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Biomedical subjects

J E Bennett

Publications and source records attributed to J E Bennett.

At least 181 records · Page 10Linked to original sources
PubMed1973

Stability of amphotericin B in infusion bottles.

Intravenous solutions of amphotericin B in 5% dextrose water with or without hydrocortisone or heparin demonstrated no appreciable loss of activity when exposed to fluorescent light for up to 24 h at 25 C (room temperature).

Amphotericin B↗
PubMed1973

Growth of Cryptococcus neoformans within human macrophages in vitro.

Macrophages cultured from human peripheral blood monocytes were infected with Cryptococcus neoformans in vitro. Although C. neoformans were actively ingested, there was no detectable intracellular killing by macrophages. Over 2 days or more, intracellular fungi grew more rapidly than a corresponding inoculum of extracellular cryptococci growing in tissue culture medium containing human serum. Macrophages were induced to develop into cells which appeared to be activated by morphological and phagocytic criteria. However, these activated cells did not acquire an ability to kill or inhibit intracellular growth of C. neoformans. There were no detectable differences between macrophages from normal subjects and those from cryptococcosis patients.

Cells, Cultured↗
PubMed1972

Pharmacological studies with 5-fluorocytosine.

A cylinder plate bioassay for 5-fluorocytosine (5-FC) is described which permits determination of 5-FC concentrations in biological fluids in the presence of amphotericin B. Using this assay, we determined serum concentrations in 12 patients after a single dose of drug and in 8 patients receiving daily 5-FC at 6-hr intervals (4 to 120 days). Drug was detectable in serum as early as 0.5 hr and concentrations were measurable as long as 24 hr after a dose, regardless of renal function. Peak concentrations occurred 6 hr after the initial drug dose, but were seen between 1 and 2 hr after a dose in all patients receiving a minimum of 4 days of therapy. Mild to moderate renal impairment produced marked increases in peak 5-FC concentrations in the serum of a group of eight patients on three different dosage schedules. Five patients were studied before and after amphotericin B-induced renal insufficiency. Peak concentrations increased from 14 to 142% concomitant with the change in renal function. Parallel studies in rabbits confirmed the results in our patients. 5-FC half-life in the serum of 10 rabbits increased from 3.35 +/- 0.27 to 24.63 +/- 0.70 hr after experimentally induced acute renal failure. Concentrations of 5-FC in the cerebrospinal fluid of five patients ranged from 17 to 62 mug/ml and were 74.4 +/- 5.6% of simultaneously determined serum concentrations. The effect of renal function on 5-FC concentrations in cerebrospinal fluid was similar to that seen with serum.

Adult↗