Of 560 consecutive, newly diagnosed untreated patients with B CLL submitted for chromosome study, G-banded karyotypes could be obtained in 480 cases (86%). Of these, 345 (72%) had normal karyotypes and 135 (28%) had clonal chromosome abnormalities: trisomy 12 (+12) was found in 40 cases, 20 as +12 alone (+12single), 20 as +12 with additional abnormalities (+12complex). Other frequent findings included abnormalities of 14q, chromosome 17, 13q and 6q. The immunophenotype was typical for CLL in 358 patients (CD5+, Slg(weak), mainly FMC7-) and atypical for CLL in 122 patients (25%) (CD5-, or Slg(strong) or FMC7+). Chromosome abnormalities were found significantly more often in patients with atypical (48%) than in patients with typical CLL phenotype (22%) (P < 0.00005). Also +12complex, 14q+, del6q, and abnormalities of chromosome 17 were significantly more frequent in patients with atypical CLL phenotype, whereas +12single was found equally often in patients with typical and atypical CLL phenotype. The cytomorphology of most of the +12 patients was that of classical CLL irrespective of phenotype. In univariate survival analysis the following cytogenetic findings were significantly correlated to a poor prognosis: chromosome 17 abnormalities, 14q+, an abnormal karyotype, +12complex, more than one cytogenetic event, and the relative number of abnormal mitoses. In multivariate survival analysis chromosome 17 abnormalities were the only cytogenetic findings with independent prognostic value irrespective of immunophenotype. We conclude that in patients with typical CLL immunophenotype, chromosome abnormalities are somewhat less frequent at the time of diagnosis than hitherto believed. +12single is compatible with classical CLL, and has no prognostic influence whereas chromosome 17 abnormalities signify a poor prognosis. In patients with an atypical CLL immunophenotype, chromosome abnormalities including +12complex, 14q+, del 6q and chromosome 17 are found in about 50% of the patients, and in particular chromosome 17 abnormalities suggest a poor prognosis.

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Increased lymphocyte ATP-ase activity in patients with carcinomas of the oral cavity and larynx.

Increased mitochondrial ATP-ase activity was found in circulating lymphocytes from 5 out of 8 patients with carcinoma of the oral cavity or oropharynx and from 5 out of 10 patients with carcinoma of the larynx. In a majority of these cases changes in the ATP-ase activity after treatment paralled to the clinical result of treatment. It is suggested that determination of ATP-ase activity of circulating lymphocytes is of diagnostic value in patients with oral, oropharyngeal and laryngeal carcinomas as well as prognostic value after treatment of the patients.

Adenosine Triphosphatases↗

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Lymphocyte ATPase activity in patients with carcinoma of the larynx. A follow-up study on 45 patients.

Mitochondria-related Mg ATPase activity was assayed in lymphocytes isolated from the blood in 45 patients with epidermoid carcinomas of the larynx. Increased activity was found in 11 patients and normal or subnormal activity in the remaining cases. No correlation could be demonstrated between the magnitude or the spreading of the malignancy as recorded by the TNM classification and the ATPase activity. In 36 patients determinations were carried out repeatedly during a period from 6 to 20 months following therapy and after 3 years a final follow-up on all patients was done. All patients received high-voltage radiotherapy and in 7 patients additional partial or total laryngectomia was carried out, one patient had bilateral neck dissection. After radiotherapy the ATPase activity decreased significantly in 17 patients while it either remained normal or increased temporarily in the rest. No correlation was found between the course of the disease and the initial ATPase activity or the pattern of variation in ATPase activity following therapy. Contrary to what resulted from a preliminary study, it is concluded that determination of lymphocyte ATPase activity is of no diagnostic value in patients with laryngeal carcinoma, nor does it offer any prognostic help in determining which patients may relapse or develop metastatic disease.

Adenosine Triphosphatases↗