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Biomedical subjects

J Du

Publications and source records attributed to J Du.

At least 253 records · Page 14Linked to original sources
PubMed1993

[Preliminary study on monoclonal antibodies against Cysticercus cellulosae].

In order to produce valuable monoclonal antibodies for immunodiagnosis of cysticercosis, hybridoma technique was adopted using the fusion of splenic lymphocytes from BALB/c mice immunized with Cysticercus cellulosae antigens and the myeloma cell line NS-1. Through screening and cloning, three monoclonal antibody cell lines have been established. Immunoglobulin subclasses of 3 lines were determined to be IgG1. These monoclonal antibodies were found to be highly specific and without cross reaction with Echinococcus granulosus hydatid cyst and Cysticercus fasciolasis. Antigen fractions specifically reacting to monoclonal antibodies were demonstrated with Western blot method. The results showed that specific reaction bands of SCW located in 25 kDa and 17 kDa regions.

Animals↗
PubMed1993

Retinal pigment epithelial transplants and retinal function in RCS rats.

PURPOSE: To determine if retinal pigment epithelium (RPE) transplantation maintains visual function in Royal College of Surgeons (RCS) strain of rats. METHODS: Twelve RCS rats received RPE transplants at 16 to 20 days after birth. The retinas were studied electrophysiologically and histologically from 3 to 10 months after transplantation and compared with 11 RCS controls and 11 normal rats of comparable ages. A microelectrode was guided to the transplant site visible by its pigmentation in the albinotic RCS retina to detect responses. RESULTS: Spontaneous ganglion cell activity was present in all retinas. Ganglion cell responses to light were detected in 9 of the 12 transplant eyes but not in any of the 11 controls. 96, 44, 140 units were encountered and 30%, 0%, 97% were driven by light respectively in transplant, control, and normal retinas. In transplants 36%, 29%, and 28% were driven at 3 to 4, 6 to 7, and 10 months after transplantation, respectively. Intraretinal ERGs with both a- and b-waves were recorded in 5 of the 8 transplants studied. None of the RCS controls studied had an IERG. The average IERG was 2.5 microV (SD = 1.9) in transplants and 59 microV (SD = 19) in normal retinas. The electrode track was traced to the transplant site in six of the seven retinas that were responsive to light and examined histologically. CONCLUSION: RPE transplants to RCS rats maintain retinal function in the transplant site for long periods of time.

Animals↗
PubMed1992

Monitoring photoreceptor transplants with nuclear and cytoplasmic markers.

Two methods are described for identifying transplanted photoreceptors in a foreign host retina. One involves the use of [3H]thymidine to label the nuclei of photoreceptors which are dividing for 1 week after birth in myomorphic retina. These photoreceptors can be identified by autoradiography. The second involves the use of a transgenic mouse carrying a bovine rhodopsin promoter in tandem with the bacterial LacZ gene. These mice express beta-galactosidase in their rods. X-gal reaction allows these rods to be identified by routine light and electron microscopy. These methods have been used to follow photoreceptor transplants in adult Royal College of Surgeons strain rat and C3H mouse mutants which have lost virtually all their photoreceptors. Dissociated photoreceptors transplanted to the subretinal space of these animals survive for at least 3 months. The inner segment, cell body, and synaptic terminal of these transplanted photoreceptors remain morphologically normal; the outer segment, however, becomes rudimentary.

Animals↗
PubMed1992

Alterations of spinal dorsal horn substance P following electroacupuncture analgesia--a study of the formalin test with immunohistochemistry and densitometry.

Substance P (SP), released from thin afferent terminals, is believed to be a neurotransmitter for pain transmission in the spinal dorsal horn. It has been demonstrated that in addition to analgesia, morphine increases the accumulation of SP possibly due to the inhibition of its release. The present work investigated the level of spinal SP like immunoreactivity (SPLI) following electroacupuncture analgesia in rats using immunohistochemistry and image analysis. Experiment results revealed that formalin injected into the hind paw elicited marked pain response and accumulation of SP in the spinal dorsal horn. Electroacupuncture of Tsu-San-Li could depress the pain response, however increasing further the SP accumulation. It is thus suggested that pain stimulation itself may activate the endogenous opioid mechanism to inhibit SP release and acupuncture is able to enhance the process. This may be one mechanism of acupuncture analgesia.

Animals↗
PubMed1992

[Effect of Chinese-made vesnarinone on experimental heart failure of dog].

Intravenous drip of sodium pentobarbital 6 mg/kg per min induced obvious heart failure of anesthetic dogs (n = 11) followed by an infusion of 0.25 mg/kg per min to maintain the heart failure state. The Chinese-made vesnarinone 3 mg/kg was injected, followed by an infusion of 0.1 mg/kg per min, or the solvent in the same volume, for 30 min. Vesnarinone increased significantly and instantly the cardiac output and left ventricular maximum + dp/dt, which almost recovered to normal at the end of the infusion and also significantly increased 30 min after administration. The positive inotropic effect of vesnarinone was not accompanied by an increase in the heart rate and the blood pressure. The results of our experiment reveal that the Chinese-made vesnarinone has a potent and relatively selective positive action on heart failure of dogs.

Animals↗
PubMed1992

Reconstruction of degenerate rd mouse retina by transplantation of transgenic photoreceptors.

Photoreceptors from neonatal transgenic mice with normally developing retinas were transplanted to the subretinal spaces of 2-3-month-old rd mutant mice that lack photoreceptors. The transgenic mouse photoreceptors express high levels of the lac Z reporter gene product, beta-galactosidase, which facilitated tracking the transplanted cells. Two sources were used for these cells: (1) dissection of retinal microaggregates containing photoreceptors and (2) papain-dissociated photoreceptors. Host retinas were examined after transplantation. Both methods led to survival of photoreceptors for at least 2 mo after transplantation. Relatively mature outer segments were found only in transplanted microaggregates; this occurred optimally when the cells were adjacent to the retinal pigment epithelium (RPE). beta-galactosidase-labeled outer segments associated closely with the apical processes of the host RPE, which, together with labeled phagosomes in the RPE cells, suggested functional interaction between the transplanted photoreceptors and the host RPE. This study is the first to the authors' knowledge to show electron microscopically that a morphologically normal-appearing photoreceptor layer can be reconstructed in an otherwise photoreceptorless retina.

Animals↗
PubMed1991

Radon exposure, cigarette smoking, and other mining experience in the beaverlodge uranium miners cohort.

A nested case-control study within the Beaverlodge Uranium Miners Cohort was undertaken to assess any possible contribution of confounding by smoking and other mining experience to the risk estimate derived from the original cohort study. Next of kin have been interviewed for 46 lung cancer cases and 95 controls enrolled in the Beaverlodge Uranium Miners Cohort Study who died between 1950 and 1980. Confounding by cigarette smoking and other mining experience appears unlikely to have contributed to the relative risk coefficient for exposure to Rn decay products derived in the parent study. Data for smoking and exposure to Rn decay products are consistent with a multiplicative model, although considerable caution must be applied to this interpretation.

Case-Control Studies↗
PubMed1991

Reversed polarity of Na(+) -K(+) -ATPase: mislocation to apical plasma membranes in polycystic kidney disease epithelia.

Autosomal dominant polycystic kidney disease (ADPKD) is a genetic disorder in which renal tubules become enormously enlarged due to fluid accumulation. Na(+) -K(+) -ATPase was compared in normal and cystic regions of whole kidneys and in confluent primary cultures of microdissected renal tubule and cyst-lining epithelia. Immunostaining with antibodies directed against the Na(+) -K(+) -ATPase catalytic alpha-subunit was confined to apical, luminal plasma membranes of ADPKD epithelia, which was a complete reversal of the normal renal tubule polarized location in basolateral membranes. Mislocated Na(+) -K(+) -ATPase was shown to be functionally active, because identical intense apical staining was observed by use of a cytochemical assay. In addition, biochemical assays showed a significant increase in these ouabain-inhibitable Na(+) -K(+) -ATPase specific activity levels in ADPKD kidneys compared with age-matched normal kidneys. Specific binding of [3H] ouabain was not only increased but also confined to the apical membrane vesicles prepared from cystic regions of ADPKD kidneys compared with normal age-matched controls, in which binding was confined to basolateral membrane vesicles. Although steady-state levels of Na(+) -K(+) -ATPase alpha- and beta-subunit in mRNAs were increased somewhat in ADPKD kidneys, this alone was not sufficient to account for the observed activation. Confluent ADPKD epithelia grown on dual-chamber, permeable membrane supports also showed reversed polarity of 22NaCl vectorial transport, because this was from basal to apical media compartments. Because this transport could also be blocked by ouabain, this suggested apical Na(+) -K(+) -ATPase was responsible and implicated altered polarity of Na(+) -K(+) -ATPase and resultant Na+ secretion as a mechanism for cyst formation in ADPKD. Because no reversal of polarity of other basolateral or apical membrane proteins was detected, an intracellular sorting defect specific for Na(+) -K(+) -ATPase is proposed.

Biological Transport↗
PubMed1991

Survival and synapse formation of transplanted rat rods.

Isolated rods enzymatically removed from normal adult rat retina have been transplanted to the subretinal space of adult rats with a retinal dystrophy which has destroyed almost all the photoreceptors. These transplanted rods survive for months after transplantation during which time they form synapses with other retinal cells. Rod spherules with large amounts of synaptic vesicles and synaptic ribbons are found forming discreet contacts with pre- and post-synaptic densities in arrangements closely resembling those seen in the normal retina.

Animals↗
PubMed1991

Transplanted photoreceptors identified in dystrophic mouse retina by a transgenic reporter gene.

Dissociated photoreceptor cells from a transgenic strain of mice, containing a bovine promoter lac Z gene construct, were transplanted to the dystrophic retinas of a strain of mice (C3H, rd/rd) without obvious photoreceptors. The transgenic photoreceptor cells expressed beta-galactosidase and were distinguishable from the host retinal cells by light and electron microscopy after the 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside histochemical reaction. These results showed that transplanted transgenic photoreceptor cells survived at least 1 month in the host retina and had a primitive outer segment and a well-developed synaptic terminal.

Animals↗
PubMed1991

[Hypocrellin A induced photodamage to the fluidity of human erythrocyte membranes and membranes of some phospholipid liposomes].

In this paper, the photodamage of Hypocrellin A to the fluidity of human erythrocyte membranes and some kinds of membranes of phospholipid liposomes was investigated by measuring the changes in fluorescence polarization of the membranes. The results showed that the photosensitization effect of HA caused the decrease of membrane fluidity of the phospholipid (DPPC, DPPC/DPPE, phospholipid of erythrocyte membranes) liposomes. The DPPC and DPPC/DPPE liposomes were more sensitive to the damage than the phospholipid liposomes of erythrocyte membranes. To human erythrocyte membranes, the photodamage effect of HA caused its fluidity first increased and then, with the increment of illumination time, decreased. To spectrin-depleted and trypsin-treated erythrocyte membranes, this kind of change in fluidity was inhibited. All of the results indicated that phospholipids and proteins play different roles in the photodamage of HA to the fluidity of membranes. Membrane proteins, especially spectrin, were the key factor involved in the changes of the fluidity.

1,2-Dipalmitoylphosphatidylcholine↗
PubMed1991

[Preliminary studies on subtypes of respiratory syncytial virus in China].

An analysis of subtypes of 9 respiratory syncytial (RS) viruses isolated from Guangzhou and Nanjing areas of china was carried out with eight Sweden RS-subtype specific monoclonal antibodies (MAbs) and 7 internal anti-RS MAbs. All these MAbs directed against respectively the large Glycoprotein (G), fusion protein (F), nucleoprotein (NP), and phosphoprotein (P) components of the prototype Long strain of RS virus. The patterns of the reactions of these MAbs to the nine isolated strains of RS virus were compared with indirect immunofluorescence assay (IFA), alkaline phosphoesterase-anti alkaline phosphoesterase (APAAP) enzyme-linked assay and Western blotting. The antigenic variations were founded among the strains of RS virus, and two subtypes allocated to the subtype A and B of RS virus by using the eight RS-subtype specific MAbs. Seven out of the 9 isolated strains of RS virus belonged to the subtype A, and two were being to the subtype B. The antigenic diversities were also founded within the same subtype, and the main pronounced difference were observed on the G glycoprotein by using the internal anti-RS MAbs. These findings are potentially important both for vaccine development and for the understanding of clinical and epidemiological characteristics of RS virus.

Antigenic Variation↗
PubMed1991

[The effect of hypocrellin A on the lipid bilayer of erythrocyte membrane].

In this paper, using human erythrocyte membrane, the effect of Hypocrellin A on the lipid bilayer of the membrane was studied by measuring the change of the fluidity of the membrane, the energy transfer of the fluorescent probes, the shift of the fluorescent emission peaks, and the split of band-a of Hypocrellin A. The results showed that in the presence of HA, the fluidity of erythrocyte membrane was increased, the fluorescence intensity of the probes was decreased, and the fluorescence peaks shifted blue. These phenomena took place more seriously with the increment of HA concentration. Meanwhile, the band-a of HA excitation spectra was splitted. It was suggested from all of the results that HA could significantly perturb the lipid bilayer of erythrocyte membrane, there were interactions existing between the Hypocrellin A and the membrane. The HA was mainly located in the middle range of the membrane lipid bilayer when in high concentration (mainly to the 12-16 positions of the long chain fatty acid).

Energy Transfer↗
PubMed1991

Transplantation of photoreceptors labeled with tritiated thymidine into RCS rats.

Tritiated thymidine was administered to newborn rats to label photoreceptors, about 50% of which are still dividing. These photoreceptors were enzymatically dissociated and separated from the remainder of the retina after the infant rat matured. These labeled photoreceptors were then transplanted into a foreign host retina in the region of the outer nuclear layer. The hosts were ocular, albinotic, Royal College of Surgeons (RCS) rats, congenic to the normal donors and at least 4 months old, a time when virtually all the photoreceptors have degenerated from their retinas. The transplant site was examined at various times after transplantation by light microscope autoradiography. Labeled photoreceptor cell bodies were found in clusters in the outer nuclear layer region for as long as 3 months after transplantation surgery.

Animals↗
PubMed1990

[Mean transit time and partial mean transit time of spirogram in 628 normal adolescents].

The mean transit time(MTT) and partial mean transit time(MTTp) were measured and analysed by "direct method" in 628 cases of normal adolescents in Cheng-du. The results showed that both MTT and MTTp were increased with age and prolonged with the decay of lung volume. Besides, the FVC-t curve was not single exponential in nature. The specific mean transit time (SMTT) was introduced by correcting MTT with 0.5 FVC and represented the MTT when unit volume of gas passed through just at that lung volume. SMTT was highly negatively correlated with age. This reflected the development and growth of the lung recoil and airway conductance in adolescents.

Adolescent↗
PubMed1990

[Transdermal permeability of estradiol through human skin of different body regions in vitro].

Transdermal permeability of estradiol was carried out by using Valia-Chien double-compartment permeation cell for the following 6 skin regions with intact and without stratum corneum: chest, abdomen, hip, upper arm, thigh and back. The estradiol permeation rates and accumulative amounts within 72 h in vitro were examined by HPLC. The results showed that the permeation rates of intact skin from different regions of the body were significantly different (P less than 0.01), and for the skins without stratum corneum over different regions, the permeation rates or the permeation amounts were about 18-55 times higher than that for the intact skin. The results demonstrated that the stratum corneum acts as the rate-limiting barrier in the skin permeation of estradiol, and that the difference in estradiol permeation rates for different skin regions was mainly caused by the different extents of the barrier.

Adult↗