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Biomedical subjects

J E Haky

Publications and source records attributed to J E Haky.

2 recordsLinked to original sources

Automated gas chromatographic method for the determination of residual solvents in bulk pharmaceuticals.

A generalized gas chromatographic method is described for the determination of a wide variety of residual solvents and other volatile impurities in bulk pharmaceuticals. The method employs a highly selective graphitized carbon-black stationary phase, which is demonstrated to retain compounds on the basis of a linear combination of their boiling points and molecular volumes (i.e., molecular weight divided by density). An autosampler is utilized to optimize injection precision and to provide for high sample throughput. Analytical data from replicate determinations of seven representative compounds are reported, and it is shown that calibration of the chromatographic systems against external standards produces comparable results to those obtained by standard addition techniques.

Autoanalysis

Automated data processing for chromatographic assay method validations.

A set of computer programs has been developed for the automated transfer, storage, and processing of data related to chromatographic pharmaceutical assay method validations. These programs calculate and report statistical parameters relating to the resolution, linearity of response, and precision of an assay method based upon peak width, retention time, and integration data provided by a commercial chromatographic data system. Utilization of an interface between the data system and a microcomputer minimizes manual handling of all data. Techniques and equations used in the development of the software are described, and an application to the validation of a high performance liquid chromatographic assay method for a bulk drug substance is demonstrated.

Chromatography, High Pressure Liquid