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Biomedical subjects

J D Klein

Publications and source records attributed to J D Klein.

125 records · Page 7Linked to original sources
PubMed1987

Pyoderma gangrenosum occurring at multiple surgical incision sites.

Ten weeks after subtotal colectomy and ileostomy for ulcerative colitis, a 16-yr-old girl developed wound drainage and back pain. Massive ulceration and skin separation occurred at the abdominal wound incision as well as at the incision sites of a previous central venous line. A diagnosis of pyoderma gangrenosum was made. High-dose steroid treatment induced prompt healing of the abdominal wound as well as the catheter sites. Pyoderma gangrenosum has rarely been seen in surgical wounds. To our knowledge, this is the first reported case of pyoderma gangrenosum occurring simultaneously in multiple surgical incision sites in a patient with ulcerative colitis.

Adolescent↗
PubMed1987

Effect of high temperature on calcium uptake by suspension-cultured pear fruit cells.

Uptake of Ca(2+) by suspension-cultured pear (Pyrus communis L. cv Passe Crassane) cells and protoplasts was significantly enhanced by exposure to 38 degrees C compared to 25 degrees C. The increased uptake was specific for Ca(2+) and was not due to cell wall binding. Tissues pretreated at 38 degrees C showed increased uptake even upon return to 25 degrees C. Treatment with carbonylcyanide m-chlorophenylhydrazone, salicylhydroxamic acid + KCN, or arsenite also increased Ca(2+) content of cells. Results are discussed with regard to membrane permeability changes, the cellular control of Ca(2+), and heat treatments used to inhibit softening of fruit during postharvest storage.

Journal Article↗
PubMed1986

Purification of a protein C activator from the venom of the southern copperhead snake (Agkistrodon contortrix contortrix).

A protease has been purified by ion-exchange chromatography from the venom of Agkistrodon contortrix contortrix (Southern copperhead snake) that can activate the vitamin K dependent protein, protein C. The apparent molecular weight of this protease, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was 20,000 under nonreducing conditions. Incubation of this protease with plasma resulted in a prolongation of the clotting time and a time-dependent increase in amidolytic activity. Incubation of the protease with purified protein C resulted in an increase in both amidolytic and anticoagulant activity. The protease had no inhibitory effect on thrombin, factor V, fibrinogen, or factor X. It had slight clotting activity toward fibrinogen. The apparent Km of the protease for protein C was 0.28 microM. Calcium ions were observed to inhibit protein C activation with an apparent Ki of 0.2 mM. Ethylenediaminetetraacetic acid, diisopropyl fluorophosphate, and soybean trypsin inhibitor were observed to inhibit the venom protease. These results suggest that the venom of the Southern copperhead snake contains a protease that is a specific activator of protein C.

Animals↗
PubMed1980

Physical and psychological follow-up on offspring of renal allograft recipients.

In light of concern regarding the effect of pregnancy on renal allograft recipients and the lack of information about their offspring, we studied ten infants born to eight allograft recipients (five female and three male). In all five female recipients, allograft function remained normal throughout pregnancy and the puerperium. Complications of pregnancy were minimal. Four patients were delivered by cesarean section, three for obstetrical reasons and one electively. Three of ten infants were premature births. There were no significant neonatal complications. At time of follow-up study, the offspring were 4 months to 6 8/12 years of age; physical examination was normal on all offspring. Developmental test results ranged from 82 to 131. Parents tended to be overly cautious and sought medical attention at the first sign of illness. Our data indicated no adverse effect on renal allograft function of the female recipients and no physical or developmental abnormalities in the offspring.

Adult↗
PubMed1976

An influenza B epidemic among children in day-care.

An outbreak of influenza virus type B infections occurred in the Frank Porter Graham Day Care Center from February to April, 1974. During the epidemic there were 27 isolations of influenza B virus from 20 children. One half of these were obtained from children who were well at the time of culturing. Attack rates as determined by virus isolation and seroconversion for most age groups approximated the 40% rate for the entire population. Clinical and microbiological data from this study were compared to those obtained in the center during the same three months in 1973. Increases in the incidence of otitis media and temperatures over 38 C were seen during the 1974 study. Intensive continuous microbiological surveillance for bacteria, viruses, and mycoplasmas failed to reveal predominance of any other potential pathogen to account for these clinical findings. The analysis permitted by the nature of this study design in a day-care setting revealed several unexpected findings: high attack rates unrelated to age; common inapparent infections; and frequent association with otitis media.

Child↗
PubMed1974

Pathogenesis of human parainfluenza type 3 virus infection in hamster tracheal organ culture.

Hamster tracheal organ culture was employed as a model for the study of the pathogenesis of human parainfluenza type 3 virus infection. It clearly supported replication of the virus over a 2-week period of time. Infected tracheal explants were examined with light, electron, and immunofluorescence microscopy. They exhibited specific cytopathologic alterations including nuclear swelling and chromatin margination, multinucleated syncytia and binucleated epithelial cells, and fibroblasts and chondrocytes. Focal destruction and denudation of the respiratory epithelium occurred in later stages of infection. Virus was detected in close association with cilia and was observed budding off the unit membrane of epithelial cells.

Animals↗
PubMedDate not supplied

Pediatric Lyme arthritis: clinical spectrum and outcome.

A cohort of children with Lyme arthritis was used to evaluate the clinical and serologic profile of the disease. During a 42-month period (June 1989 to December 1991), 44 patients (13 girls and 31 boys, ages 4-18 years) were included and followed for 6-36 months. Inclusion required the presence of arthritis, as well as positive serology. Thirty-four children with juvenile rheumatoid arthritis or spondyloarthropathy were used as a serologic comparison group. Five different patterns of arthritis were found. Preceding erythema migrans was seen in seven children. Antinuclear antibodies were positive in 30% of the patients. Three treatments were used and selected according to physician preference, patient age, and presence of extraarticular disease: amoxicillin, doxycycline, and ceftriaxone. Articular disease reached complete resolution in all patients within 2-12 weeks. Lyme arthritis in children may mimic other pediatric arthritides. Prognosis for children with clearly defined Lyme arthritis was excellent.

Adolescent↗