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Biomedical subjects

J Duława

Publications and source records attributed to J Duława.

At least 19 recordsLinked to original sources
PubMed2004

Urinary tract infection--2003.

Urinary tract infections (UTI) are one of the most common and most intensively studied infections encountered in clinical practice. It is, however, an area where significant confusion has existed in agreeing the criteria for diagnosis, the natural history of disease, and the treatment. In the present paper etiology and risk factors for uncomplicated UTI, treatment of UTI, estrogen in UTI treatment in postmenopausal women and UTI in patients with diabetes and renal insufficiency were discussed.

Anti-Bacterial Agents↗
PubMed2001

[Urinary excretion of Tamm-Horsfall protein, albumin and beta-2 microglobulin in children with recurrent urinary tract infection].

In the study 27 children after urinary tract infection (UTI) and 28 healthy children were examined. Vesico-uretic reflux in 10 patients, and in 17 normal urinary excretion were diagnosed. In 11 children the family history was documented. The 17 healthy controls were siblings of children with normal urinary excretion. In all children urinary excretion of albumin, beta 2-microglobulin and Tamm-Horsfall protein (THP) were examined. Children with UTI both with and without urinary obstruction show the increased urinary excretion of THP as compare with healthy children. Healthy siblings of children with UTI show increased urinary concentration of THP as compared with healthy controls. In both examined groups of children with UTI, function of glomeruli, procimal and distal tubuli measured by urinary excretion of albumin, beta 2-microglobulin and THP were normal.

Adolescent↗
PubMed1999

Composition of the sugar moiety of Tamm-Horsfall protein in patients with urinary diseases.

The sugar moiety of Tamm-Horsfall protein (THP) is altered by pathological conditions. The aim of this study was to investigate the composition of THP glycans in urinary diseases. THP was isolated from the urine of patients with urinary tract infection (group A), glomerulonephritis or interstitial nephritis (group B), and Bartter's syndrome (BS) (group C). Monosaccharides, N-glycan profile, THP reactivity with specific lectins and some other proteins were analyzed. THP of patients from groups A, B, and C showed lower amounts of N-acetylgalactosamine (P<0.05, P<0.005, and P<0.05, respectively) than controls; this was reflected in lower reactivity with Phaseolus vulgaris lectin (P<0.005, P<0.05, and P<0.005). Reduced amounts of N-acetylglucosamine were noticed in groups A (P<0. 05) and B (P<0.05). In group A lower amounts of galactose and alpha2, 6-linked sialic acid, as determined by reactivity with Datura stramonium lectin (P<0.005) and Sambucus nigra lectin (P<0.005), were observed. In patients with BS there was a shift from tetrasialylated glycans towards less-sialylated chains. We found also that THP of all patients binds more strongly to IgG(1) (P<0.005, for all patient groups). Our results indicate that the urinary diseases examined affect the THP sugar moiety and the binding of THP to IgG(1).

Acetylglucosamine↗
PubMed1998

Urinary excretion of Tamm-Horsfall protein in normotensive and hypertensive elderly patients.

Tamm-Horsfall protein (THP) is a glycoprotein that is exclusively produced by the kidney in the thick ascending limb of Henle's loop (TAHL). Disturbances of TAHL function are associated with decreased urinary THP excretion. It is well known that renal function declines with advancing age. Moreover, it is suggested that THP may play a role in the pathophysiology of hypertension. The aim of this study was to assess urinary excretion of THP (U-THP) in healthy and hypertensive elderly patients. Fifteen young healthy subjects (YHS), 15 young hypertensive patients (YHT), 15 older normotensive (>60 years) subjects (OHS) and 31 older (>60 years) hypertensive patients (OHT) were examined. In all subjects 24-h urinary volume (UV), U-THP and creatinine (U-Cr), specific gravity of urine (U-SG), serum creatinine (S-Cr), and mean arterial pressure (MAP) were assessed. THP urinary excretion was significantly decreased in normotensive elderly patients, but not in hypertensive ones. Higher U-THP in the elderly hypertensive as compared with the elderly normotensive patients seems to be more the consequence than the cause of arterial hypertension.

Adolescent↗
PubMed1998

Tamm-Horsfall protein isolated from urine of pregnant and non-pregnant women has similar oligosaccharides.

BACKGROUND: Differences in the immunosuppressive activity of Tamm-Horsfall protein and uromodulin claimed by many investigators are controversial. They have been ascribed to variations in the carbohydrate moiety. METHODS: We isolated urinary glycoproteins from 18 non-pregnant (THP) and 12 pregnant (UM) women using the same method (adsorption on diatomaceous earth) and performed comparative studies including monocarbohydrate analysis and tests of reactivity with lectins, cytokines and serum proteins. RESULTS: No significant differences in monocarbohydrate content or in the reactivity with specific lectins were found between the native urinary glycoproteins or between their proteolytic products. Also, in Sda+ and Sda- donors monitored throughout pregnancy and after delivery, no time-dependent differences in the sugar moiety were observed. We found that THP/UM from Sda+ donors reacted more strongly with PHA-L than THP/UM from Sda- donors, providing further evidence for carbohydrate variations between Sda+ and Sda- determinants. No differences between THP and UM in terms of their reactivity with recombitant human interleukin (rhIL) 1 alpha, recombinant human tumour necrosis factor (rhTNF) alpha and some serum proteins were found, indicating similar biological activity. CONCLUSION: The results of our carbohydrate analysis together with previous data on amino acid analysis indicate that Tamm-Horsfall protein and uromodulin are identical or very similar glycoproteins.

Adult↗
PubMed1997

[The influence of uncomplicated pregnancy on the function of particular nephron segments].

There was determined excretion albumins, beta 2-microglobulin and Tamma-Horsfall's protein with urine in 50 healthy pregnant women just before delivery and in 15 healthy non pregnant women. There was established uncreased excretion of albumin and beta 2-microglobulin with urine in healthy pregnant women, however decreased excretion Tamma-Horsfall's protein. The results of the examinations suggest the function change particular nephron's section in physiologic pregnancy. The knowledge of this changes have significant meaning for proper results interpretation of index examinations activity particular nephron's section in healthy pregnant women.

Adult↗
PubMed1997

[The activity of renal glomeruli and tubules during uncomplicated pregnancy: influence of delivery on urinary excretion of albumin, beta 2-microglobulin and Tamm-Horsfall protein].

There were examined the influence the various types of delivery (spontaneous, oxytocin induced, cesarean section) on the value of albumin's (marker of glomerular activity), beta 2-microglobulin's (beta 2-M) (marker of activity proximal renal tubule) and Tamm-Horsfall's protein (pTH) (as a marker of activity distal's renal tubule) excretion in 50 healthy pregnant women without clinical symptoms of nephropathy. There were also estimated creating concentration in plasma and value of creating excretion in urine before and after delivery in every women. There were stated a little increase of albumin and beta 2-microglobulin but proper excretion (pTH) in urine in every group of pregnant women. There were estimated increase of albumin and pTH excretion in urine after spontaneous delivery, oxytocin inducted or cesarean section, particularly in women after cesarean section. The type of delivery had not an effect on the beta 2-microglobulin's urine excretion. The results of conducted researches suggest occurrence of functional changes in both renal glomeruli and tubules in physiologic pregnancy. The type of delivery reveals differentiated in quantity's respect the influence on albumin's and pTH excretion, but it has not an effect on beta 2-microglobulin's urine excretion.

Adult↗
PubMed1997

[Hypertension treatment in patients with diabetes].

Diabetic patients have an increased risk of hypertension. The coexistence of diabetes and hypertension causes higher morbidity and mortality in this population. In spite of differences between pathophysiology of hypertension in diabetes type I and type II, the antihypertensive treatment retards or prevents the appearance of overt diabetic nephropathy and reduces cardiac risk in both insulin-dependent and non-insulin-dependent diabetic subjects. Treatment of hypertension in both type I and type II diabetes comprises nonpharmacological and pharmacological interventions. Converting-enzyme inhibitors and certain calcium antagonists seems to be first line agents to preserve renal function and lower of the cardiac risk in patients with diabetes and hypertension.

Angiotensin-Converting Enzyme Inhibitors↗
PubMed1997

[Does a relationship exist between urinary excretion of Tamm-Horsfall protein and electrolytes in patients with diabetes type I and II without diabetic nephropathy?].

Studies were performed in 22 patients with diabetes mellitus type I, in 19 patients with diabetes type II, and in 15 healthy subjects. In all subjects the following parameters were assessed: creatinine clearance and 24 h urinary excretion of THP, albumin, sodium, potassium, and calcium. All studies were performed twice: first after 3 days on a normal sodium diet and subsequently after 3 days of sodium restriction (30-40 mmol Na/24 h). Urinary excretion of sodium was significantly lower in patients with diabetes mellitus type II than in healthy subjects. In patients with diabetes mellitus type I urinary excretion of sodium was significantly lower than in controls only after sodium restriction. No significant correlation was found between urinary excretion of THP and electrolytes. Results obtained in this study do not confirm presence of any relationship between urinary excretion THP and electrolytes.

Adult↗
PubMed1996

Alterations of Tamm-Horsfall protein immunoreactivity after partial desialylation and deglycosylation.

The role of the carbohydrate moiety of Tamm-Horsfall protein (THP) was investigated by studying the effect of partial enzymatic desialylation and deglycosylation on its immunoreactivity. In our experiments primarily sialic acid alpha 2,6 linked to a galactose was split off, resulting in a 14% increase of immunoreactivity. It was increased up to 21% by further removal of the sugar residues. We also found that complexes composed of THP and human serum albumin, immunoglobulin G or transferrin reacted with anti-THP antibodies. When these complexes were formed with desialylated or deglycosylated THP an essential increase of this reactivity (about 25%) occurred. Our studies indicate that modifications of the carbohydrate moiety of THP (which might occur in vivo by changes in the glycosylation process e.g. in pathologic conditions) lead to increased exposure of THP to the immune system.

Animals↗
PubMed1995

Abnormal function of renal tubules in patients with simple renal cysts.

The present study aimed to assess the function of proximal and distal tubules in patients with simple renal cysts. Thirty-one patients with simple renal cysts and 10 healthy subjects were examined. Based on the cyst fluid/plasma sodium ratio, 25 cysts were found to be of proximal origin and 6 of undetermined origin. In all patients cyst fluid and plasma concentrations of beta-2-microglobulin (beta-2-MG), sodium, potassium, calcium, phosphorus and total protein were assessed. Urinary excretions of sodium, potassium, calcium, phosphorus, beta-2-MG and Tamm-Horsfall protein (THP) were also estimated. Fractional excretion of beta-2-MG was calculated. The concentrations of beta-2-MG in fluid obtained from cysts of proximal origin were significantly higher than in fluid from cysts of undetermined origin (2.26 +/- 0 36 vs. 0.65 +/- 0.13 mg/l, p = 0.0004). Concentrations of THP (6.85 +/- 1.21 vs. 3.14 +/- 1.06 micrograms/ml, p < 0.05), and potassium (4.39 +/- 0.07 vs. 3.13 +/- 0.44 mmol/l, p < 0.05) were also higher in fluid from proximal cysts than in fluid from cysts of undetermined origin. Plasma beta-2-MG concentration was significantly higher in patients with proximal and undetermined cysts than in the control group (4.35 +/- 0.34 and 4.11 +/- 0.74 vs. 1.89 +/- 0.1 mg/l, p < 0.001). Urinary beta-2-MG excretion was also significantly higher in both groups of patients than in healthy subjects (474.8 +/- 165.9 and 346 +/- 94 vs. 100.2 +/- 19.6 micrograms/24 h, p < 0.05). Urinary THP excretion was significantly higher in patients with proximal cysts than in healthy subjects (31.0 +/- 5.1 vs. 16.3 +/- 2.5 mg/24 h, p < 0.05). From the results obtained in this study it seems that patients with simple renal cysts of proximal origin are characterized by abnormal tubular handling of beta-2-MG, and calcium and THP excretion. Thus, in patients with proximal cysts presence of a latent renal tubulopathy seems to be likely.

Adult↗
PubMed1994

Renal clearance of endogenous erythropoietin in patients with proteinuria.

Recent data indicated the importance of urinary losses of erythropoietin (Epo) in the pathogenesis of anaemia in patients with nephrotic syndrome. In the present study we aimed to investigate plasma and urinary Epo levels and their renal handling in relation to beta 2-microglobulin (beta 2m), sodium metabolism and the renin-angiotensin-aldosterone system (RAAS), respectively, in patients with sub-nephrotic range proteinuria (SNP), microalbuminuric diabetics and hypertensives, and in healthy subjects studied on a standardized diet containing 120 mmol sodium and 70 g protein per day. We found that patients with SNP were characterized by lower plasma levels of Epo than healthy subjects but no differences were found in urinary excretion of Epo, endogenous Epo clearance and its fractional excretion (FEEpo). There were no differences between groups in FE beta 2m and FENa and plasma aldosterone levels but plasma renin activity was higher in patients with SNP than in the controls. No relationships were found between Epo levels and activity of the RAAS and sodium metabolism, respectively. Our data suggest that lower levels of plasma Epo in patients with SNP and normal renal excretory function are not due to urinary losses of Epo but rather to the decreased production/degradation ratio.

Adult↗
PubMed1994

Effect of isobaric hyperoxemia on erythropoietin secretion in hypertensive patients.

We assessed the influence of hyperoxemia on erythropoietin secretion in patients with various etiological forms of arterial hypertension (essential, n = 15; renoparenchymal, n = 16; renovascular, n = 15) and in 15 healthy subjects. On the first day of the study, blood was withdrawn at 1-hour intervals for the estimation of erythropoietin during a total of 6 hours and at 2-hour intervals for the assessment of PO2. Three days later the same parameters were assessed again at identical time intervals, but the subjects were breathing pure oxygen during the first 2 hours. Breathing with pure oxygen resulted in a significant increase of blood PO2 (184.85 +/- 4.47 versus 85.92 +/- 2.28 in essential, 185.21 +/- 5.52 versus 84.55 +/- 3.04 in renoparenchymal, and 181.7 +/- 3.14 versus 87.49 +/- 2.25 in renovascular hypertension groups and 189.84 +/- 5.2 versus 85.89 +/- 1.73 mm Hg in healthy subjects; P < .001 in all groups). Baseline plasma erythropoietin was not different among the groups (29.33 +/- 4.14 in essential, 24.56 +/- 3.09 in renoparenchymal, and 27.77 +/- 3.29 in renovascular hypertension groups and 24.23 +/- 2.70 mU/mL in the control group). The pattern of erythropoietin decline was different in the groups of hypertensive patients. In patients with essential hypertension, unlike in healthy subjects and patients with other etiological forms of arterial hypertension, only a very short-term suppression of erythropoietin levels was observed during hyperoxemia. No significant changes in blood pressure during breathing with pure oxygen were found in any of the studied groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗