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Biomedical subjects

J D Moore

Publications and source records attributed to J D Moore.

At least 271 records · Page 15Linked to original sources
PubMed1996

1996 up & comers. Strategies for success.

They're bright, focused and aggressive. They're on a mission to improve the quality of America's healthcare provider organizations. They're MODERN HEALTHCARE's 1996 Up & Comers, a dozen healthcare professionals 40 and younger who are in the vanguard of the next generation.

Administrative Personnel↗
PubMed1996

A point mutation in the microtubule binding region of the Ncd motor protein reduces motor velocity.

Non-claret disjunctional (Ncd) is a kinesin-related microtubule motor protein in Drosophila that functions in meiotic spindle assembly in oocytes and spindle pole maintenance in early embryos. The partial loss-of-function mutant ncdD retains mitotic, but not meiotic, function. The predicted NcdD mutant protein contains a V556-->F mutation in the putative microtubule binding region of the Ncd motor domain. Here we report an analysis of the properties of recombinant Ncd and NcdD proteins. A GST-NcdD fusion protein translocated microtubules approximately 10-fold more slowly than the corresponding wild-type protein in gliding assays. The maximum microtubule-stimulated ATPase activity of an NcdD motor domain protein was reduced approximately 3-fold and an approximately 3-fold greater concentration of microtubules was required for half-maximal stimulation of ATPase activity, compared with the corresponding wild-type protein. The Km for ATP and basal rate of ATP turnover were, in contrast, similar for the NcdD mutant and wild-type Ncd motor domain proteins. Pelleting assays demonstrated that the binding of the mutant NcdD motor protein to microtubules was reduced in the absence of nucleotide, relative to wild-type. The reduced velocity of NcdD translocation on microtubules is therefore correlated with reductions in microtubule-stimulated ATPase activity and affinity of the mutant motor for microtubules. The characteristics of the NcdD motor explain its meiotic loss of function, and are consistent with partial motor activity of Ncd being sufficient for its mitotic, but not its meiotic, role.

Adenosine Triphosphatases↗
PubMed1996

Blood from a stone.

Whether due to the federal government's generosity in handing out waivers or the creativity of healthcare visionaries across the United States, Medicaid is assuming a variety of shapes, colors and sizes. One of the largest experiments is in California.

California↗