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Biomedical subjects

J Durand

Publications and source records attributed to J Durand.

At least 199 records · Page 11Linked to original sources
PubMedDate not supplied

Inositol phosphate turnover in human airways: effect of arachidonic acid metabolism.

The aim of this study was to examine the role of the phospholipase A2-arachidonic acid (PLA2-AA) pathway in response to histamine and the possible effect of AA metabolites on the generation of inositol-phosphates (IPs) in airway smooth muscle cells (SMC). Radiolabelled IPs-were analyzed by high-performance liquid chromatography (HPLC). Indomethacin, a cyclooxygenase inhibitor, potentiated the IP production evoked by a 5-sec application of histamine, while it decreased the effect of a 5-min incubation with the agonist, suggesting a time-dependent modulation of phospholipase C (PLC) activity by prostaglandins (PGs). Exogenous AA elicited the generation of IPs; this effect was suppressed by cyclooxygenase inhibition. Therefore, several steps in the AA metabolic pathways appear to modulate the production of IPs in human airway SMC.

Arachidonic Acid↗
PubMedDate not supplied

Immortalized goat milk epithelial cell lines replicate CAEV at high level.

Primary milk epithelial cells were isolated from CAEV-uninfected goats and three cell lines designated TIGMEC-1, TIGMEC-2 and TIGMEC-3 were established. The three cell lines retained their morphological characteristics of epithelial cells and expressed specific epithelial cytokeratin marker as well as the immortalizing SV40 large T antigen. The kinetics of growth of TIGMEC1, TIGMEC2 and TIGMEC3 cell lines showed a doubling time of 24-48 hours while the parental cell lines became senescent after the passage 6 in cell culture. Like the parental primary cells, the three cell lines were found to be highly sensitive to CAEV-pBSCA, an infectious molecular clone of CAEV-CO strain, and to a French isolate CAEV-3112. TIGMEC cell lines infected with CAEV-pBSCA became chronically infected producing high virus titers in absence of cytopathic effects. These cell lines may be useful for study of the possible physiological alterations in mammary epithelial cells infected with small ruminant lentiviruses and their consequences on milk quality. On an other hand, these cell lines can be used to study their role in virus transmission and pathogenesis.

Animals↗