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Biomedical subjects

J Colin

Publications and source records attributed to J Colin.

At least 145 records · Page 8Linked to original sources
PubMed1986

Monoclonal antibody analysis of blood and cornea T lymphocyte subpopulations in herpes simplex keratitis.

There is now a great deal of evidence in favor of cell-mediated immunopathogenesis in herpes simplex (HS) keratitis. We used monoclonal antibodies specific for different cell subsets to analyze the blood and cornea-eluded lymphocyte subpopulations in HSV keratitis. There was a significant decrease in the proportion of cells expressing UCHT 1, OKT 4, and MID 4 antigens in the peripheral blood of patients as a whole, as opposed to sex- and age-matched controls. There was a slight decrease in OKT 4 cells in patients with herpetic keratouveitis when compared with patients with herpetic superficial keratitis, and a slight decrease in recurrent herpetic patients. Three corneal buttons showed a marked infiltration by suppressor/cytotoxic T cells, supporting the recent suggestion that lymphocytes might be cytotoxic for keratocytes.

Adult↗
PubMed1986

[Hydrophilic contact lenses and pathogenic microorganisms].

A first study was conducted on 243 hydrophilic contact lenses: 65 were macroscopically abnormal and 100 were infected with fungi. Moreover on Sabouraud's medium with chloramphenicol, 30 bacterial strains were isolated of which 25 Pseudomonas sp. (10 Ps. cepacia) and 1 Serratia liquefaciens. Then a second study was conducted on 107 among the 243 lens solutions: the 50 of the B trade mark were spoiled with 12 X 10(7) bacteria/ml and the 57 of the C trade mark with 5 X 10(7) bacteria/ml. This quantitative bacterial difference was confirmed with a qualitative one: from B trade-mark 6 Pseudomonas aeruginosa strains were isolated and from C only one. The authors emphasize the importance of Ps. aeruginosa in corneal ulcers associated with contact lenses, of lens solution composition, and of preservative usefulness.

Bacteria↗
PubMed1986

Circadian variations of systolic time intervals.

In order to test the reality of a circadian evolution of systolic time intervals, an experiment was conducted on six subjects resting in a supine position for 24 h. Heart rate (HR), systole (S), diastole (D), presystole (PS), isovolumetric contraction time (IVCT), normal and corrected ventricular pre-ejection and ejection time (LPEP, LPEPc, LVET, LVETc) were computed by electric rheoplethysmography every 3 h in supine and sitting positions. Evidence of the existence of a circadian rhythm was clear in supine subjects; especially LPEP and LVET acrophases were, respectively, 4.03 +/- 2.36 h and 4.31 +/- 1.15 h. In sitting subjects, a circadian rhythm was evidenced for IVCT, LVETc and LPEP/LVET, and on the difference of resting-sitting for PS, IVCT and LPEP, suggesting a decrease in orthostatic tolerance at the end of the night. However, values for characteristics of circadian rhythms (mean, amplitude, phase) strongly depend upon experimental conditions (position, activity, diet). A phase difference between systolic times and HR was also observed. This phenomenon was due to an advance in phase of D relative to S. Finally, all these results show that it is always necessary to take circadian factors into consideration during cardiovascular studies, especially for the preparation of protocols and the interpretation of results.

Adult↗
PubMed1986

[Ocular contusion and cellular density of the corneal endothelium].

Specular microscopy was performed on 24 patients with an history of blunt ocular trauma, after a 2 to 35 months follow up. In 29.1% of the patients, there was a cellular loss greater than 5%. No significant association was noted between cell loss and angle recession. This data should be evaluated in medico-legal examinations.

Adolescent↗
PubMed1985

[Phenotype of the mononuclear cells of the cornea in ocular herpes].

There is now a great deal of evidence for cell-mediated immunopathogenesis of herpetic stromal keratitis. However, this has never been unequivocally demonstrated, so far. Accordingly, we established a new technical approach in which lymphocytes were cornea-eluted, further purified and characterized by means of monoclonal antibodies. Four corneal specimens were obtained after penetrating keratoplasty. The patients suffered from herpetic interstitial keratitis with perforation of the cornea. The most relevant point to emerge from this experiment is the marked infiltration of corneal buttons by suppressor/cytotoxic T cells. This finding strongly supports the recent suggestion, by Ahonen and coworkers that lymphocytes might be cytotoxic for keratocytes.

Antibodies, Monoclonal↗
PubMed1985

[Early treatment of ocular herpes simplex. Apropos of 6 cases of herpetic conjunctivitis].

Six patients with previous history of herpetic keratitis were seen during the 48 first hours after the out set of a conjunctivitis. Herpes simplex virus type 1 was isolated in all cases. Treated immediately with trifluorothymidine, they healed quickly without developing any corneal lesions. These data suggest the use of early antiviral treatment in patients with recurrent herpetic keratitis, even preventive therapy in at risk patients in well known predisposing conditions.

Adult↗
PubMed1984

Delayed cerebral pseudo-tumoral radionecrosis following scalp-tumour irradiation. Case report and review of literature.

A case of delayed radionecrosis of the brain is reported, following irradiation of a scalp dermatofibrosarcoma. Signs and symptoms were those of an intracranial neoplasm and appeared 5 years after an irradiation dosage of 1890 rets. Twenty-seven similar cases were gathered from a survey of the literature. Surgical excision was the treatment of choice and led to cure or improvement in 20 cases. Analysis of doses and fractionation revealed excessive irradiation in all but one of the cases.

Adult↗
PubMed1984

[Serologic survey on arbovirus infections in patients presenting with uveitis].

Viruses have been demonstrated or suspected as the causative agents of various types of uveitis. Some arboviruses can be responsible for ocular manifestations. In Brittany, both serosurveys and virus isolations were positive for arboviruses. These results led us to look for the possible role of arboviruses in the aetiology of uveitis. For this, we performed a serosurvey on 131 patients with uveitis of unknown aetiology. Patients were examined in the Ophthalmologic Department of the Brest University Hospital from May 1979 to May 1983. Serum samples were studied: by haemagglutination inhibition (HI) tests against thirteen antigens; by complement fixation (CF) tests against six antigens. The HI and CF tests were performed using micromethods. HI tests revealed antibodies against one or more flaviviruses antigens in thirteen patients: nine monospecific reactions against Yellow Fever virus (as a consequence of a vaccination) and four reactions against several flaviviruses. The four positive reactions are easily explainable by previous trips in countries where several arboviruses are endemic. By CF test we found antibodies at a low titer in two patients: the first one had antibodies against the Zaliv Terpeniya virus and the second against the Avalon virus. Signification of these results is discussed. Our study shows that in Brittany uveitis of unknown aetiology do not seem to be due to arboviruses, at least those tested in this survey or antigenically related.

Adolescent↗
PubMed1984

[Trifluorothymidine therapy of corticoid-treated herpetic keratitis: experimental study in the rabbit].

The combination therapy of corticosteroids and antiviral agent is often necessary in the treatment of different forms of ocular herpes. The association of superficial ulceration with severe stromal inflammation indicates the possible use of ths therapy. An experimental study was performed in rabbits to evaluate if Trifluorothymidine (TFT) used in combination with topical corticosteroids can heal herpetic superficial ulcerations. Biomicroscopic and histopathologic lesions were compared with those of herpetic control eyes, TFT treated eyes and corticosteroid treated eyes. TFT plus corticosteroid treated lesions showed a clinically favourable evolution comparable to that of TFT treated rabbits without corticosteroid. However anatomopathologic examination revealed stromal alterations with herpetic capsids in keratocytes.

Administration, Topical↗
PubMed1984

[Needles used in ocular microsurgery: comparative study using scanning electron microscopy].

The use of microsurgical sutures of various types reveals noticeable differences in the ease of penetration of needles into ocular tissues. In order to try to specify these variations, we have examined different needles used in ophthalmology with a scanning electron microscope: CU 1, CU 5 ( Alcon ), LE 1 (Davis Geck), GS 9, GS 17 (Ethicon), SSC Dm 122 (T ubinger material). The examination was performed on new, unused needles and after having been passed through a cornea five times. Studies on new needles showed certain structural variations. Although the tips had a more or less sharp stem, the edge varied from completely regular to notched. The needle surfaces varied from a very smooth appearance to fluted . After use, the examination reveals the presence of cellular debris. No irregularity was caused by contact with surgical instruments. Comparisons between the imprint and the electron micrographic appearance showed that penetration is clearly better with needles having fluted edges and surfaces.

Cornea↗