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Biomedical subjects

J Cui

Publications and source records attributed to J Cui.

At least 163 records · Page 9Linked to original sources
PubMed1997

[LH-CG receptor protein expression in epithelial ovarian cancer].

OBJECTIVE: To evaluate the relationship between (LH-CG) receptor expression and prognosis in epithelial ovarian cancer. METHODS: The relative quantity of LH-CG receptor protein of epithelial ovarian cancer tissuse was detected with semiquantitative western immunobloting in 40 cases. The LH-CG receptor protein was located with immunohistochemistry. The LH-CG receptor was termed high expression if its concentration was more than and/or equal to the median, and low expression if its concentration was less than the median. 36 of the 40 cases were followed up at various intervals, the longest follow up period was 56 months. There were 16 cases with high LH-CG expression and 20 cases with low LH-CG receptor expression in which 9 cases died. RESULTS: The positive rate of LH-CG receptor protein expression was 72.5% (29/40). The level of LH-CG receptor protein expression in patients with stages I and II was higher than that in patients with stages III and IV, but it is not significant (P > 0.05). The LH-CG receptor concentration in the well-differentiated cancer group was twice as much as that in the poorly-differentiated cancer group. The difference between the well-differentiated group and the poorly-differentiated group was significant (P < 0.05). The 1-year and 3-year survival rates were 83.94% and 67.15% respectively in the high LH-CG receptor expression group. Both of the 1-year and 3-year survival rates were 33. 34% in the low LH-CG receptor expression group. The survival rates of the high expression group was significantly higher than those of the low expression group (P < 0.05). LH-CG receptor expression did not correlate with age, lymph node metastases, the size of residual tumor and CA125. CONCLUSION: The prognosis of patients with high LH-CG receptor expression is better than that of those with low expression.

Adult↗
PubMed1997

[Using lyophilization to the preparation of drug paper disks for antimicrobial susceptibility test].

It was found that the paper disks used in the antimicrobial susceptibility test could be prepared by natural drying at 37 degrees C, but the drug distribution between these disks is not so enen as determined by antimicrobial inhibitory test, thus it was difficult to fit the needs of the national standards. Alternatively, we applied the lyophilization technique to the disks preparation and 12 batches, of different antibiotic disks were made out and found that the difference between disks is reduced, and all are meet to the national requirement. It is supposed that the capillary action may play a role in the variation of drug distribution in the natural drying process, and the lyophilization process avoided the capillary action, hence the more even distributed antibiotic disks are produced. The preparation of lyophilized disks is a simple and practical method that could be used in the antimicrobial susceptibility tests in hospital.

Freeze Drying↗
PubMed1996

Fatal haemorrhage and incomplete block to embryogenesis in mice lacking coagulation factor V.

Coagulation factor V is a critical cofactor for the activation of prothrombin to thrombin, the penultimate step in the generation of a fibrin blood clot. Genetic deficiency of factor V results in a congenital bleeding disorder (parahaemophilia), whereas inheritance of a mutation rendering factor V resistant to inactivation is an important risk factor for thrombosis. We report here that approximately half of homozygous embryos deficient in factor V (Fv-/-), which have been generated by gene targeting, die at embryonic day (E) 9-10, possibly as a result of an abnormality in the yolk-sac vasculature. The remaining Fv-/- mice progress normally to term, but die from massive haemorrhage within 2 hours of birth. Considered together with the milder phenotypes generally associated with deficiencies of other clotting factors, our findings demonstrate the primary role of the common coagulation pathway and the absolute requirement for functional factor V for prothrombinase activity. They also provide direct evidence for the existence of other critical haemostatic functions for thrombin in addition to fibrin clot formation, and identify a previously unrecognized role for the coagulation system in early mammalian development.

Animals↗
PubMed1996

Essential requirement of an invariant V alpha 14 T cell antigen receptor expression in the development of natural killer T cells.

NK1.1+ T [natural killer (NK) T] cells express an invariant T cell antigen receptor alpha chain (TCR alpha) encoded by V alpha 14 and J alpha 281 segments in association with a limited number of V betas, predominantly V beta 8.2. Expression of the invariant V alpha 14/J alpha 281, but not V alpha 1, TCR in transgenic mice lacking endogenous TCR alpha expression blocks the development of conventional T alpha beta cells and leads to the preferential development of V alpha 14 NK T cells, suggesting a prerequisite role of invariant V alpha 14 TCR in NK T cell development. In V beta 8.2 but not B beta 3 transgenic mice, two NK T cells with different CD3 epsilon expressions, CD3 epsilon(dim) and CD3 epsilon(high), can be identified. CD3 epsilon(high) NK T cells express surface V alpha 14/V beta 8 TCR, indicating a mature cell type, whereas CD3 epsilon(dim) NK T cells express V beta 8 without V alpha 14 TCR and no significant CD3 epsilon expression (CD3 epsilon(dim)) on the cell surface. However, the latter are positive for recombination activating gene (RAG-1 and RAG-2) mRNA, which are only expressed in the precursor or immature T cell lineage, and also possess CD3 epsilon mRNA in their cytoplasm, suggesting that CD3 epsilon(dim) NK T cells are the precursor of V alpha 14 NK T cells.

Animals↗
PubMed1996

Lack of plasminogen activator inhibitor-1 effect in a transgenic mouse model of metastatic melanoma.

Tumor cell invasion and metastasis is a complex, multistep process that is postulated to require degradation of extracellular matrix at several steps. Urokinase-type plasminogen activator (uPA) is expressed on the cell surface of B16 murine melanoma cells and is thought to contribute to the pericellular proteolysis necessary for tumor cell migration. In vitro modification of B16 melanoma cell surface uPA activity has been shown to alter the invasive and metastatic potential of these murine melanoma cells in vivo. Plasminogen activator inhibitor-1 (PAI-1), a rapid inhibitor of both uPA and tissue-type plasminogen activator (tPA) is the major physiologic regulator of plasminogen activator activity. To test the role of host PAI-1 in the invasive and metastatic capacity of B16 melanoma cells we analyzed local tumor growth and pulmonary metastasis in transgenic mice engineered to overexpress murine PAI-1 in multiple tissues including lung, and in mice completely deficient in PAI-1. No significant difference in the number of pulmonary metastases was observed after intravenous inoculation of tumor cells into PAI-1-overexpressing and PAI-1-deficient mice when compared with wild-type controls. Similarly, in a spontaneous metastasis model, PAI-1-overexpressing and PAI-1-deficient mice demonstrated no difference in primary tumor size or overall survival. These data demonstrate that wide variations of host PAI-1 expression, from complete absence to marked overexpression, does not significantly influence the metastatic potential of B16 melanoma cells in a murine model.

Animals↗
PubMed1996

V alpha 14+ NK T cells: a novel lymphoid cell lineage with regulatory function.

A novel lymphoid lineage, NK T cells, was recently found. The NK T cells are the major population in the periphery comprising 5% of splenic T cells and 40% of bone marrow T cells. They express a unique TCR composed of invariant V alpha 14J alpha 281 and V beta 8.2 together with NK receptor (NKRPI). Surprisingly, the invariant V alpha 14+ TCR is exclusively expressed on NK T cells but not on conventional T cells. As the selective decrease in V alpha 14+ NK T cell population in the periphery is tightly correlated with autoimmune disease development, V alpha 14+ NK T cells control development of autoimmune diseases. We also found that V alpha 14 TCR gene rearrangement and transcripts were detected at an early embryogenesis (d9.5) before the thymus formation. Therefore NK T cells are in the distinct category from conventional T cells. The target of NK T cells is found to be CD1 (class 1b, monomorphic class I MHC-like molecule) present on bone marrow-derived cells and is killed by Fas-FasL interaction or perforin-mediated mechanisms. These results indicate that NK T cells consist of an immunoregulatory system different from defense system in terms of homogeneous repertoire, extrathymic development in early stage of gestation, and their regulatory functional role.

Animals↗
PubMed1996

Sympathetic outflow response to muscle during vestibular stimulation in humans.

To observe the effects of caloric vestibular stimulation on muscle sympathetic nerve activity (MSNA) in humans, 14 healthy volunteers were monitored in a supine position by electrocardiogram (ECG), blood pressure (BP), electro-oculogram (EOG). MSNA was monitored by a double recording technique of microneurography from the bilateral tibial nerves. Caloric vestibular stimulation was loaded by injecting 50 ml 44 degrees C warm water and 50 ml 10 degrees C cold water alternately into the external meatus for 1 min. Nystagmus was evoked in all cases by cold stimulation and in some cases by hot stimulation. The nystagmus evoked by cold stimulation was more intense than that by hot stimulation. MSNA was enhanced by either cold or hot stimulation; however, the enhancement mode differed between cold and hot stimulation. Cold stimulation evoked two peaks of MSNA while hot stimulation elicited only one peak. The first peak (404.5 +/- 115.4% with control value set at 100%, mean +/- SE) was estimated to be caused by cold stimulation on the skin of the external meatus while the second peak (379.2 +/- 65.3%) seemed to be the result of vestibular stimulation. With hot stimulation, the response peak of MSNA was 243.3 +/- 28.1%. In general, MSNA was enhanced after vestibular stimulation with MSNA increases was proportional to the stimulated level of the vestibular system.

Adult↗
PubMed1996

Exercise echocardiography: feasibility and value for detection of coronary artery disease.

OBJECTIVE: To evaluate the feasibility and accuracy of exercise echocardiography (Ex-Echo) for the diagnosis of coronary artery disease (CAD) based on coronary angiography (CA). PATIENTS AND METHODS: Forty-seven patients were found to have CAD and examined by upright exercise electrocardiography (Ex-ECG) on a treadmill within two weeks of CA. Before and immediately after exercise, the patients lay on a bed beside the treadmill in the left lateral position and parasternal long and short axis and apical two and four chamber views of the heart were acquired. Pre- and post-echocardiograms were analysed in a side-by-side multiple screen format on the imaging view and hypodynamic wall motion of the left ventricle after exercise was defined as Ex-Echo positive. The sensitivity, specificity and predictive accuracy of Ex-Echo and Ex-ECG were calculated on the basis of CA data. RESULTS: Satisfactory echocardiograms were recorded in 46 patients after exercise. The success rate was 97.8%. Compared with Ex-ECG, Ex-Echo was more sensitive (87.5% vs 62.5%, P < 0.05), specific (92.8% vs 60.0%, P < 0.05) and accurate (89.4% vs 61.7%, P < 0.01). The concord of determining the number of diseased vessels between coronary angiography and Ex-Echo was 90.9% in single vessel disease and 45.0% in multiple vessel disease. Wall motion scoring index, however, was higher in multiple than in single vessel CAD. CONCLUSIONS: Ex-Echo test is feasible and accurate in detecting CAD and wall motion scoring index is probably useful in distinguishing multiple from single vessel CAD.

Coronary Disease↗
PubMed1996

[Deletion and down-regulation of mts1/p16 gene in human gastric cancer].

Deletion of mts1/p16 gene was frequently detected in many human tumor cell lines. However, whether alteration of the p16 gene was involved in human gastric carcinogenesis, it is not clear. In order to determine the incidence and correlation of the p16 gene deletion with human gastric cancer, we performed analyses of PCR, Southern and Northern blotting on 85 fresh tumor specimens and 5 tumor cell lines from gastric cancer patients. Homozygous deletion was observed in 1 cell line and down-regulation of expression was observed in 3. High rate of gene deletion was detected by PCR in 14 of 85 fresh tumor tissues. Gene deletion was confirmed by Southern blot analysis with p16 cDNA probe in 6 out of 26 tumor specimens examined.

Cyclin-Dependent Kinase Inhibitor p16↗
PubMed1996

[The epidemiological and clinical features of 208 patients with trichinosis].

In order to know the epidemiological and clinical features of trichinosis, the data of 208 patients with trichinosis from 1992 to 1994 were analysed. The results showed that these patients came from 11 districts, and acquired the infection mainly by tasting the raw pork filling for dumplings or ingesting instant-boiled pork or mutton. The incidence of trichinosis is high in winter. Young and middle-aged workers and cadres constituted the majority of the patients and the infection was more common in the males than in females. The main clinical manifestations of trichinosis were prolonged fever, general myalgia, muscle weekness and eosinophilia. Most of the patients had no gastrointestinal symptoms and skin eruption. Eyelid edema was only seen in the early stage. Serological tests were significant value in the diagnosis of trichinosis. The key measures to prevent trichinosis were that meat inspection should be strictly carried out and bad eating habit changed.

Adolescent↗
PubMed1996

[Interventional treatment for partial stenosis or occlusion type of Budd-Chiari syndrome].

It is difficult to deal with interventional management of partial stenosis or occlusion type of Budd-chiari syndrome and manage the hepatic veins associated with inferior vena cave occlusion. Puncture, PTA and vascular stent plant were used to treat 12 patients with partial stenosis or occlusion of Budd-chiari syndrome. The procedures were successful. Either the symptoms or signs disappeared or relieved after operation. No severe side effects occurred. Follow-up for 1.5 through 26 months (average 8.5 months) revealed that the early or middle results were gratifying. There may be danger during operation, but perfect skills and adequate clinical anatomic knowledge help avoid severe side effects.

Adult↗
PubMed1995

Improved survival of patients with melanoma with an antibody response to immunization to a polyvalent melanoma vaccine.

BACKGROUND: Melanoma vaccine treatment appears to slow the progression of melanoma in some patients, particularly in patients in whom it stimulates cellular antimelanoma immune responses. The relationship of vaccine-induced antibody responses to clinical outcome is less clear. The purpose of this study was to investigate the clinical relevance of antibody responses to melanoma vaccine immunization. METHODS: Eighty-two evaluable patients with surgically resected American Joint Committee on Cancer Stage III malignant melanoma were immunized to a partially purified, polyvalent, melanoma antigen vaccine. Antimelanoma antibodies were measured by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis before vaccine treatment and 1 week after the fourth immunization. RESULTS: Vaccine treatment induced or augmented antibody responses to melanoma in 32 (39%) of the patients. The antibodies were directed to one or more antigens of 38-43, 75, 110, 150 and/or 210 kDs, which previously have been shown to be expressed preferentially in cultured human melanoma cells. The median disease free survival of patients with a vaccine-induced antibody response to one or more of these antigens was 5.4 years compared with 1.4 years for nonresponders (P = 0.06), and 5-year overall survival was 71% compared with 44%, respectively (P = < 0.01). As determined by Cox multivariate analysis, the difference in overall survival was independent of disease severity or of immunologic competence as evaluated by ability to be sensitized to dinitrochlorobenzene. The difference in survival between antibody responders and nonresponders improved with time. CONCLUSIONS: The antibody response to vaccine treatment is an immune marker of vaccine activity that appears to be predictive of a later reduction in the recurrence of melanoma and is unrelated to the vaccine's ability to induce cellular immune responses. This finding suggests that vaccine treatment may be effective in slowing the progression of melanoma in some patients and that the protective effect is mediated partly by vaccine-induced antimelanoma antibodies.

Antibody Formation↗
PubMed1995

Characterization of vitiligo antigens.

Patients with vitiligo have circulating antibodies directed in part to pigment cell antigens with MWs of approximately 90, 75, and 40-45 kDs. These antigens are denominated VIT 90, VIT 75, and VIT 40, respectively. To further characterize these "vitiligo" antigens, we examined their relation to antigens defined by a panel of 25 monoclonal antibodies (moab) to pigment cell antigens. We found by immunoprecipitation and SDS-PAGE analysis of 125I labelled, detergent soluble, human melanocyte macromolecules, that 24 (83%) of 29 patients with vitiligo had antibodies to one or more vitiligo antigens vs. 2 (7%) of 28 control individuals. Seventeen of the 25 moabs did not react with any labelled antigen in the same lysate. Of the remaining eight moabs, only four precipitated an antigen that co-migrated with one of the vitiligo antigens. Moab TA99, HMSA-5, and TMH-1 (all directed to the 75 kD tyrosinase-related protein [TRP1]) co-migrated with VIT 75. Moab W6/32 (directed to class I HLA antigen) co-migrated with VIT 40. Immunodepletion studies with vitiligo antibodies selectively depleted the antigen defined by W6/32 but not the antigen defined by TA99 and HMSA-5, indicating that VIT 75 was not the 75 kD tyrosinase-related protein. The vitiligo antigens were easily labelled by the lactoperoxidase technique but poorly labelled with 35S-methionine, suggesting they are expressed on the cell surface. These studies indicate that VIT 90 and VIT 75 differ from antigens defined by currently available moabs to pigment cell antigens. VIT 40 appears to share a cross-reactive epitope, or be tightly bound to, class I HLA antigen.

Antibodies, Monoclonal↗
PubMed1995

Antimelanoma antibodies in swine with spontaneously regressing melanoma.

Sinclair swine provide a unique model for studying mechanisms of tumor regression because they are born with melanomas that spontaneously regress approximately 10 weeks after birth. To examine whether an antitumor immune response is present in these animals, and, if so, to study its relation to tumor regression, 38 sera specimens collected at different times from 13 swine born with melanomas were tested for melanoma antibodies by immunoprecipitation and SDS-PAGE analysis of 125I labelled swine melanoma macromolecules. Antibodies to melanoma were present in 13 (100%) of the swine versus 1 of 3 control swine. The antibodies were directed to antigens of approximately 45, 68-75, or 100 kDa. These antigens were also expressed on human melanomas and normal melanocytes but on only one of five unrelated tumors. The incidence and level of these antibodies increased with time. Antibodies to the 45, 68-75, and 100 kDa antigens were present in 36%, 55%, and 9%, respectively, of sera collected prior to 7 weeks of age, but in 80%, 100%, and 37% of sera collected between 7 and 20 weeks (P < 0.05). The rise in melanoma antibodies usually preceded or appeared together with tumor regression and loss of pigmentation. These findings indicate that Sinclair swine with melanomas have antibodies to antigens preferentially expressed on pigment cells, and support the hypothesis that the regression phenomenon and the vitiligo-like skin depigmentation result from immune responses to common antigens shared by normal and malignant swine pigment cells.

Aging↗
PubMed1995

Melanoma and vitiligo are associated with antibody responses to similar antigens on pigment cells.

BACKGROUND AND DESIGN: Several clinical observations suggest that there is a link between vitiligo and melanoma. We examined whether an immune response to similar antigens on pigment cells could account for this association. We tested 30 patients with melanoma, 29 patients with vitiligo, and 28 patients with unrelated conditions for antibodies to human melanocyte antigens using an immunoprecipitation sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis assay. RESULTS: Antibodies to melanocytes were present in 24 (80%) patients from the melanoma group, 24 (83%) patients from the vitiligo group, and in two (7%) patients from the control group. The antibodies in patients with melanoma or vitiligo were directed to similar antigens with molecular weights of approximately 40 to 45, 75, and 90 kd. The frequency of antibody responses to each of these antigens was similar in both diseases. By sequential immunodepletion, the antigens defined by antibodies in both diseases were similar. These antigens were also expressed on melanoma cells. CONCLUSIONS: Most patients with melanoma or with vitiligo develop antibodies to similar antigens that are present both on melanocytes and on melanoma cells. These findings support the hypothesis that the clinical link between the two diseases results from immune responses to antigens shared by normal and malignant pigment cells.

Antibodies↗
PubMed1995

Presence of antilamin antibodies in sera of patients with systemic lupus erythematosus.

In this study, we characterized specifically-stained sera from patients with systemic lupus erythematosus (SLE) which had been shown to display the homogeneous or peripheral region of nuclei by indirect immunofluorescence (IIF). By western blotting, we demonstrated that in some cases there was a correlation between the peripheral or homogenous. IIF staining of nuclei by sera from patients with SLE and the presence of autoantibodies to lamins. Here we first report the presence of 2.2% anti-lamin autoantibodies in the sera among the 174 patients with SLE in China.

Autoantibodies↗