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Biomedical subjects

J D Ferguson

Publications and source records attributed to J D Ferguson.

At least 19 recordsLinked to original sources
PubMed2006

Body condition assessment using digital images.

This project assessed the ability to assign a body condition score (BCS) to a dairy cow from digital photographs or videos. Images were taken from the rear of the cow at a 0 to 20 degrees angle relative to the tail head. Four observers assigned a BCS to each of 57 cows at a farm visit (live, farm 1) and later from a photograph (photo). Means +/- standard deviations of BCS by method and observer were as follows: live = 3.25 +/- 0.51, 3.42 +/- 0.49, 3.32 +/- 0.58, 3.13 +/- 0.62; photo = 3.36 +/- 0.52, 3.32 +/- 0.43, 3.44 +/- 0.62, 3.14 +/- 0.6 for observers 1 to 4, respectively. Body condition score means differed across observers for live (observer 2 higher and observer 4 lower, compared with observers 1 and 3) and photo methods (observer 3 lower, compared with observers 1, 2, and 3); however, within observer, the mean live BCS did not differ from the mean photo BCS. Correlation coefficients between BCS assigned live and from photos were 0.84, 0.82, 0.82, and 0.90 for observers 1 to 4, respectively. Subsequently, observer 1 visited 2 farms, assigned a live BCS, and digitally photographed 187 cows (56 and 131 cows from farms 2 and 3, respectively). Observers 2, 3, and 4 assigned a BCS from the photographs. Means +/- standard deviations of BCS by observer (method) were 1 (live) 3.35 +/- 0.55; 2 (photo) 3.33 +/- 0.49; 3 (photo) 3.60 +/- 0.54; and 4 (photo) 3.26 +/- 0.62. The mean BCS for observer 3 was higher and that for observer 4 was lower than for observers 1 and 2. Correlation coefficients between observer 1 and observers 2 through 4 were 0.78, 0.76, and 0.79, respectively. Observer 1 assigned a BCS to 41 cows at a farm visit and 3 wk later assessed the BCS of cows from a video taken at a farm visit by a different individual. Cows were restrained in headlocks at a feed bunk when assessing BCS and for video production. No difference was detected for the mean BCS, for the standard deviation of the mean BCS, or in the distribution of BCS between the live and video assessments. Mean and SD for 17 groups of Holstein cows from 20 farms were used to generate 10,000 random samples of BCS. Groups of 25, 50, 100, and 150 cows were created from the random samples, and estimates of mean BCS were determined by sampling 3 to 80% of the group. Estimates of mean BCS with a sample size of 30% or more from a group of cows fell within the 95% confidence limit of the true mean more than 98% of the time. Digital photographs provide adequate imaging for assessment of BCS. Sampling 30% of a group should be adequate to assess the mean BCS. Video imaging allowed a rapid assessment of BCS but did not permit identification of individual cows.

Animals↗
PubMed2005

Unimolecular rate constants for HX or DX elimination (X = F, Cl) from chemically activated CF3CH2CH2Cl, C2H5CH2Cl, and C2D5CH2Cl: threshold energies for HF and HCl elimination.

Vibrationally activated CF(3)CH(2)CH(2)Cl molecules were prepared with 94 kcal mol(-1) of vibrational energy by the combination of CF(3)CH(2) and CH(2)Cl radicals and with 101 kcal mol(-1) of energy by the combination of CF(3) and CH(2)CH(2)Cl radicals at room temperature. The unimolecular rate constants for elimination of HCl from CF(3)CH(2)CH(2)Cl were 1.2 x 10(7) and 0.24 x 10(7) s(-1) with 101 and 94 kcal mol(-1), respectively. The product branching ratio, k(HCl)/k(HF), was 80 +/- 25. Activated CH(3)CH(2)CH(2)Cl and CD(3)CD(2)CH(2)Cl molecules with 90 kcal mol(-1) of energy were prepared by recombination of C(2)H(5) (or C(2)D(5)) radicals with CH(2)Cl radicals. The unimolecular rate constant for HCl elimination was 8.7 x 10(7) s(-1), and the kinetic isotope effect was 4.0. Unified transition-state models obtained from density-functional theory calculations, with treatment of torsions as hindered internal rotors for the molecules and the transition states, were employed in the calculation of the RRKM rate constants for CF(3)CH(2)CH(2)Cl and CH(3)CH(2)CH(2)Cl. Fitting the calculated rate constants from RRKM theory to the experimental values provided threshold energies, E(0), of 58 and 71 kcal mol(-1) for the elimination of HCl or HF, respectively, from CF(3)CH(2)CH(2)Cl and 54 kcal mol(-1) for HCl elimination from CH(3)CH(2)CH(2)Cl. Using the hindered-rotor model, threshold energies for HF elimination also were reassigned from previously published chemical activation data for CF(3)CH(2)CH(3,) CF(3)CH(2)CF(3), CH(3)CH(2)CH(2)F, CH(3)CHFCH(3), and CH(3)CF(2)CH(3). In an appendix, the method used to assign threshold energies was tested and verified using the combined thermal and chemical activation data for C(2)H(5)Cl, C(2)H(5)F, and CH(3)CF(3).

Journal Article↗
PubMed2003

Phosphorus feeding levels and critical control points on dairy farms.

A viable and cost-effective approach to managing P on dairy farms is to minimize excess P in diets, which in turn leads to less excretion of P in manure without impairing animal performance. A questionnaire survey was conducted, coupled with on-site feed and fecal sample collection and analysis on dairy farms in New York, Pennsylvania, Delaware, Maryland, and Virginia. The purpose was to assess dietary P levels and to identify critical control points pertaining to P feeding management. Survey responses, 612 out of 2500 randomly selected farms, revealed a wide range of dietary P concentrations for lactating cows, from 3.6 to 7.0 g/kg of feed DM. The mean was 4.4 g/kg, which was 34% above the level recommended by the NRC for 27.9 kg milk/d, the mean milk yield in the survey. Higher P concentrations in diets were not associated with higher milk yields (n = 98, R2 = 0.057 for the survey farms; n = 92, R2 = 0.043 for farms selected for on-site sampling). However, higher dietary P led to higher P excretion in feces (n = 75, R2 = 0.429), with much of the increased fecal P being water soluble. Phosphorus concentrations in diet samples matched closely with P concentrations in formulated rations, with 67% of the feed samples deviating <10% from the formulations. On 84% of the survey farms, ration formulation was provided by professionals rather than producers themselves. Most producers were feeding more P than cows needed because it was recommended in the rations by these consultants. In conclusion, P fed to lactating cows averaged 34% above NRC recommendations; to reduce excess dietary P, ration formulation is the critical control point.

Animal Feed↗
PubMed2001

An assessment of ammonia emissions from dairy facilities in Pennsylvania.

A survey of 715 Holstein dairy farms in Pennsylvania was used to construct demographics for the average Holstein dairy farm. The average Holstein dairy farm was composed of 69 lactating cows; 11 nonlactating, pregnant cows; 44 heifers; and 18 calves. Milk production averaged 27.3 kg (60.0 lb). Crop area averaged 73.6 ha. Milk production, crop area and type, average county yields, and herd animal groups were used to construct a typical feeding program for these farms. Typical rations were constructed for six feeding groups (three milk production groups, one nonlactating group, two heifer groups) to meet milk production, pregnancy, and growth requirements. Rations were constructed based on three forage qualities (excellent, average, and poor) typically observed on Pennsylvania dairy farms. Data for animal description (milk production, body weight, growth, and pregnancy status) and ration components and amounts consumed for each animal group were input into the excretion model of the Dairy Nutrient Planner computer program (DNP). Excretion of fecal N and dry matter (DM), urinary N, and total P and K were produced for each animal group and used to assess potential volatile losses of N. Work at the Marshak Dairy, New Bolton Center, indicates the majority of urinary N is rapidly lost as ammonia from dairy facilities. Based on this observation, the losses of N as ammonia were estimated to be 4.63, 4.62, and 4.28 tonne/year for the farm with excellent, average, and poor quality forages, respectively. Volatile losses of N may be reduced most by controlling levels of urea in urine. Urinary N may be reduced through dietary manipulation of protein and carbohydrate sources. Conversion of urea to ammonia may be reduced by altering the pH of barn floors and gutters. Entrapment of ammonia may be accomplished by acidification of manure slurry. Atmospheric ammonia contributes to acid rain, eutrophication of estuaries and lakes, and particulate air pollution. Reduction of ammonia emissions from dairy barns can significantly reduce atmospheric pollution and improve air and water quality.

Air Pollution↗
PubMed2001

A simplified approach to temporary DDD pacing using a single lead, balloon-tipped catheter with overlapping biphasic impulse stimulation.

Temporary DDD pacing offers significant hemodynamic benefits in emergency management of bradyarrhythmias but is underused because of its complexity (two leads) and unreliability. Single lead VDD pacing with atrial sensing via a floating dipole is feasible, but atrial pacing is limited by high thresholds and phrenic nerve stimulation (PNS). Overlapping biphasic impulse (OLBI) stimulation may avoid these problems. The authors designed a single lead balloon-tipped catheter for temporary transvenous DDD pacing, incorporating noncontact atrial dipoles for OLBI stimulation. This catheter was deployed using fluoroscopic guidance in 74 patients (43 men, 31 women) with mean age 56.9 +/- 17.0 years. Pacing parameters were assessed at implantation and follow-up. The median procedural time was 6.6 (range 1.2-25.0) minutes and fluoroscopy time 1.9 (range 0.2-7.8) minutes. Stable VDD pacing was achieved in all cases. Atrial capture was achieved in 73 of 74 cases with both modes at maximum output but was restricted by PNS at outputs below atrial capture threshold in 3 of 74 cases with OLBI and 10 of 74 cases with standard bipolar mode (P = 0.04). At outputs > or = 1.0 V above atrial threshold, reliable DDD pacing without PNS was achieved and maintained in 67 (91%) of 74 patients in OLBI compared to 53 (72%) of 74 patients in bipolar mode (P = 0.003). Pacing parameters were stable during follow-up (median 53 hours, range 6-168 hours). In conclusion, the single lead catheter with OLBI stimulation allows temporary VDD and DDD pacing without PNS to be achieved in > 90% of patients. This rapid and convenient approach should facilitate DDD pacing in emergency settings.

Cardiac Pacing, Artificial↗
PubMed2001

Ultrasound guided percutaneous thrombin injection of iatrogenic femoral artery pseudoaneurysms after coronary angiography and intervention.

Ultrasound guided percutaneous thrombin injection has recently been described for the treatment of iatrogenic femoral pseudoaneurysms. Patient selection and technical aspects of this technique are still evolving and safety data, particularly after coronary intervention, remains limited. The percutaneous thrombin injection of femoral artery pseudoaneurysms in 13 consecutive patients, most of whom were receiving antiplatelet/anticoagulant treatment (aspirin 11, heparin 4, clopidogrel 6), is reported. Thrombin (1000 U/ml) was injected over several seconds until Doppler colour flow within the cavity ceased. The median dose of thrombin injected was 800 U (range 200-1000 U) and the treatment was successful in all cases without complication. In one case, thrombus was visualised within the arterial lumen immediately after thrombin injection, but this dissolved spontaneously within five minutes without evidence of embolisation. In contrast to ultrasound guided compression, percutaneous thrombin injection of femoral pseudoaneurysms is a rapid, well tolerated, and successful technique even in patients receiving antiplatelet/anticoagulant treatment.

Adult↗
PubMed2001

A survey of dairy farming in Pennsylvania: nutrient management practices and implications.

A survey was conducted to collect information on nutrient management practices on dairy farms in south-central Pennsylvania. Of the 994 responding farms, the average farm consisted of 64 lactating cows, 10 dry cows, 41 heifers, and 17 calves with 69.7 ha of tillable land. Manure from lactating cows was mainly collected on a daily basis (84% of the farms) and stored as slurry or liquid (73%), while dry cow and heifer manure was collected weekly or less frequently (69 and 85% of the farms) and stored as solid stack or bedded pack (67 and 82%). Manure utilization featured consistent use of on-farm spreading, with limited incorporation, to corn or small grain fields before planting. Spreading on perennial forages or pasture was also common. Irrigation or injection of manure occurred on less than 5% of the farms. Only 20% of the farms reported manure nutrient testing, contrasting to over 90% for soil testing. Farm advisors and their services can be of vital importance in helping producers make conscientious management decisions for enhanced nutrient utilization. For example, ration balancing involved the services of feed and mineral sales representatives (85% of the farms), independent consultants (12%), and veterinarians (5%). Manure nutrient crediting to determine manure application rates was made by fertilizer dealers (40%), crop advisors and independent consultants (31%), and others. Nutrient management strategies and efforts must address the specific needs of farms with different animal densities and nutrient balances in order to be effective and applicable on the majority of farms.

Agriculture↗
PubMed2000

A method to analyze production responses in dairy herds.

Milk production was simulated in a 50-cow herd averaging 8182 kg of 305-d milk with a standard deviation of 1364 kg. Herd demographics were 35% first lactation, 20% second lactation, and 45% third or greater lactation cows. A lactation model was developed with the Wood's equation (Milk/d = A*DIM*e(-c*dim)) to which random variation was added to be consistent with a coefficient of variation of 10% for daily milk production. Five sequential sampling periods, 30 d apart, were randomly selected for the experiment. For each of these sampling periods data were simulated for cow, lactation number, milk, and days in milk (DIM). To the third sampling period, a known input was pulsed into each cow record to simulate a change in milk production. Inputs and number of herds simulated were -1.140 kg and 15 herds, 0.909 kg and 30 herds, -0.455 kg and 20 herds, 0 kg and 65 herds, 0.455 kg and 21 herds, -0.909 kg and 47 herds, 1.140 kg and 20 herds, and 2.270 kg and 15 herds. Regression by cow was used to estimate milk production change for the known inputs: Milk(ijk) = Intercept + beta(i)*DIM(ij) + TRT(ik) + epsilon(ijk). Parameter estimates for each cow were submitted to analysis of variance with herd as a class variable. The least square mean of TRT (dummy variable for known input of milk volume change) for herd was tested for difference from zero based on a "t" statistic. Herd responses were classed as negative, not different from zero, and greater than zero based on P < 0.10. Herd responses were categorized based on the known input to assess the ability of the method to detect a change in production. The mean estimate of TRT from the regression analysis was used to assess the ability of the method to estimate the magnitude of the known input. The regression method was able to detect changes in production greater than 0.455 kg, but is more useful when changes of 0.9 kg or greater are shown. Adjustment for days postcalving on first test day is necessary to correct for the bias in linear regression to estimate response across the curvilinear milk production function.

Animals↗
PubMed2000

Effects of inclusion of a blended protein product in 35 dairy herds in five regions of the country.

We assessed the change in milk production in 35 dairy herds located in eight states when a blended animal-marine protein product was included in diets at 2 (22 herds) to 4% (13 herds) of dry matter. Average 305-d production across herds was 8844.1 kg (SEM = 153.7 kg) of milk, with a range of 6876.9 to 11,293.2 kg. The mean days in milk for all herds at initiation of the trial was 118.8 d (SEM = 3.7, herd mean range of 68.8 to 160.0 d), and the average daily milk production was 32.6 kg (SEM = 0.6 kg, range 24.2 to 39.6 kg). The animal-marine protein blend was included in herd rations for 30 d, beginning immediately after a DHI herd test month and ending immediately after the next DHI sample test. Cow milk records were collected for 1 to 2 mo before the protein blend was included and for 2 to 3 mo after the protein was removed. Sample days were assigned a dummy variable to indicate months off or on the animal-marine protein blend. A total of 33,190 milk records from 7135 cows were analyzed. The numbers within herd ranged from 35 to 2012 cows. Of the 35 herds, 19 were classed as having increased milk yield, 12 herds as having no change, and 4 herds as having decreased milk yield when the animal-marine protein blend was included in the diet. The population mean for change in milk yield with the inclusion of the animal-marine protein blend was 1.24 kg/d of milk (SEM = 0.05 kg). There was no significant effect of parity on mean response. Milk protein content was not influenced by animal-marine protein blend inclusion. Fat content was lower for the month on which the animal-marine protein blend was fed (3.51%) compared with the month prior (3.63%) and the month after (3.70%), respectively (SEM = 0.032). Stage of lactation influenced the method for calculating the production response and the actual response to the animal-marine protein blend.

Animal Feed↗
PubMed1997

Milk production and reproductive performance in dairy cows given bovine respiratory syncytial virus vaccine prior to parturition.

OBJECTIVE: To assess the effect of vaccination against bovine respiratory syncytial virus on milk production, reproductive performance, and health in lactating dairy cows. DESIGN: Prospective randomized block design. ANIMALS: 385 Holstein dairy cows and heifers. PROCEDURE: Cows were grouped by lactation number, season of calving, and previous mature equivalent 305-day milk production (where appropriate). Prior to parturition, cows and heifers were randomly assigned to be vaccinated i.m. against infectious bovine rhinotracheitis, bovine viral diarrhea, and parainfluenza 3 viruses by use of a three-way vaccine, or to be vaccinated against those viruses as well as bovine respiratory syncytial virus, using a four-way vaccine. Milk production was measured daily through 305 days of lactation. Reproductive and medical records were reviewed to obtain insemination dates and record medical problems of cows in each vaccine treatment group. RESULTS: Compared with the three-way vaccine, administration of the four-way vaccine was associated with higher milk production (1.39 kg [3.06 lb] more milk/d) in first-parity cows during the first 21 weeks of lactation. Vaccination did not have any effect on milk production after the first 21 weeks of lactation in cows of any parity. Conception rates at first insemination were higher for four-way vaccinated first-parity cows than for three-way vaccinated first-parity cows (54.6 vs 32.7%). Compared with second-parity cows that received the three-way vaccine, first insemination conception rate was improved for second-parity cows vaccinated with the four-way vaccine (28.9 vs 47.8%, respectively). In cows of third or greater parity, first insemination conception rate was not different between the 2 vaccine treatment groups. CLINICAL IMPLICATIONS: Vaccination of heifers against bovine respiratory syncytial virus prior to partrition may increase milk production and first insemination conception rates.

Animals↗
PubMed1996

Responses of dairy cows supplemented with somatotropin during weeks 5 through 43 of lactation.

Beginning at wk 5 of lactation, 136 cows (34 per treatment) were supplemented daily for 38 wk with 0, 10.3, 20.6, or 41.2 mg of recombinantly derived bST monomer. Cows were obtained from University of Kentucky, University of Minnesota, University of Pennsylvania, and The Ohio State University. Nine cows (4 at 0 mg/d, 1 at 10.3 mg/d, 1 at 20.6 mg/d, and 3 at 41.2 mg/d) did not complete the experiment because of health problems. Data from these cows were included in the reproduction and health databases but not in the production database. Cows supplemented with bST produced more milk, consumed more feed, had lower rates of BW gain, and had improved efficiencies of milk production (conversion of feed and NEL to milk). Additional increases in productivity were modest at 20.6 and 41.2 mg/d versus productivity at 10.3 mg/d of bST. Concentrations of fat, protein, and TS in milk were unaffected. At 10.3 mg/d, bST did not adversely affect reproduction or health.

Animal Nutritional Physiological Phenomena↗