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Biomedical subjects

J Daniels

Publications and source records attributed to J Daniels.

At least 55 records · Page 3Linked to original sources
PubMed1992

Multidisciplinary preoperative assessment and late function in dysvascular amputees.

During a three-year period, 95 adults were treated by lower-extremity amputations for peripheral vascular insufficiency. A multidisciplinary team for presurgical evaluation determined all to be potentially independent walkers with a prosthesis. Patients were graded for level of independent walking before amputation and again at a minimum two-year follow-up examination using a seven-level functional grading system. Seventy-six of 90 patients (84%) ambulated within one functional level of their preamputation status. This return to preamputation level of function was maintained whether comparing preamputation functional level or surgical amputation level. Prosthetic use increased and the use of walking aids decreased with increased functional ambulation levels, but did not correlate with surgical levels. Peripheral vascular insufficiency patients can maintain walking independence. Multidisciplinary presurgical evaluation helps selection of the amputation level and correct prosthetic limb fitting.

Adult↗
PubMed1992

Running economy of elite male and elite female runners.

Twenty female and 45 male middle and long-distance runners, in training for the U.S. Olympic Trials, served as subjects. Ninety percent of both men and women subjects reached the Trials; eight women and 12 men qualified for the Olympic Games and five won medals. Each subject completed a VO2max and a series of submax treadmill runs, for the purpose of comparing heart rate (HR), VO2, and blood lactate (HLa) among men and women and among runners of various event specialties. Results showed the men to be taller, heavier, to have a lower six-site skinfold sum and a higher VO2max, than the women (P less than 0.05); there was no difference in age. When compared in running economy, men used less oxygen (ml.min-1.kg-1) at common absolute velocities, but VO2 (ml.km-1.kg-1) was not different between men and women at equal relative intensities (%VO2max). When men and women of equal VO2max were compared, the men were significantly more economical, using any method of comparison. Also, when comparisons of men and women of equal economy were made, it was found that the men had an even greater advantage over the "matched" women subjects than the mean VO2max comparison using all subjects. In looking at the SD (800-/1500-m runners), MD (3-K/5-K/10-K runners) and LD (marathon runners), it was found that the SD runners used the least oxygen (ml.min-1.kg-1) at speeds of marathon race pace and faster, but not at slower speeds. Men and women responded similarly in this regard. Running economy data for speeds slower than typical race paces, tended to show the LD runners to be most economical, suggesting that the speeds over which runners are tested plays an important part in determining which subjects are the most economical. It was concluded that at absolute running velocities, men are more economical than women, but when expressed in ml.km-1.kg-1 there are no gender differences at similar relative intensities of running. Also, when men and women of equal VO2max or equal economy are matched, the men show a better aerobic profile. It is recommended that economy data be collected up to speeds equal to over 90% VO2max.

Adult↗
PubMed1991

Cytology of treated and minimal Pneumocystis carinii pneumonia and a pitfall of the Grocott methenamine silver stain.

To clarify the role of foamy alveolar casts (FACs) in the diagnosis of Pneumocystis carinii pneumonia (PCP), we retrospectively reviewed Papanicolaou (Pap)- and Grocott methenamine silver (GMS)-stained slides from 205 bronchial specimens submitted for suspected opportunistic lung infection. FACs containing sporozoites were seen in 86 cases, all with positive GMS. FACs were absent in 119 cases with negative GMS. In patients previously treated for PCP, Pap showed FAC-like material which lacked sporozoites. GMS demonstrated clumped degenerated Pneumocystis carinii (PC) cysts. In 17 GMS-positive cases there were no FACs on Pap due to sampling error and low burden of organisms. Morphologic features of macrophages in these cases can suggest the presence of PC. When FACs are lacking, a special stain is required. When GMS is used, the control must contain PC to avoid a false negative GMS. The inconsistent uptake of GMS by PC is a pitfall which is distinct from sampling error and which to our knowledge has not been previously reported.

Bronchi↗
PubMed1991

Use of recovery VO2 to predict running economy.

The purpose of this study was to determine whether running economy (RE) could be predicted accurately using recovery VO2 values. Twelve runners (means VO2max = 61.9, SD 4.9 ml.kg-1.min-1) completed three treadmill RE sessions over a 2-week period. During each session, subjects performed three 6-min runs at 69%, 78%, and 87% VO2max. RE was calculated from a single 2-min gas collection during the last 2 min of running. Immediately following each run, recovery VO2 data obtained during randomly assigned 15-s, 20-s, or 25-s gas collections were used to predict exercise VO2. Correlations and mean absolute percentage variation (% VAR) between actual and predicted VO2 at each relative intensity and recovery period are reported. Although the relationship between actual and predicted VO2 was significant and more pronounced at higher exercise intensities, the overall magnitude of the association was low to moderate (r range = 0.50-0.81). The range of % VAR between actual and predicted aerobic demands also obscured marked underprediction (-6.5% to -12.5%) and overprediction (+10.1% to +17.4%) of actual VO2 in some subjects. These data suggest that 15-, 20-, and 25-s recovery VO2 values do not correlate strongly with steady-state VO2, nor do they adequately account for variation in individual economy profiles.

Adult↗
PubMed1991

The nutritional habits of young adolescent swimmers.

Dietary food records from adolescent male and female swimmers participating in a national developmental training camp were analyzed for nutrient density. The mean caloric intake was 5,221.6 kcal for males and 3,572.6 kcal for females. The distribution of calories between carbohydrates, protein, and fat was not ideal for athletes trying to optimize performance. These young swimmers consumed too much fat and not enough carbohydrate. They consumed more than the RDA of vitamins A and C, and thiamine, riboflavin, and niacin; however, some concern is expressed for females who did not meet the RDA for calcium and iron. This study shows that although a group of adolescent swimmers may be consuming enough nutrients, individual swimmers may have very poor dietary habits and thus may not be providing adequate fuel or nutrients for optimal training or performance.

Adolescent↗
PubMed1991

A closely linked DNA marker for facioscapulohumeral disease on chromosome 4q.

Close linkage of a hypervariable DNA probe on chromosome 4q (pH30, locus D4S139) has been found with the locus for facioscapulohumeral disease. Three recombinants were identified in a total of 140 meioses, giving a maximum lod score of 36.77 at a recombination fraction of 0.02. All but two of the families studied proved informative with this probe; all informative families showed evidence of linkage (except one family with a single scorable meiosis), making genetic heterogeneity unlikely from our data. The close linkage and highly informative nature of the probe will make it suitable for clinical application in presymptomatic and prenatal diagnosis. We have also confirmed loose linkage with the marker (Mfd22, locus D4S171) used to establish the initial assignment of the disorder to chromosome 4.

Chromosome Mapping↗
PubMed1991

Treatment of superior vena cava thrombosis with recombinant tissue type plasminogen activator.

Thrombotic occlusion of the superior vena cava is an uncommon but serious complication of chronic indwelling venous catheters. Several reports have shown thrombolytic therapy with intravenous streptokinase or urokinase to be effective in the treatment of this condition. We report a case of superior vena cava thrombosis in a 53-year-old woman receiving chemotherapy for breast carcinoma through a subcutaneously implanted venous access catheter who was successfully treated with peripheral infusion of recombinant tissue type plasminogen activator (rtPA).

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1989

Bovine brucellosis: evaluation of field sera by a competitive and superimposable ELISA utilising a monoclonal antibody against Brucella abortus lipopolysaccharide.

With the aid of a horseradish peroxidase (HRP) tagged monoclonal antibody against smooth lipopolysaccharide from Brucella abortus (Bruce 1), a competitive and superimposable ELISA test procedure for bovine brucellosis has been evaluated for its ability to discriminate between Strain 19-vaccinated (S19-Vacc) and Biotype 1-infected (B1-Inf) cattle. In the competitive assay, all sera from S19-Vacc animals competed effectively against HRP-Bruce 1 (low HRP activity), while 10 out of 40 B1-Inf animals competed less effectively with Bruce 1 (high HRP activity). Successful competition by cattle antibodies would result in an increased proportion of cattle Igs binding to the assay antigen. This was confirmed by superimposing an alkaline phosphatase conjugated rabbit anti-cattle Ig after the competitive ELISA had been completed. With the superimposable assay, alkaline phosphatase activity was correspondingly high for S19-Vacc animals, and low for 36 out of 40 B1-Inf animals. The superimposable ELISA had therefore improved the discriminatory capabilities of the assay procedure from 75% to 90%.

Animals↗
PubMed1989

GLQ223: an inhibitor of human immunodeficiency virus replication in acutely and chronically infected cells of lymphocyte and mononuclear phagocyte lineage.

GLQ223 is a highly purified, formulated preparation of trichosanthin, a 26-kDa plant-derived ribosome-inactivating protein with potent inhibitory activity against human immunodeficiency virus (HIV) in vitro. The compound produced concentration-dependent inhibition of HIV replication in acutely infected cultures of T-lymphoblastoid cells (VB cell line). Treatment with GLQ223 selectively reduced levels of detectable viral proteins compared to total cellular protein synthesis and produced a selective decrease in levels of viral RNA relative to total cellular RNA in acutely infected cells. Substantial inhibition of viral replication was observed at concentrations of GLQ223 that showed little inhibition of parallel uninfected cultures. Selective anti-HIV activity was also observed in cultures of primary monocyte/macrophages chronically infected with HIV in vitro. When freshly drawn blood samples from HIV-infected patients were treated with a single 3-hr exposure to GLQ223. HIV replication was blocked for at least 5 days in subsequently cultured monocyte/macrophages, without further treatment. The anti-HIV activity of GLQ223 in both acutely and chronically infected cells and its activity in cells of both lymphoid and mononuclear phagocytic lineage make it an interesting candidate as a potential therapeutic agent in HIV infection and AIDS.

Antigens, Viral↗
PubMed1989

Pigeon breeder's disease: the effect of antigen-specific antibodies on in vitro T cell responses of normal blood lymphocytes.

The effect(s) of affinity-purified antibodies to pigeon serum immunoglobulins on normal T cell responses in vitro was measured. Our results show that the antibodies partially inhibited the concanavalin-A-induced proliferation of normal lymphocytes (32% at 10 micrograms/ml concentration; p less than 0.001), whilst they had no significant effects at equivalent doses on the phytohemagglutinin-induced response. A study of the kinetics of this inhibition revealed that the antibodies exerted their effect(s) within the first 24 h of culture (p less than 0.001); this is probably due to their interference in early events intimately involved in the de novo synthesis and expression of activation antigens such as HLA-DR and Tac: antibody-treated cultures expressed 51.6 and 41.4% less HLA-DR and Tac, respectively. Two-colour immunofluorescence analysis showed that the specific antibodies bind to a subset of CD8+ cells only.

Alveolitis, Extrinsic Allergic↗
PubMed1988

Cholera toxin partially inhibits the T-cell response to phytohaemagglutinin through the ADP-ribosylation of a 45 kDa membrane protein.

This study examines the influence of cholera toxin (CT) on T lymphocyte activation by the mitogenic lectin phytohaemagglutinin (PHA). CT suppressed lectin-induced [3H]thymidine uptake in a dose-dependent fashion and acted synergistically with PHA in the generation of intracellular cyclic AMP. The toxin was assumed to act on Gs, because it also stimulated ADP-ribosylation of a 45 kDa membrane protein in vitro; no additional substrates were seen. The inhibitory effect of the adenylate cyclase/cyclic AMP pathway was shown to be directed at a concomitant stimulatory pathway, namely inositol phospholipid turnover. Lectin-stimulated 32P incorporation into both phosphatidylinositol as well as its 4,5-biphosphate derivative was depressed in the presence of CT or exogenous dibutyryl cyclic AMP. This, in turn, was associated with reduced activation of C-kinase as determined by decreased lectin-induced translocation from the cytosol to the surface membrane. These results indicate that Gs probably acts as a transducer between the PHA receptor and adenylate cyclase and may give rise to an exaggerated adenylate cyclase response in the presence of CT. It would seem as if reduction in inositol phospholipid turnover is related to the elevation of cyclic AMP rather than a CT effect on a putative transducer which acts directly on phospholipase C. Our study does not exclude the existence of non-CT-sensitive transducers in this capacity.

Adenosine Diphosphate↗
PubMed1987

C-kinase activity in normal B cells treated with Staphylococcus aureus, Cowan strain I, and phorbol ester: response differences in a patient with prolymphocytic leukaemia.

Ca+2/phospholipid-dependent kinase (C-kinase) activity is intimately involved with the B-cell response after ligation of its mIg receptor. Here, we extend previous findings with anti-mIg by showing that polyclonal stimulation with Staphylococcus aureus, Cowan strain I (SAC), could also translocate C-kinase from the cytosol of intact, normal human B lymphocytes. This stimulus could not, however, induce redistribution of enzyme in a prolymphocytic leukaemia (PLL) B-cell population. Despite a normal density of mIg receptors on the latter cell type, PPL cells could not be stimulated to proliferate, express interleukin-2 (IL-2) receptors, and differentiate under the influence of SAC, even in the presence of exogenous IL-2. Normal B cells could respond appropriately to the same stimuli. PLL cells had a normal content of C-kinase activity and the enzyme could be translocated under the influence of the specific agonist, 12-0-tetradecanoyl phorbol-13-acetate (TPA). The same agent could induce cellular proliferation and differentiation in PLL cells, showing that its terminal C-kinase dependent pathways were intact. We conclude that the mIg receptor mechanism of this PLL population could not activate C-kinase appropriately.

Adult↗
PubMed1986

Height and weight of Southeast Asian preschool children in Northern California.

Anthropometric data were obtained from 526 Southeast Asian preschool children during 1980-84. Length of time in the United States averaged 21.8 months. Mean weights and heights were substantially below the National Center for Health Statistics (NCHS) 50th percentile. Although 39 per cent of children were below the 5th percentile of height for age, only 7 per cent were below the 5th percentile of weight for height. Rates of weight and height gain were similar to reference values, indicating adequate growth after arrival in the US.

Asia, Southeastern↗
PubMed1986

Ruptured intracranial aneurysms during pregnancy. A report of four cases.

Four patients presented with ruptured intracranial aneurysms during pregnancy. Problems encountered during the management of these cases included delayed diagnosis, obstructive hydrocephalus, cerebral ischemia due to vasospasm and recurrent subarachnoid hemorrhage. Recent advances in the management of ruptured intracranial aneurysms, including early computerized tomographic scanning, intravascular volume expansion and induced hypertension for the management of cerebral vasospasm, and the timely obliteration of the aneurysm are applicable to the subarachnoid hemorrhage patient even if her condition is complicated by pregnancy.

Adolescent↗
PubMed1985

Lectin-binding to hemocytes of Mytilus edulis.

Lectin receptors for WGA, Ricinus 60, Ricinus 120, and for the agglutinin from the albumin gland of Helix pomatia were detected on the surface of Mytilus hemocytes by an agglutination assay. When hemocyte monolayers on slides were incubated with these lectins, strong rosette formation was obtained after addition of human erythrocytes. The lowest lectin concentration which caused formation of rosettes was determined to be 5 micrograms/ml of WGA or of Helix agglutinin, and 20 micrograms/ml of Ricinus 60 or 40 micrograms/ml of Ricinus 120, respectively. In contrast to these results, no binding of indicator cells to Mytilus hemocytes occurred with various other lectins, including Con A.

Agglutination Tests↗