Immune complexes were precipitated by 3% polyethylene glycol (PEG) from 4 sera from albendazole-treated hydatid patients from Uruguay, and together with sheep hydatid cyst fluid were analysed by ion-exchange fast protein liquid chromatography (FPLC). FPLC-eluted fractions of serum preparations and cyst fluid were tested in an enzyme-linked immunosorbent assay for the detection of specific Echinococcus granulosus antigens and also for presence of specific immunoglobulin (Ig) M and IgG in the serum samples. Two peaks (nos 13 and 25) were found to have antigenic activity. Antigens were identified in both serum and cyst fluid preparation (fraction 25) after sodium dodecyl sulphate-polyacrylamide gel electrophoresis immunoblotting analysis, with approximate molecular masses 50 kDa and 62 kDa. Specific IgM antibody was also detected in the same FPLC fraction (no. 25) of PEG-precipitated serum complexes.

Adolescent↗

PubMedDate not supplied

Early language screening in City and Hackney: work in progress.

The paper describes the initial stages in the development of a screening instrument designed to identify children with impaired language development at the age of 2 1/2 years. The pilot study comprised 40 children seen for routine developmental surveillance at one of the Community Health Centres in City and Hackney District Health Authority. The screen was found to be both sufficiently sensitive and sufficiently specific to warrant further investigation. The discussion highlights a number of pertinent issues which have arisen from the study and which the authors consider to be of relevance to others considering the development of equivalent procedures.

Child, Preschool↗

PubMedDate not supplied

Anti-C as a naturally-occurring antibody.

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Arthroplasty↗

PubMedDate not supplied

New labour, new NHS?

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Health Care Reform↗

PubMedDate not supplied

Assessing smoke-free workplaces in Wisconsin municipal and county government buildings, 1997.

Passive smoke has been shown to cause cancer and heart disease in humans. We surveyed all Wisconsin cities and villages in 1997 to determine their clean indoor air policies in government-owned buildings. By 1997, 62% of cities and villages and 100% of counties had enacted a clean indoor air ordinance or policy. Smoking is completely banned in 26% of all city and village buildings and 49% of county buildings in the state. This survey shows that an increasing proportion of government worksites in Wisconsin are enacting clean indoor air policies to protect their employees and the public from the health effects of passive smoke.

Humans↗