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Biomedical subjects

J Dagan

Publications and source records attributed to J Dagan.

30 records · Page 2Linked to original sources

A simple method for determining isotope uptake in disseminated metastatic thyroid disease.

A simple method for determining isotope uptake in disseminated metastatic thyroid disease is described. This method can be adopted by a nuclear medicine department which has no on-line computer for acquisition and handling of images from a gamma camera. The equipment needed is the gamma camera, a storage device for images (analog or digital), and an option to define regions of interest and their count rate. Today this option is available in any standard gamma camera. The method is outlined by a specific example of a clinical case.

Female↗

The carotid and ECG pulses as indices for nuclear cardiography imaging.

Nuclear cardiography has emerged from nuclear medicine to help the cardiologist in assessing the heart's performance. One of the examinations practiced is imaging of the cardiac muscle at its two extreme phases: End-Systole (ES) and End-Diastole (ED). Images of those two phases of the cardiac cycle may provide information for the calculation of Ejection Fraction (EF) and the asynergy and dysynergy of the heart muscle.

Carotid Arteries↗

A newly designed whole microplate automatic harvester for lymphocyte stimulation assays.

A unique automated sampling manifold designed to recover cells grown in standard 96 well microplates from their culture medium is described. Cells are recovered and washed on fiber glass filter discs. Incorporation of radioisotopes into cells, can then be measured by appropriate counting of the filter discs. Typical applications include termination of mixed lymphocyte cultures, assays of mitogen stimulation of lymphocytes and antigen-specific lymphocyte transformation and assays of interferon activity. The harvester can also be used in other biological systems where collection and washing of precipitates is desired.

Autoanalysis↗

Soft tissue stretching with magnets.

Various clinical conditions require soft tissue elongation that can later host a bone graft. The possibility of stretching the soft tissues by magnetic force was tested on the forearm of dogs. The results are encouraging, provided certain precautions are taken and the pull exerted is not too great.

Amputation Stumps↗

A long unidentifiable extra chromosomal segment--a possible duplication of human 7q.

Limitation of current techniques in identifying extra chromosomal segments arising de novo is illustrated by a putative case of a duplication of the long arm of chromosome 7. The propositus, demonstrating multiple congenital anomalies and severe mental retardation, had a large extra segment of chromatin on chromosome 7q that was absent in his parents. The banding pattern of this segment resembled that of the long arm of chromosomes 7, 8, or 9. Various procedures indicated that the additional material did not include the secondary constriction of 9q. The phenotype of the propositus did not fit well with that of trisomy 8.

Abnormalities, Multiple↗

Partial trisomy D: a diagnostic and cytogenetic dilemma.

An 18-month-old proposita with psychomotor retardation and other congenital abnormalities is presented. Chromosomal analysis of both parents proved normal. However, the karyotype of the proposita contained 47 chromosomes in both lymphocytes and cultured fibroblasts. The marker chromosome proved to be a deleted No. 14 or 15. Comparison of the reported cases of partial trisomy D indicates that a definitive clinical syndrome is not apparent in either case.

Chromosome Deletion↗

Cytogenetic investigations in families with ataxia-telangiectasia.

Chromosomal studies were performed on peripheral blood lymphocytes and cultured skin fibroblasts from five Israeli-Moroccan families with ataxia-telangiectasia. A total of 24 individuals, including seven propositi, was investigated. Among the probands, significantly elevated rates of chromosome damage were observed in both blood and skin. Skin fibroblasts of affected individuals showed several orders of magnitude more chromosome breakage than lymphocytes. Increased rates of chromosome damage were also observed in the fibroblasts of some phenotypically normal family members (obligate heterozygotes and sibs) when compared to normal controls. An apparent abnormal clone of cells, possessing a large acrocentric marker chromosome (14q+), was observed in varying proportions among cells of all the propositi (2-5% of lymphocytes; 1-9% of fibroblasts).

Ataxia Telangiectasia↗

Gas scavenging during bronchoscopy under general anesthesia.

Prevention of exposure of the endoscopist to high levels of anesthetic gases during bronchoscopy was attempted experimentally in dogs by a scavenging system. Results were compared with exposure during the conventional technique of anesthetic gas administration for clinical bronchoscopy using the rigid open ventilating bronchoscope. The scavenging system consisted of a vacuum pump applied to the open ventilating rigid bronchoscope sidearm connection during intratracheal administration of nitrous oxide, , oxygen, and halothane gas mixture. Gas samples were taken from the trachea, the proximal end of the bronchoscope, and the endoscopist's breathing zone, and analyzed by gas chromatography. Findings indicate that halothane anesthesia for bronchoscopy administered by conventional techniques is a source of air pollution in the operating room and exposes the endoscopist to subanesthetic levels of halothane that may affect psychomotor functioning. The use of the gas scavenging system lowered the concentrations of halothane and nitrous oxide at the endoscopist's breathing zone to a level at which inhalation for short periods has no clinical effects, while the concentrations of the anesthetics and oxygen in the trachea were maintained at a satisfactory level.

Air Pollutants, Occupational↗

Transient neonatal diabetes mellitus in a child with invdup(6)(q22q23) of paternal origin.

An association between the rare condition of transient neonatal diabetes mellitus and either uniparental disomy for chromosome 6 or dup(6)(q22q23) raised the assumption that in this location on chromosome 6 there is an imprinted gene. We diagnosed diabetes that developed in a baby girl immediately after birth and resolved after 7 weeks of insulin treatment. Due to some minor dysmorphic features, we investigated her karyotype and identified invdup(6)(q22q23). The duplication spans at least 10 cM including the DNA sites DS270,S314,S1684 and S310. This case further supports the assumption that an imprinted gene exists on chromosome 6q22-23.

Child, Preschool↗