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Biomedical subjects

J E Berk

Publications and source records attributed to J E Berk.

At least 55 records · Page 3Linked to original sources
PubMed1975

Does human pancreas contain salivary-type isoamylase?

Amylase isoenzyme analysis was made of extracts of normal human pancreatic tissue by first conducting ion exchange chromatography of the purified material. This gave evidence of only pancreatic type (P-type) isoamylase for all purposes. However, when effluent fractions in which salivary type isoamylase would ordinarily be expected to be present were harvested, pooled, concentrated, and rechromatographed, the pancreatic extracts were found to contain some salivary type (S-type) isoamylase. The latter accounted for approximately 0-8 to 1-7% of the total recovered amylase activity. This finding of S-type isoamylase in normal human pancreas potentially has important bearing on the interpretation of isamylase analysis.

Adult↗
PubMed1975

Constipation of prolonged duration.

A case is reported of a 19-year old man who went periods of four and a half and four months without a bowel movement and who experienced no distress throughout these periods except for late appearing abdominal cramps.

Adult↗
PubMed1975

Clinical application of amylase isoenzyme analysis.

Amylase isoenzyme analysis of serum, urine and other bodily fluids using dextran gel and ion exchange chromatography provides a new dimension in the identification of pancreatic disease. Examples are described that illustrate the clinical application of such analysis and the usefulness of the information that may be derived.

Adult↗
PubMed1974

The nature and significance of hyperamylasemia follwing operation.

Total serum amylase activity was found to be significantly elevated postoperatively in 11 (10%) of 110 patients undergoing various surgical procedures. Isoamylase analysis revealed that the rise was chiefly in the pancreatic-type isoamylase in seven of the 11 patients showing postoperative serum amylase elevations; in the other four patients, the elevation occurred principally in the salivary-type isoamylase. These data demonstrate that postoperative hyperamylasemia occurs surprisingly often and that serum amylase activity may rise even when the surgical procedure is extra-abdominal. Moreover, elevation of serum amylase activity after surgery is not necessarily an indication of pancreatitis and may reflect instead a rise in salivary-type isoamylase.

Abdomen↗
PubMed1973

Simulation of macroamylasaemia by salivary-type ('S type') hyperamylasaemia.

Hyperamylasaemia with low or normal urinary amylase excretion in the face of normal renal function has been noted to be indirect evidence for the existence of macroamylasaemia. A more refined indicator is a low ratio of renal amylase clearance to creatinine clearance. This report describes observations made in three patients, each of whom displayed the indirect features suggestive of macroamylasaemia. In none of these patients, however, could a serum macroamylase be demonstrated by chromatography. Ultracentrifugation was also done in one of the patients and likewise failed to disclose a macroamylase in the serum. When the isozyme nature of the serum amylase in each of these patients was examined, there was found to be a marked rise in activity of the salivary-type isoamylase (;S-type amylase'). The pancreatic-type isoamylase (;P-type amylase') was normal in one patient and perhaps slightly above normal in the other two. These data indicate that (1) S-type hyperamylasaemia may exhibit the indirect criteria taken to indicate macroamylasaemia and thereby simulate the latter disorder; and (2) unequivocal identification of macroamylasaemia requires the direct demonstration of the presence in the serum of a macromolecular amylase complex.

Amylases↗