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Biomedical subjects

J Duheille

Publications and source records attributed to J Duheille.

At least 37 records · Page 2Linked to original sources
PubMed1984

Mucosal immunity.

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Glomerulonephritis, IGA↗
PubMed1983

Immunoglobulin A nephropathy. Quantitative immunohistomorphometry of the tonsillar plasma cells evidences an inversion of the immunoglobulin A versus immunoglobulin G secreting cell balance.

Primary IgA nephropathy (Berger's disease) is characterized by renal deposits of IgA, the origin of which is still unknown. However, several clinical and biological findings suggest that these immunoglobulins might have a mucosal origin, and that such patients should present mucosal abnormalities. This paper reports the results of the immunohistomorphometrical analysis of tonsillar plasma cells from seven patients suffering from Berger's disease and seven controls also with recurrent tonsillitis. IgG, IgA, and IgM-secreting cells were enumerated after immunofluorescent staining of serial frozen-cut sections from 20 tonsils. In controls, a predominance of the IgG-secreting population, similar to this reported in the literature was observed (65% of IgG secreting cells and 29% of IgA plasma cells), while an inversion in the patients' plasma cells percentages was evidenced (IgG:37%, IgA:56%). This increment in the IgA population was paralleled by an augmentation of the number of dimeric IgA-secreting cells (75% of IgA plasma cells), stained both for cytoplasmic IgA and J chain. In controls, the latter cells were in similar proportions as previously reported by others (45% of IgA plasma cells). These results demonstrate an imbalance in the IgA-producing system of patients with Berger's disease, which is in keeping with the hypothesis favoring a mucosal origin for the mesangial IgA present in their kidneys.

Adolescent↗
PubMed1983

[Antigenic markers of immunocompetent cells in rheumatoid synovial membrane].

Immunologic characteristics of cells present in rheumatoid synovium from 15 patients were analyzed either "in situ" on frozen sections or after elution. Monoclonal antibodies directed against T cell subpopulation antigens and anti-Ia-like were used. T cells bearing the phenotype T3+, T4+, Ia+ are numerous, and they appeared gathered in lymphoid follicles and in perivascular areas. They are in close relationship with large Ia+ cells probably macrophagic in nature. Numerous dendritic cells as well as intimal cells are also Ia+. Some ratios T8+/T4+ cells are higher than results previously reported. This discrepancy could be related to drugs received by the patients before surgery.

Anti-Inflammatory Agents↗
PubMed1983

Graves' disease: in situ localization of lymphoid T cell subpopulations.

An in situ analysis of immunological features in thyroids from 15 Graves' disease patients has been performed. This study included a search for immune complexes visualized near the follicle's basement membranes with fluorescent rabbit antisera to human IgG, IgA, IgM, C1q, C3 and C9. Cellular immunity was investigated on the humoral side by visualization of B cells and plasma cells. Two series of monoclonal antibodies (OKT3, 4 and 8, Leu 1, 2a and 3a) were used to label the infiltrating cells' membrane. These studies demonstrated the prevalence of T cells, a majority of them with OKT8/Leu 2a suppressor/cytotoxic phenotype. No correlation was found between this observation and peripheral blood T cell subsets analysis.

Antibodies, Monoclonal↗
PubMed1982

Improvement of cellular immunity and IgA production in immunodeficient children after treatment with synthetic serum thymic factor (FTS).

Three children with IgA and IgE deficiency and T-cell defects (two related patients with ataxia telangiectasia and one with common variable immune deficiency) were treated with synthetic serum thymic factor (FTS) intravenously. A reduction in frequency and severity of infection was noted concomitantly with improvement in cell-mediated-immunity tests. Serum IgA, which was absent in two patients, appeared within 4 weeks of treatment and increased significantly in the third patient. Specific antibodies against vaccination antigens appeared for the first time or increased to titres higher than ever before. In two patients, transient interruption of FTS administration was followed by a regression of the immunological improvement, but this disappeared after the treatment was started again.

Adolescent↗
PubMed1982

Familial hyperthyroidism without evidence of autoimmunity.

Thirty-four members of a single family were studied and 9 of them were found to be suffering from hyperthyroidism associated with diffuse goitre. Exophthalmos was absent and transmission seemed to be independent of HLA type. Four of the 9 were studied prior to treatment but in all cases serum immunoglobulin levels were normal, antithyroglobulin and antimicrosomal antibodies absent, thyroid stimulating antibodies negative and the lymphocyte transformation responses to mitogens not different from those of controls. The results of testing the euthyroid family members were similarly negative, except in the case of a woman with type I diabetes mellitus who showed a low titre of antimicrosomal antibody. Seven of the patients underwent subtotal thyroidectomy. Lymphocytic infiltration of the excised portion was rarely present. Four of the glands were subjected to immunofluorescent and electron microscopy but neither immunosecreting cells nor immune complex deposits were found. These results point to the existence of a non-autoimmune form of goitrous hyperthyroidism, different from Graves' disease.

Adolescent↗
PubMed1982

IgA nephropathy: characterization of the polymeric nature of mesangial deposits by in vitro binding of free secretory component.

IgA nephropathy, as Berger defined it, is characterized by mesangial deposits of IgA, which are easily visualized by immunofluorescence on kidney biopsies. The structure (mono- or dimeric) of these IgA has not been clearly defined so far. Fifteen renal biopsies were studied to find out whether these IgA are serum monomers, or are polymers from a different origin. This was done by tissue fixation in vitro of free secretory component, which was then visualized by immunofluorescence (IF). In all 15 cases, the IgA deposits were shown to lack bound secretory component, but were able to bind, specifically, with the free secretory component. The presence of J chain in these deposits was also evidenced by indirect IF. These findings favour the hypothesis that these immunoglobulins are polymeric.

Fluorescent Antibody Technique↗
PubMed1982

In vitro induction of monoclonal antibody-defined T-cell markers in lymphocytes from immunodeficient children by synthetic serum thymic factor (FTS).

Lymphocytes from five children suffering from ataxia telangectasia or various unclassified immune deficiencies were tested in vitro for their sensitivity to synthetic serum thymic factor (FTS). The percentages of cells bearing T cell markers were elevated after incubation with FTS at graded concentrations (0.25, 2.5 and 25 ng/ml), by microlymphocytotoxicity or indirect immunofluorescence, using monoclonal anti-Lyt1 antibodies. In four cases, more than 30% of the non-T non-B cells acquired the Lyt1 T cell marker. These four children had low levels of circulating FTS. In the fifth child, who had a normal serum FTS level, and in two age-matched controls, there was no significant increase in the percentage of cells bearing the T marker.

Adolescent↗
PubMed1980

Scanning electron microscopic study of microcrystals implicated in human rheumatic diseases.

Scanning electron microscopy has been used in conjunction with wavelength dispersive X-Ray spectroscopy and in correlation with X-Ray diffraction to define the populations of crystals present in rheumatic diseases. Microcrystals of monosodium urate, triclinic and monoclinic calcium pyrophosphate dihydrate, apatite, calcium hydrogen phosphate dihydrate and corticosteroids, among others, have been found in synovial fluid, in the intraarticular tissues (fibrocartilage, cartilage, and synovial membrane), and in the periarticular tissues (tendons and ectopic calcifications). Scanning electron microscopy in conjunction with wavelength dispersive X-Ray spectroscopy has made it possible to detect microcrystals, even isolated, to describe their morphologies, and to study their relations with the cells of the synovial fluid and with the collagenous and cellular structures of the synovial membrane and of the cartilage. It cannot replace X-Ray diffraction for the conclusive identification of microcrystals, but it can certainly help to improve the analysis of the various populations of crystals present in articular and periarticular rheumatic diseases.

Arthritis, Rheumatoid↗
PubMed1979

[Study of cellular elements in synovial fluid in scanning electron microscopy].

Scanning electron microscopy allows a tridimensional morphological study of the cellular elements of the pathological human synovial fluid. The cells are grouped into two populations: macrophage cells and round cells, whose differentiation is more difficult: polynuclears, lymphocytes and synovial cells. The combination of scanning electron microscopy and X-ray diffraction makes it possible to describe and indentify crystals of sodium urate and of triclinical, dehydrated calcium pyrophosphate.

Arthritis, Rheumatoid↗
PubMed1977

[Scanning electron microscopy study of the synovial membrane in rheumatoid arthritis].

A comparative study under the scanning electron microscope of rheumatoid synovial membranes, 5 arthrosic synovial membranes, one tuberculous membrane, and 3 normal synovial membranes showed the pathological changes in the synovial membrane due to rheumatoid arthritis: Inflammatory aspect of the synovial fringes, surrounded by turgid and proliferative villous processes. Granular appearance of the endo-articular surface like a "pebble beach". Dome-shaped synoviocyte layer, standing out well above the subjacent intimal layer, associated with small round cells, also with a raised margin, covering most of the endo-articular surface. Plasma membrane of the superficial synovial cells, covered with numerous and various differentiations and abundant microvillous processes. Reticular deposits or layers, covering the apical poles of the synoviocytes.

Arthritis, Rheumatoid↗
PubMed1977

[Identification of the crystals observed in the destructive arthropathies of chondrocalcinosis].

Study of the synovial membrane and cartilage demonstrating two destructive arthropathies of the knee diagnosed in subjects with articular pseudogout. Scanning electron microscopy reveals the presence of many crystals on the surface of the cartilage and the synovium and in the depth of the cartilage. These can be grouped into two families on the basis of dimensions and morphology. The first consists of those shaped like arrowheads and are large (80-100 microns long.) They were formally identified using Weissenberg's technique; dihydrated calcium hydrogenophosphate (CaHPO4.2H2O) is involved here. In the second family the crystallogenesis is different (prism or lozange-shaped) and the crystals themselves have not been identified with certainty because of their tiny size (20 microns). The hypothesis is proposed that the crystals of dihydrated calcium hydrogenophosphate properly belong to the destructive arthropathies of pseudogout; while not necessarily its cause, they may help explain its development.

Aged↗
PubMed1976

[Autoimmune mechanisms and post-anesthetic hepatitis].

After a brief review of clinical, biological and immunological features of post anesthesia hepatitis and hepatic auto-immunity, the authors attempt to determine whether certain forms of post-anesthesia hepatitis can be placed under the heading of autoimmune liver disorders, the mechanisms by which auto-immunity can be induced and modulated and the practical implications of these concepts, namely with regard to detection of subjects at risk.

Anesthetics↗