Stimulating circulation to end stasis in scleroderma.
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Adolescent↗
Biomedical subjects
J D Li
Publications and source records attributed to J D Li.
Presence of a cytochrome b-containing ferritin in Azotobacter vinelandii.
It has been demonstrated that a cytochrome b-containing ferritin is present in Azotobacter vinelandii. After DEAE cellulose chromatography and purification fractional precipitation by 50% of the saturated ammonium sulfate of the extract prepared from A. vinelandii cells, a hexagonal crystalline preparation is obtained. The protein contains 4--6% of nonheme iron. The protein molecule is made up of an electron dense iron core with a diameter of 70A and a protein shell with a diameter of 120A. The Fe core can be removed from the shell by the treatment with chelating and reducing agents. Electron micrographs and absorption spectra reveal that the protein shells are very similar before and after the removal of the core. The electrophoretic mobility and immunological properties of the Fe-free protein against the antibody of ferritin are very similar to those of the protein before the removal of the iron. From the above characteristics, it can be inferred that the protein belongs to ferritin. The protein part contains protoheme as prosthetic group and so it belongs to cytochrome b. Hence, the protein prepared from A. vinelandii is a kind of cytochrome b-containing ferritins. The possible role of the ferritin in biological nitrogen fixation is discussed in this paper.
Apoferritins↗
Light-dependent hydration of the space surrounding photoreceptors in the cat retina.
We have studied the effect of retinal illumination on the concentration of the extracellular space marker tetramethylammonium (TMA+) in the dark-adapted cat retina using double-barreled ion-selective microelectrodes. The retina was loaded with TMA+ by a single intravitreal injection. Retinal illumination produced a slow decrease in [TMA+]o, which was maximal in amplitude in the most distal portion of the space surrounding photoreceptors, the subretinal space. The light-evoked decrease in [TMA+]o was considerably slower and of a different overall time course than the light-evoked decrease in [K+]o, also recorded in the subretinal space. [TMA+]o decreased to a peak at 38 s after the onset of illumination, then slowly recovered towards the baseline, and transiently increased following the offset of illumination. It resembled the light-evoked [TMA+]o decreases previously recorded in the in vitro preparations of frog (Huang & Karwoski, 1990, 1992) and chick (Li et al., 1992, 1994) but was considerably larger in amplitude, 22% compared with 7%. As in frog, where it was first recorded, the light-evoked [TMA+]o decrease is considered to originate from a light-evoked increase in the volume of the subretinal space (or subretinal hydration). A mathematical model accounting for [TMA+]o diffusion predicted that the volume increase underlying the response was 63% on average and could be as large as 95% and last for minutes. The estimated volume increase was then used to examine its effect on K+ concentration in the subretinal space. We conclude that a light-dependent hydration of the subretinal space represents a significant physiological event in the intact cat eye, which should affect the organization of the interphotoreceptor matrix, and the concentrations of all ions and metabolites located in the subretinal space.
Animals↗
Calcium gradients and light-evoked calcium changes outside rods in the intact cat retina.
We have studied light-evoked changes in extracellular Ca2+ concentration ([Ca2+]o) in the intact cat eye using ion-sensitive double-barreled microelectrodes. Two prominent changes in Ca2+ concentration were observed that differed in retinal location. There was a light-evoked increase in [Ca2+]o, accompanied by brief ON and OFF transients, which was maximal in the inner plexiform layer and was not further studied. There was an unexpected sustained light-evoked decrease in [Ca2+]o, of relatively rapid onset and offset, which was maximal in the distalmost region of the subretinal space (SRS). [Ca2+]o in the SRS was 1.0 mM higher than in the vitreous humor during dark adaptation and this transretinal gradient disappeared during rod-saturating illumination. After correcting for the light-evoked increase in the volume of the SRS, an increase in the total Ca2+ content of the SRS during illumination was revealed, which presumably represents the Ca2+ released by rods. To explain the light-evoked [Ca2+]o changes, we used the diffusion model described in the accompanying paper (Li et al., 1994b), with the addition of light-dependent sources of Ca2+ at the retina/retinal pigment epithelium (RPE) border and rod outer segments. We conclude that a drop in [Ca2+]o around photoreceptors, which persists during illumination and reduces a transretinal Ca2+ gradient, is the combined effect of the light-evoked SRS volume increase, Ca2+ release from photoreceptors, and an unidentified mechanism(s), which is presumably Ca2+ transport by the RPE. The relatively rapid onset and offset of the [Ca2+]o decrease remains unexplained. These steady-state shifts in [Ca2+]o should have significant effects on photoreceptor function, especially adaptation.
Animals↗
Serum and egg yolk IgG antibody titers from laying chickens vaccinated with Pasteurella multocida.
Through determining the serum and egg yolk antibody titers in immunized laying hens to Pasteurella multocida regularly, the growth-decline trend of the egg yolk antibody levels was found to be similar to that of the serum antibody levels (r = 0.94), but the growth and decline of the egg yolk antibody seemed to be delayed 3-6 days compared with that of the serum antibody, and the egg yolk antibody titers were generally lower than those of the serum antibody (P < 0.01). Serum and egg yolk antibody levels declined 3 and 6 days, respectively, after booster immunizations. The higher the antibody levels were before booster immunization, the more they declined.
Animals↗