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Biomedical subjects

J F Adams

Publications and source records attributed to J F Adams.

63 records · Page 4Linked to original sources
PubMed1965

Quantitative aspects of the excretion of radioactive and microbiologically active material in urine after parenteral hydroxocobalamin.

The radioactive material in urine after parenteral radioactive hydroxocobalamin is composed of radioactive cyanocobalamin, radioactive hydroxocobalamin, and a radioactive anionic complex. Studies in vitro suggest that these materials have a similar microbiological activity. Comparison of the amounts of radioactive and microbiologically active material in urine after parenteral radioactive hydroxocobalamin show a significant correlation both in the first and second 24 hours after injection showing that the amount of radioactivity is an acceptable measure of the loss of cobalamin in urine. The slope of the regression lines obtained from cobalamin-deficient and normal subjects implies that there is no equilibration of injected radioactive hydroxocobalamin and body stores: this may be due to the absence of hydroxocobalamin from tissues or to the fact that tissue hydroxocobalamin is bound and incapable of equilibration.

Hydroxocobalamin↗
PubMedDate not supplied

A simple HPLC assay for urinary paracetamol metabolites and its use to characterize the C3H mouse as a model for paracetamol metabolism studies.

A high performance liquid chromatographic (HPLC) assay for unchanged paracetamol and its glucuronide, sulphate, cysteine and mercapturic acid conjugates in the urine of man, mouse and rat is described. The method is simple, rapid and reproducible. The metabolite assay has been used to characterize the male C3H mouse, which shows sensitivity to paracetamol toxicity similar to man, as a model for paracetamol metabolism studies. In male C3H mice there was no evidence to suggest saturability of the glucuronidation pathway on increasing the paracetamol dose from 50 to 300 mg/kg. By contrast, the metabolic ratio and fractional excretion of both the sulphate and glutathione-derived conjugates decreased with increasing paracetamol dose. For animals administered a 200 mg/kg dose of paracetamol, pretreatment with phenobarbitone or 3-methylcholanthrene increased the fractional excretion and metabolic ratio of the glutathione-derived and glucuronic acid conjugates. Piperonyl butoxide pretreatment of animals administered the same dose of paracetamol inhibited glutathione and glucuronic acid conjugation.

Acetaminophen↗