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J Crocker

Publications and source records attributed to J Crocker.

At least 145 records · Page 8Linked to original sources
PubMed1988

Salivary gland tumours studied by means of the AgNOR technique.

Difficulty is sometimes encountered in distinguishing between pleomorphic adenoma, adenoid cystic carcinoma and adenocarcinoma, especially in small biopsies from salivary glands. The argyrophil (AgNOR) staining technique for nucleolar organizer regions (NORs) has been applied to a series of benign and malignant salivary gland tumours. We studied 35 salivary gland tumours, 13 benign and 22 malignant. In all specimens clearly defined silver-stained intranuclear AgNOR dots were visible. The differences between the numbers of AgNORs in the benign and malignant groups, notably pleomorphic adenomas, adenoid cystic carcinoma and adenocarcinoma, were highly significant. In view of this difference we propose that the AgNOR staining technique is of diagnostic help in distinguishing between these salivary gland tumours.

Histocytochemistry↗
PubMed1988

Intraepithelial neoplasia, human papilloma virus infection and argyrophilic nucleoprotein in cervical epithelium.

A silver colloid technique was applied to 50 colposcopic biopsies of cervix. These comprised nine cases of wart virus infection of the cervix, 11 cases of cervical intraepithelial neoplasia (CIN) I, nine cases of CIN II, eight cases of CIN III, seven normal biopsies and six cases showing only incomplete squamous metaplasia. The mean numbers of argyrophilic nucleolar organizer regions (AgNORS) increased from CIN I to CIN III. Statistically significant differences for AgNORs were found in comparisons between CIN III, normal basal cells, human papilloma virus-infected basal cells and incomplete squamous metaplasia, and in comparisons between normal basal cells and human papilloma virus-infected basal cells. CIN III could be distinguished from incomplete squamous metaplasia and from basal cells and from human papilloma virus-infected basal cells. The latter could be distinguished from normal basal cells on the basis of their AgNORs. It is suggested that this simple technique is diagnostically useful and has considerable clinicopathological potential in cervical pathology and cytology.

Epithelium↗
PubMed1988

Nucleolar organiser regions in fibrous proliferations of childhood and infantile fibrosarcoma.

A silver colloid technique to identify nucleolar organiser region associated protein (AgNOR) was applied to 16 fibrous proliferations of childhood and six low grade fibrosarcomas. The fibrous proliferations comprised five cases of infantile digital fibromatosis, seven of infantile desmoid type fibromatosis, and four of infantile myofibromatosis. The AgNORs were visualised as dots within the nuclei of the cells, and on the basis of their relative mean numbers of AgNORs fibrous proliferations of childhood could be easily differentiated from low grade infantile fibrosarcoma. The differences observed were significant (0.01 greater than p greater than 0.001). This technique, previously the province of the cytogeneticist, may be of use to the pathologist in differentiating infantile fibrous proliferations.

Child↗
PubMed1988

Nucleolar organiser regions in normal, cirrhotic, and carcinomatous livers.

A series of 54 liver biopsy specimens was studied by means of the argyrophil (AgNOR) technique for nucleolar organiser region (NOR)-associated proteins. These included normal livers and livers affected by chronic active hepatitis, cirrhosis, hepatocellular carcinoma and adenoma. Four of the cases of cirrhosis showed liver cell dysplasia. The mean numbers of NOR sites in normal, cirrhotic, and carcinomatous livers were significantly different: adenoma had similar mean counts to those in chronic active hepatitis (CAH). There was no overlap between the ranges of NOR counts in normal, cirrhotic, and malignant liver specimens. Where cirrhosis and hepatocellular carcinoma were present in the same specimen, the AgNOR counts were higher in the carcinomatous than cirrhotic areas. To investigate the prospective value of the method a further seven biopsy specimens were studied; in these it had not been possible to decide on a diagnosis between normality and cirrhosis or cirrhosis and hepatocellular carcinoma. In all seven specimens a repeat biopsy or necropsy gave results as predicted by AgNOR staining. It is therefore proposed that quantitation of staining for NOR-associated proteins is a diagnostically useful method in liver disease.

Adenoma↗
PubMed1988

HMFG-2 as a prognostic indicator in superficial bladder cancer.

A series of transitional cell carcinomas and mucosal biopsy specimens of bladder were stained immunohistochemically with the monoclonal antibody HMFG-2. Staining characteristics ranged from luminal staining in well differentiated, superficial lesions to staining of all cells in invasive carcinomas. Invasive tumour nests also stained strongly with the antibody. There was good correlation between the staining pattern and histological assessment of both tumours and mucosal biopsy specimens. Correlation with clinical course showed that it was also a good predictor of subsequent disease progression of superficial tumours. It is suggested that HMFG-2 staining is a sensitive indictor of malignant transformation and that it may have a role as an adjunct to conventional histological examination in the assessment of urothelial carcinomas.

Antibodies, Monoclonal↗
PubMed1988

Expression of CEA, CA125, CA19-9 and human milk fat globule membrane antigen in ovarian tumours.

The expression of five different antigens in ovarian tumours was studied by means of an immunohistochemical test with anti-CEA, HMFG1 and HMFG2, NS19-9 and OC125 antibodies. Considerable variation was noted not only between different histological types and between tumours of one type but also between areas in a single tumour. HMFG1 and HMFG2 were the most reactive of all the antibodies; NS19-9 and OC125 were expressed by different populations of cells. It is concluded that specific combinations of antibodies are more effective both for the monitoring of ovarian cancer as well as for immunodiagnosis and treatment, than any single one used.

Adenocarcinoma↗
PubMed1988

Comparative study of the degree of differentiation of neuroblastoma and mean numbers of nucleolar organiser regions.

Twenty confirmed cases of childhood neuroblastoma diagnosed over six years were reviewed and classified according to the subtyping proposed by Shimada et al. The tumours were stained using a silver colloid method for nucleolar organiser regions (NORs), and the mean number of NORs for every 200 cells was calculated. The correlation between the mean number of NORs and histology and survival was studied. There was a significant correlation between the mean numbers of NORs and differentiation, and with the mitosis-karyorrhexis index (MKI) in the stroma poor group (p = 0.01-0.001). A trend to increased survival with decreased numbers of NORs was observed in the study group as a whole (rank order of correlation = -0.57, p = 0.05-0.02). It is suggested that mean number of NORs is of prognostic value in neuroblastomas.

Cell Count↗
PubMed1988

Ectopic production of beta-human chorionic gonadotropin by inverted papilloma of the nose.

A series of 19 inverted papillomas (transitional type or Schneiderian papillomas) of the nose were examined using an immunogold-silver (IGS) technique and an indirect immunoperoxidase technique. We demonstrated beta-HCG production in all 19 specimens with both methods. The staining demonstrated by the IGS technique was of a greater intensity and in a larger number of cells than that observed by the indirect immunoperoxidase method.

Adult↗
PubMed1988

Long-term follow-up of cyclosporine-treated renal allograft recipients.

Results in 355 Cs-treated renal graft recipients have been analyzed. The majority of graft losses occurred early. Prior sensitization and repeat grafts were clear-cut early risks for cadaver recipients but not live-related donor grafts. In neither group was presensitization clearly a risk factor for late losses, although repeat grafts were much more at risk late. The majority of late losses was due not to chronic rejection but to deaths from cardiovascular disease and cancer, and a surprisingly high number from noncompliance.

Actuarial Analysis↗
PubMed1988

Xanthogranulomatous pyelonephritis. A reappraisal and immunohistochemical study.

The clinicopathologic features of 17 patients with xanthogranulomatous pyelonephritis are described, together with results on a number of histochemical and immunohistochemical techniques that were used to demonstrate the variety of cells involved. Based on our clinicopathologic data and review of the literature, we believe that xanthogranulomatous pyelonephritis should be regarded as a destructive, and, at times, tumefactive inflammatory process that may complicate chronic pyelonephritis. The initiation of this process remains an enigma. However, there appears to be three main features that are associated with xanthogranulomatous pyelonephritis: pelvicalyceal obstruction, ulceration of the pelvicalyceal urothelium, and bacterial infection.

Adult↗
PubMed1987

Primary gastric lymphoma.

This is a population-based review of 153 cases of primary gastric lymphoma. Sixty-seven (43 per cent) were histologically reviewed using the Kiel classification. There were no significant differences between reviewed and unreviewed cases. Ninety-seven per cent of all cases were of the non-Hodgkin's type. The annual incidence was constant at 1.2 per cent of gastric malignancies. The mean age was 60 years and the male to female ratio 1:8. Presenting symptoms were similar to those of gastric cancer. Twenty-one per cent had a palpable mass but one-third of these were amenable to a potentially curative resection. Some 66 per cent were resectable and 58 per cent had a macroscopic clearance of tumour. TNM stage and absolute tumour size were significant prognostic factors (P less than 0.005 and P less than 0.05 respectively) but the Kiel classification was not. The overall 5 year survival was 24 per cent. Apart from 10 patients whose only anti-tumour treatment was radiotherapy (5 year survival 36.9 per cent) no patient who did not have curative surgery survived 5 years. The 5 year survival for curative resection was 34 per cent and for curative resection plus radiotherapy was 43.5 per cent (45 and 73.4 per cent for the node negative cases respectively). A laparotomy is essential; 'curative' surgery possibly with adjuvant radiotherapy offers the best hope for cure.

Adolescent↗
PubMed1987

Nucleolar organizer regions in lymphomas.

Using a silver staining technique, nucleolar organizer region-associated proteins (Ag-NORs) have been studied in paraffin sections of 90 non-Hodgkin's lymphomas, five palatine tonsils and five 'reactive' lymph nodes. The method was readily applicable to these preparations and the Ag-NORs were enumerated with ease. A significant difference was found between the numbers of Ag-NORs in the nuclei of low-grade lymphomas (from a mean of 1 to 1.5 per nucleus) and those of high-grade lymphomas (a mean of 4.4 to 6.8 per nucleus). The Ag-NOR regions were often observed in nuclei in areas where nucleoli themselves were not visible. It is suggested that this method, previously largely the province of the cytogeneticist, should find widespread applications in the field of tumour histopathology.

Humans↗
PubMed1987

Nucleolar organizer regions in small cell tumours of childhood.

A silver colloid technique for nucleolar organizer regions (AgNORs) was applied to paraffin sections of 50 small cell tumours of childhood. These comprised 20 neuroblastomas, 10 Ewing's sarcoma, and 20 rhabdomyosarcomas, including spindle cell, myxoid, and round cell variants. On the basis of the number of AgNORs, one could differentiate between the three groups. The differences observed were statistically significant (P = 0.01-0.001). The subtypes of rhabdomyosarcoma could not be distinguished from one another on the basis of AgNOR counts. We suggest that this simple histochemical technique may be a useful adjunct to the range of special techniques now available to assist the pathologist in the diagnosis of small round cell tumours.

Adrenal Gland Neoplasms↗