytes, media, DNA at a concentration of 40 micrograms/mL and Albunex at 50 x 10(6) bubbles/mL. Transfection efficiency increased linearly with ultrasound exposure pressure with a transfection threshold observed at a spatial average peak positive pressure (SAPP) of 0.12 MPa and reaching about 50% of the living cells when exposed to 0.41 MPa SAPP for 20 s. Adding fresh Albunex at 50 x 10(6) bubbles/mL prior to sequential 1-s, 0.32- or 0.41-MPa exposures increased transfection with each exposure, reaching 43% transfection after four exposures. Efficient in vitro and in vivo transfection now appear possible with these enhancements.

Albumins↗

PubMed1998

Image enhancement by noncontrast harmonic echocardiography. Part II. Quantitative assessment with use of contrast-to-speckle ratio.

OBJECTIVE: To ascertain whether "harmonic imaging"--use of ultrasound signals with the frequency twice that of the transmitted signal for ultrasound image generation--can improve image contrast while reducing noise. METHODS: Technically difficult echocardiograms (nonvisualization of 2 or more endocardial segments in a 16-segment model) from 25 patients were analyzed. Corresponding fundamental and harmonic images of the left ventricle in the apical four-chamber, two-chamber, and long-axis views were divided into basal, mid, and apical regions. The difference in image quality between fundamental and harmonic scans was assessed by using the muscle-to-cavity contrast-to-speckle ratio (CSRmc). RESULTS: The mean CSRmc values of pooled data revealed significant image enhancement by harmonic scanning (CSRmc increased from 0.84 to 1.06; P < 0.0001). Regression analysis showed that harmonic imaging improved the CSRmc values in 68% of all scans. Regional analysis indicated the most enhancement in basal regions (CSRmc increased from 0.96 to 1.34; P < 0.0001), followed by the mid (CSRmc increased from 0.84 to 1.04; P < 0.0001) and apical (CSRmc increased from 0.68 to 0.74; P = 0.0138) left ventricular regions. CONCLUSION: Noncontrast harmonic imaging significantly enhances suboptimal echocardiographic images, particularly in the regions distant from the transducer.

Adult↗

PubMed1997

Toroidal geometry: novel three-dimensional intracardiac imaging with a phased-array transducer.

Recent advances in small, linear-array transducers have opened new avenues for three-dimensional image acquisition from an intracardiac approach. The purpose of this study was to introduce a novel method of image acquisition using toroidal geometry, explore its fidelity of reproduction of three-dimensional cardiac anatomy, and determine whether a whole-heart scan is achievable. Acquisition was accomplished through 360-degree incremental rotation of a rigid endoscope with a side-mounted ultrasound transducer. The procedure was first tested with the use of a gelatin model to define far-field slice resolution with 1.8-degree rotational increments. Comparison of three-dimensional scans of cardiac specimens with corresponding photographs confirmed that toroidal geometry can provide a high-quality display of structures from all sides. We conclude that whole-heart three-dimensional scanning from within the cardiac chambers is possible with toroidal geometry. The quality of depicted anatomy depends on transducer location within the heart, distance from the transducer, density of slices, and image resolution. The potential of intracardiac three-dimensional ultrasound imaging includes detailed spatial evaluation of cardiac morphology, determination of appropriate placement of investigative or therapeutic devices (catheters, closure devices, etc.), and assessment of cardiac function.

Animals↗

PubMed1997

Detection of cardiac boundaries in echocardiographic images using a customized order statistics filter.

Order statistics filters are nonlinear filters that suppress impulsive and Gaussian noise while preserving edges. These features are particularly useful for cardiac boundary detection in ultrasound images. Based on these facts, we have analyzed performance of a combined ranked order statistics filter. The filter subtracts ranks of ordered highest and lowest intensity values of pixels encompassed in a filter window. The rank extent and window size selection allow adjustment of filter properties for a particular application. Increasing the rank fosters the low-pass characteristics of the filter. Increasing the window size supports noise removal but reduces anatomic selectivity. The filter highlights cardiac boundaries in clinical echocardiograms with intensity proportional to the local probability of a presence of the boundary.

Echocardiography↗

PubMed1996

Ultrasound-mediated transfection of mammalian cells.

Mammalian cells were successfully transfected with plasmid DNA in vitro using ultrasound transmitted through the walls of cell culture flasks or plates. Primary rat fibroblasts or chondrocytes were exposed to ultrasound in the presence of plasmids containing lacZ or neo genes. The transfection efficiency was evaluated by counting the number of beta-galactosidase (beta-Gal) positive cells or neomycin-resistant colonies. Transfection efficiency was optimized by varying ultrasound conditions, ambient temperatures (room temperature or 37 degrees C), plasmid concentrations, and initial cell populations. Additional experiments were performed performed to elucidate the mechanism of the ultrasound-mediated transfection. Maximal gene transfection was seen with two ultrasound conditions: 1-MHz carrier frequency 411 +/- 189 kPascal continuous wave with 20 or 30 sec of exposure time, and 1 MHz carrier frequency 319 +/- 157 kPascal continuous wave with 40 or 60 sec of exposure time. Gene expression was negligible when transfection procedures were performed at room temperature. The average stable transfection rate was 0.34% of surviving cells with a plasmid concentration of 40 micrograms/ml in primary fibroblasts. The transient transfection rate was 2.4% of surviving cells for primary chondrocytes. Data suggest that increasing plasmid concentration will increase efficiency. Identical treatment with 3.5 MHz produced no transfection, implying that cavitation produced by the ultrasound pressure wave appeared to play a critical role in mediating transfection. Ultrasound-mediated transfection was effective for suspended cells as well as for plated cells. This transfection method is simple, easy to keep sterile, and convenient. Ultrasound-mediated transfection appears to be a promising method for gene transfer into mammalian cells.

Animals↗

PubMed1996

Stimulation of secretion from bovine adrenal chromaffin cells by microsecond bursts of therapeutic levels of ultrasound.

1. In this study the secretory response of individual bovine adrenal chromaffin cells was monitored using amperometric carbon-fibre microelectrodes. Cells were stimulated to secrete by exposure to 20-100 microseconds long tonebursts of ultrasound (2-4 x 10(5) Pa; peak pressure at 1 MHz). 2. Three types of secretory responses were observed: an almost instantaneous response, a delayed release of catecholamines, or a series of 'burst-like' secretory bouts. 3. Fura-2 measurements of intracellular Ca2+ concentrations showed that the release of catecholamines was accompanied by an increase in the intracellular Ca2+ concentration. In the absence of extracellular Ca2+, secretory responses were not evoked showing that Ca2+ entry was necessary to elicit catecholamine release.

Adrenal Glands↗

PubMed1996

Exposure to low-intensity ultrasound increases aggrecan gene expression in a rat femur fracture model.

The effects of ultrasound stimulation on various parameters of bone repair after diaphyseal injury were assessed in a standard rat femur fracture model. Bilateral closed femoral fractures were made in 79 skeletally mature male Long-Evans rats. An ultrasound signal consisting of a 200 microsecond burst sine wave of 0.5 MHz repeating at 1 kHz, with an intensity of 50 or 100 mW/cm2 spatial and temporal average, was applied to one fracture in each animal. The contralateral fracture was not exposed to ultrasound and served as a control. Mechanical testing of the healing fracture was performed 3 weeks after injury. In fractures treated with a 50 mW/cm2 ultrasound signal, the average maximum torque (223.5 +/- 50.5 Nmm compared with 172.6 +/- 54.9 Nmm, p = 0.022, paired t test) and average torsional stiffness (13.0 +/- 3.4 Nmm/degree compared with 9.5 +/- 2.9 Nmm/degree, p = 0.017) were significantly greater in treated than in control fractures. In animals treated with a 100 mW/cm2 ultrasound signal, the average maximum torque and torsional stiffness were greater in treated than in control fractures, but this trend did not reach statistical significance. Biochemical analysis of callus in ultrasound-treated and control fractures failed to demonstrate significant differences in cell number, collagen content, or calcium content. Evaluation of gene expression in fractures treated with 50 mW/cm2 ultrasound demonstrated a shift in the expression of genes associated with cartilage formation; aggrecan gene expression was significantly higher on day 7 after fracture and significantly lower on day 21 (p = 0.033 and 0.035, respectively). alpha 1(II) procollagen gene expression was similarly modified, but this trend did not reach statistical significance. Expression of genes coding for bone-related proteins, including alpha 1(I) procollagen, bone gamma-carboxyglutamic acid protein, alkaline phosphatase, and transforming growth factor-beta 1, did not differ between ultrasound-treated and control fractures. These data suggest that ultrasound stimulation increased the mechanical properties of the healing fracture callus by stimulating earlier synthesis of extracellular matrix proteins in cartilage, possibly altering chondrocyte maturation and endochondral bone formation.

Aggrecans↗

PubMed1996

Magnetic resonance imaging of transverse acoustic strain waves.

We describe a phase contrast based MRI technique with high sensitivity to cyclic displacement that is capable of quantitatively imaging acoustic strain waves in tissue-like materials. A formalism for considering gradient waveforms as basis functions to measure arbitrary cyclic motion waveforms is introduced. Experiments with tissue-like agarose gel phantoms show that it is possible to measure small cyclic displacements at a submicron level by an appropriate choice of the applied gradient basis function and to use this capability to observe the spatial and temporal pattern of displacements caused by acoustic strain waves. The propagation characteristics of strain waves are determined by the mechanical properties of the media. It is therefore possible to use this technique to noninvasively estimate material properties such as elastic modulus.

Acoustics↗

PubMed1996

Real-time assessment of the parameter of nonlinearity in tissue using "nonlinear shadowing".

A real-time pulse-echo method for assessing tissue nonlinearity is presented. Here, tissue nonlinearity refers to the parameter of nonlinearity, B/A, and not the frequency dependence of the attenuation. Our nonlinearity assessment method is based on two pulse transmissions per beam position. One pulse is transmitted at a high intensity that propagates nonlinearily and, the other, at a low level, that propagates linearly. Echo data are used to estimate a function representing the decay of fundamental frequency amplitude due to nonlinearity along the ultrasound path. This function is then mapped into an image that includes shadows which relate to the nonlinearity of the preceding regions. To delineate a region of interest from the background tissue, a normalization curve is defined and used to compensate for the nonlinearity of the background tissue. Experiments are performed on a tissue-mimicking phantom and in vitro tissue samples including regions of high nonlinearity. Results indicate that the method can effectively identify nonlinear regions and assess the extent of the nonlinearity.

Adipose Tissue↗

PubMed1996

The nonlinear interaction of two plane waves in a viscous medium.

Earlier studies [P.J. Westervelt, J. Acoust. Soc. Am. 29, 199-203, 934-935 (1957)] of the mutual nonlinear interaction of two plane waves of sound with each other are extended to include the viscous effect. The viscous effect is considered both from the equations of motion and the equation of state of the medium. An analytical solution to the lowest-order scattering process is obtained if the viscous effect of second order and higher can be neglected.

Models, Theoretical↗