Vertebrate host relationships and distribution of ixodid ticks (Acari: Ixodidae) in Connecticut, USA.
Explore the source record for details and available documents.
Biomedical subjects
Publications and source records attributed to J F Anderson.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
A sensitive and reproducible indirect fluorescent antibody test for a North American isolate of Babesia gibsoni was described. In blind tests (coded samples), titer reproducibility was within two 4-fold dilutions and the appropriate positive and negative sera were identified as such. Babesia gibsoni antisera reacted with homologous antigen at one to three 4-fold dilutions greater than with the following heterologous species: B bigemina, B argentina, B equi, Plasmodium falciparum, and P brasilianum. The titer of B canis antiserum to B gibsoni antigen was 1:1,024. low parasitemia (less than 0.01%) of B gibsoni preceded development of minimum significant titer (1:64) by 10 days in a splenectomized dog. Maximum titer was detected 25 days later. In a nonsplenectomized dog, low parasitemia (less than 0.01%) was noted 6 days after injection; it reached 8% at 5 weeks and decreased to and remained below 1% at 7 weeks. A titer of 1:256 occurred at 25 days and increased to 1:65,536 at 18 weeks. Thereafter, it fluctuated to 1:4,096 until the experiment was ended at 24 weeks. organismic antigen retained its reactivity for 5 days at 5 C. This indirect fluorescent antibody test should be useful in the diagnosis of dogs infected with B gibsoni.
Explore the source record for details and available documents.
A domestic dog residing in New England suffered a fatal febrile illness caused by a Babesia infection. The morphology of these intraerythrocytic protozoa and the range of hosts that could be infected experimentally suggested that the parasite was B. gibsoni. Although this tick-bourne disease is enzootic in wild and domestic Canidae in Africa and Asia, it appears to be new to the Americas.
Explore the source record for details and available documents.
Fundamental to maintaining a healthy environment in a mechanically ventilated livestock building is a continuous flow of air, with at least 4 exchanges per hour to remove moisture and aerosol contaminants. A correctly designed fresh air intake system is mandatory for uniform air distribution. Proper insulation is essential. Supplemental heat may be required to permit continuous ventilation while maintaining an ambient temperature suitable for preserving animal health.
Three parameters were used in 1976 and 1977 to assess the status of Rocky Mountain spotted fever (RMSF) in Connecticut--compilation and review of clinical data on suspected human cases for the 13-year period 1965--1977, examination of tick tissues for spotted fever-group rickettsiae by the hemolymph test and direct immunofluorescence, and analyses of mammalian sera for antibodies against Rickettsia rickettsii. There were six presumptive RMSF cases which probably originated in Connecticut. Four of these cases occurred in areas where the American dog tick, Dermacentor variabilis, abounds. A total of 2994 ticks were examined by the hemolymph test. Rickettsia-like organisms were observed in 67 (2.9%) of 2330 D. variabilis and two (0.6%) of 351 Ixodes sp. near scapularis. Fewer than one-half of these organisms stained positively with spotted fever-group conjugate. Microagglutination tests on 1093 mammalian sera indicated that eight (16%) of 49 raccoons, 14 (2.6%) of 549 white-tailed deer, eight (1.7%) of 470 white-footed mice, and one of two gray squirrels had agglutinins in titers greater than or equal to 1:8 against R. rickettsii. Spotted fever-group rickettsiae are present at low frequency in inland as well as coastal regions of Connecticut.
Explore the source record for details and available documents.
A total of 612 Peromyscus leucopus, 11 Microtus pennsylvanicus, 21 Clethrionomys gapperi, and 4 Tamias striatus was collected in Connecticut and examined for Babesia and Grahamella during 1976 and 1977. Babesia antibodies were detected in sera of 9 P. leucopus collected from 4 sites. Babesia parasites were not detected in the blood smears of captured rodents. Subsequent splenectomy and subinoculation of blood from these rodents into susceptible animals failed to induce disease and no Babesia was isolated. Six of 10 P. leucopus inoculated with a Shelter Island, New York strain of B. microti remained infected for 3 1/2 months. Indirect fluorescent antibody titers of experimentally infected P. leucopus ranged from 1:8 to 1:256. Prevalence of Grahamella peromysci infection, as determined from examinations of blood smears of P. leucopus, was 13%. This infection rate is a conservative estimate because parasitemia is difficult to detect in intact animals. Twenty of 58 P. leucopus, taken at 2 sites and with negative blood smears for G. peromysci, developed parasitemia after splenectomy.
Explore the source record for details and available documents.
A modified membrana nictitans flap technique was used successfully in 1,768 of 1,845 (95.8%) cattle with several types of ulcerative keratitis. The flap was easily applied and lasted 7 to 10 days.
A technique for routine hyptonic double-contrast studies of the upper gastrointestinal tract is described. It is emphasized that, to achieve consistent success, the principles of the technique must be understood and applied at the time of the examination. The various factors producing the barium coating effect are described. Gas distension and bubble control are also described. Where applicable, the principles and projections are demonstrated by examples. Endoscopy with biopsy has added a new dimension of accuracy to diagnosis and is replacing barium studies for acute upper gastrointestinal haemorrhage. Accurate barium studies are important as a screening test of other symptomatology to select patients for further clarification by endoscopy.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
In 1970, the College of Veterinary Medicine at the University of Minnesota started an externship program as part of their large animal clinical education program. The basic idea was to work in cooperation with practitioners, using their practice world as a teaching resource. During the 1973-1974 school year, this program was evaluated by the University of Minnesota Measurement Services Center. In this externship program, senior year students spend 1 or 2 weeks in practice that closely matches each student's career interest, eg, cow-calf, dairy, swine, or zoo. The participating veterinarians are charged with the responsibility of performing as teachers during the field experience and of assisting the student in preparing a seminar topic. This seminar topic is presented in a discussion format during the spring quarter to other externship students, faculty, and attending practitioners. Since 1970, a total of 176 students have taken externships, and every year 15 to 20 group practices have participated. The principal evaluation results were based on questionnaire responses from 36 students and 20 practices during the 1973-1974 academic year. Students and practitioners indicated that the externship had been a beneficial experience for them. Many felt that the period of externship should have been longer. In general, practitioners agreed that students were able to manage most diagnostic situations and manipulative procedures that occurred during the externship. The seminar was judged by students and practitioners to be a valuable part of the program, and faculty who participated in the seminar believed that it made them more aware of field situations facing practicing veterinarians.