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Biomedical subjects

J Dankert

Publications and source records attributed to J Dankert.

299 records · Page 17Linked to original sources
PubMed1975

Isolation of hepatitis B surface antigen (HBsAg) by affinity chromatography on antibody-coated immunoadsorbents.

Hepatitis tb surface antigen (HBsAg) was isolated from human serum by two steps of affinity chromatography on antibody-coated gels. HBsAg-positive serum was passed through a column packed with guinea pig anti-HBsAg antibodies covalently bound to CNBr-activated beaded agarose gel. The majority of non-specifically bound proteins was removed by washing the gel with increased concentrations (0.5 M) of NaCl in Tris buffer. Elution of the specifically bound HBsAg was carried out with 3 M NaSCN. Residual normal human serum proteins present in the eluate were removed by passing the partially purified HBsAg through an immunoadsorbent coated with rabbit antibodies directed against human serum proteins. After this treatment normal human serum proteins could no longer be demonstrated by passive hemagglutination in the isolated HBsAg. Cross-reactions between HBsAg and normal human serum proteins could not be demonstrated. Both antibody-coated immunoadsorbents could be used over ten times without significant loss of their binding capacity.

ABO Blood-Group System↗
PubMed1970

Bactericidal properties of Tego 103 S and Tego 103 G.

The bactericidal activity of Tego 103S was compared with that of chlorhexidine in ethanol, and Tego 103G with Halamid. The activity was determined on Pseudomonas aeruginosa, Staphylococcus aureus and Achromobacter anitratus by various methods. Tego 103S in 1% solution was less active than chlorhexidine 0.5% in 70% ethanol, and Tego 103G in 1% solution was less active than Halamid 0.3%. The presence of serum did not noticeably influence the activity of the Tego preparations.

Alcaligenes↗
PubMedDate not supplied

High prevalence of Enterocytozoon bieneusi infections among HIV-positive individuals with persistent diarrhoea in Harare, Zimbabwe.

Infection with the microsporidian parasite Enterocytozoon bieneusi may be a major cause of prolonged diarrhoea in individuals also infected with human immunodeficiency virus (HIV). The parasite has been reported from Europe, Australia and the Americas, with a prevalence of 7-29%. Faecal specimens were obtained from 202 adults and 106 children in Harare, Zimbabwe, all of whom were in hospital and had diarrhoea. HIV serology was available for 119 adults: 106 were HIV seropositive. There were clinical grounds for suspecting HIV infection in 23 of the remaining patients. E. bieneusi was identified in specimens from 13/129 patients (10%) for whom HIV infection was indicated by serology and/or clinical signs, 1/60 patients (2%) of uncertain HIV status, and 0/13 seronegative patients. 18/106 children were HIV seropositive and 12 were not; HIV serology was not available for the remainder, but 19 were strongly suspected of being infected with HIV on clinical criteria. E. bieneusi was not detected in samples from any child. As is common in Zimbabwe, the prevalence of other parasites in faecal specimens was low and, amongst patients with proven or suspected HIV infection, E. bieneusi was the most prevalent parasite identified, particularly in patients with diarrhoea of over 4 weeks duration.

Adolescent↗
PubMedDate not supplied

Prediction of flucytosine-induced thrombocytopenia using creatinine clearance.

AIMS: To evaluate and investigate the efficacy and accuracy of an earlier derived relationship between thrombocyte nadir and mean creatinine clearance in order to make it possible to predict flucytosine (5-FC)-related thrombocytopenia. METHODS: The earlier derived relationship between thrombocyte nadir and mean creatinine clearance is validated using a patient population of 35 intensive care unit (ICU) patients. Patients have to meet the inclusion criteria as defined in the initial study (i.e., developing thrombocytopenia during 5-FC treatment or remaining within the normal thrombocyte range; individual pharmacokinetic parameters within certain limits; a minimum of three measured 5-FC serum levels). RESULTS: Of a total of 35 patients, 11 are eligible since they meet the inclusion criteria as defined in the initial study. A significant relationship between the observed and predicted thrombocyte nadir values can be derived: Y(pred) = 0.608.X(obs) + 24. 330 (r = 0.615; t(obs) = 2.337; t at p < 0.05 and 9 degrees of freedom = 2.262). The predicted value of the thrombocyte nadir corresponds with a mean prediction error (bias) of -18.3 (95% CI -45.9; 9.4) and a root mean squared prediction error (precision) of 48.3 (95% CI 23.9-63.9). CONCLUSION: The earlier derived relationship between thrombocyte nadir and mean creatinine clearance is accurate and precise. However, due to the strict inclusion criteria used to derive and validate this relationship, it cannot be applied to all 5-FC-treated patients.

Aged↗