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Biomedical subjects

J Duncan

Publications and source records attributed to J Duncan.

At least 163 records · Page 9Linked to original sources
PubMed1976

A unique course in anthropology.

The course in forensic anthropology presented at Florida State University is designed to train criminal investigators in the application of physical anthropological and archaeological techniques to the investigation of buried bodies. Modification of archaeological field techniques by experimentation using artificial graves allows the recovery of fragile and easy to overlook evidence and ensures the recovery of all possible material for analysis by the physical anthropologist. An introduction to physical anthropology enables the criminal investigator to effectively communicate his needs and understand the type of information required for adequate identification of remains by a physical anthropologist.

Anthropology, Physical↗
PubMed1976

Enzymatic degradation of uracil-containing DNA. II. Evidence for N-glycosidase and nuclease activities in unfractionated extracts of Bacillus subtilis.

Further studies have confirmed our earlier observations that in the presence of EDTA, degradation of phage PBS2 [3H]uracil-labeled DNA is effected by an N-glycosidase activity in extracts of Bacillus subtilis that removes free uracil from DNA. In addition, such extracts contain a nuclease activity that attacks PBS2 DNA in the presence of CaCl2. The nuclease activity is not observed under conditions that inactivate N-glycosidase activity but does attack DNA that has been preincubated to remove uracil by N-glycosidase action. We therefore postulate that the nuclease requires N-glycosidase action to generate substrate for its activity, i.e., the nuclease appears to attack depyrimidinated sites rather than uracil sites in phage PBS2 DNA.

Bacillus subtilis↗
PubMed1973

Assimilation of hydrocarbons by Pseudomonas strains isolated from human clinical specimens.

Twenty strains of Pseudomonas isolated from human clinical specimens on routine laboratory media, without hydrocarbon enrichment and unselected for their growth on hydrocarbons, were tested for their ability to utilize a series of eight n-alkanes and two 1-alkenes as a sole carbon and energy source for growth. Hydrocarbon assimilation does occur with such isolates relative to the chain length and the degree of saturation of the hydrocarbon. The data presented show that all 16 stains of Pseudomonas aeruginosa studied grew readily on dodecane through hexadecane and on 1-hexadecene. In addition, most strains of this species grew on undecane and 1-dodecene after prolonged incubation. There was a long lag period, usually a minimum of 4 days, before onset of growth on any hydrocarbon. In no case did hexane or decane support growth. Two strains each of P. maltophilia and P. stutzeri were unable to grow on any of the hydrocarbons tested. Hexane in concentrations above 1% (vol/vol) is bactericidal toward the Pseudomonas inoculum. It is toxic even to cells utilizing different hydrocarbon for growth. The addition of 1% hexane to 1% (vol/vol) hexadecane markedly prolonged the lag phase of P. aeruginosa utilizing the hexadecane for growth.

Alkanes↗
PubMed1967

Molluscicidal properties of organotin and organolead compounds.

Triphenyltin acetate has proved successful as a molluscicide in the laboratory and in small-scale field trials. However, in view of its cost and its toxicity to swamp rice at molluscicidal concentrations, an effort has been made to explore the molluscicidal value of other organotin and organolead compounds. Those that proved most toxic to snails were screened in the laboratory against swamp rice in order to obtain some measure of their phytotoxicity. This study yielded 6 compounds that were relatively non-toxic to rice. One of these, triphenyllead acetate, has been formulated as an emusifiable concentrate for use in the field and the results of trials in two parts of Tanzania are reported. Chemical analyses of water treated with triphenyllead acetate are discussed, and the reasons for the disappearance of the compound in the field are investigated. Some preliminary information on the toxicity of triphenyllead and triphenyltin derivatives to other organisms is also given.

Animals↗