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J Cnossen

Publications and source records attributed to J Cnossen.

7 recordsLinked to original sources
PubMed1984

Lymphocyte subpopulations in rheumatoid arthritis. An immunological, enzyme histochemical and morphological study.

Peripheral blood lymphocytes (PBL) and synovial fluid lymphocytes ( SFL ) of patients with rheumatoid arthritis (RA) were examined with monoclonal antibodies, with coated ox red blood cells for the expression of Fc receptors for IgG or IgM (T mu and T gamma cells), and incubated for the demonstration of alpha- naphtyl acetate esterase and acid phosphatase. Equal percentages of OKT4 and OKT8 PBL were found in clinically active and inactive RA patients, and in healthy controls, but decreased percentages of OKT4 and increased percentages of OKT8-positive lymphocytes were found among the SFL . The percentages of T mu and T gamma cells, the presence of HLA-DR membrane antigens on T lymphocytes as well as the staining pattern for the enzymes revealed that SFL of patients with RA were highly activated, compared to PBL of RA patients and healthy controls. It can be concluded from this study that a single determination of OKT4 and OKT8-positive lymphocytes in the peripheral blood of RA patients has no predictive value for disease activity. However, the results of the experiments on T lymphocyte-activation clearly showed preferential activation of SFL compared to PBL, indicating that activation of lymphocytes occurs at the site of inflammation.

Antibodies, Monoclonal↗
PubMed1982

Autoantibodies against Tmu and B lymphocytes in patients with rheumatoid arthritis.

Patients with rheumatoid arthritis have decreased numbers of T mu lymphocytes in their peripheral blood. To find out whether these low number of T mu lymphocytes were associated with the presence of anti-lymphocyte antibodies, the sera of 27 patients with definite or classical rheumatoid arthritis (RA) were investigated for the presence of autoantibodies against subsets of lymphocytes. In addition the numbers of T, T mu, T gamma and B lymphocytes in the peripheral blood of these patients were investigated. Patients with active RA showed lower numbers of T mu lymphocytes in their peripheral blood than patients with inactive RA. However, both groups of RA patients had significantly decreased numbers of T mu lymphocytes in their peripheral blood as compared with 22 age matched healthy donors. Moreover, mainly in patients with active RA cold reactive antibodies were found directed against T mu and B lymphocytes, but never against T gamma lymphocytes of healthy donors. Similar results were found in the indirect immunofluorescence procedure when tested for reactivity against T-cell subsets. This serum reactivity was not caused by rheumatoid factors or antinuclear antibodies. Since RA sera after precipitation with 2.5% polyethyleneglycol, still showed cytotoxicity against T and B lymphocytes, it is suggested that this serum reactivity is not caused by immune complexes but by antibodies.

Adult↗
PubMed1981

Morphological aspects of T cell subpopulations in human blood: characterization of the cerebriform mononuclear cells in healthy individuals.

Cerebriform mononuclear cells (CMC) constitute a morphologically distinct subpopulation of T cells in healthy individuals. They are characterized ultrastructurally by a highly indented nucleus, a high nucleus-to-cytoplasm ratio, condensed chromatin along the nuclear membrane and a scanty cytoplasm. In order to characterize the peripheral blood CMC by enzyme-histochemistry and membrane characteristics, lymphocyte fractions enriched for T cells, T mu cells, T gamma cells and T cells without Fc mu and Fc gamma receptors (T0 cell fraction), or depleted of T cells, were investigated for the presence of alpha-naphthylacetate esterase and acid phosphatase at light and electron microscopic levels. CMC were found exclusively in the T mu-enriched and T0 cell fraction, indicating that these cells have either an Fc mu receptor or no Fc receptor at all. Except for their nuclear indentation, both the CMC in the T mu-enriched fractions and the CMC in the T0 fractions ultrastructurally resembled the characteristic cell in the T mu fraction (TM-type cell), but differed from the characteristic cell in the T gamma fraction (TG-type cell). Moreover, like the TM-type cells all CMC showed paranuclear dots of alpha-naphthylacetate esterase and acid phosphatase activity in their cytoplasm. From these observations it was concluded that CMC without Fc mu receptors were either stimulated T mu cells or precursor T mu cells. Thus CMC in healthy individuals constitute a distinct subpopulation of T cells not only morphologically but also histochemically and immunologically.

Acid Phosphatase↗
PubMed1980

Mixed rosette assay for the detection of T mu and T gamma lymphocytes.

A mixed rosette assay is described for simultaneous detection of T lymphocytes and lymphocytes bearing receptors for the Fc Fragments of IgM (RFc mu) or IgG (RFc gamma) in unfractionated lymphocyte suspensions. For optimal detection of T lymphocytes, treatment of sheep erythrocytes with neuraminidase was necessary. ox erythrocytes labelled with fluorescein isothiocyanate and sensitized with either rabbit IgM or rabbit IgG anti-ox antibodies served as indicator erythrocytes for RFc mu or RFc gamma bearing lymphocytes. In periphral blood the percentage of RFc mu bearing lymphocytes was the same whether determined directly after isolation or after overnight incubation at 37 degrees C. Ratios of non-fluoresceinated sheep erythrocytes to senanti-ox antibodies served as indicator erythrocytes for RFc mu or RFc gamma bearing lymphocytes. In periphral blood the percentage of RFc mu bearing lymphocytes was the same whether determined directly after isolation or after overnight incubation at 37 degrees C. Ratios of non-fluoresceinated sheep erythrocytes to senanti-ox antibodies served as indicator erythrocytes for RFc mu or RFc gamma bearing lymphocytes. In periphral blood the percentage of RFc mu bearing lymphocytes was the same whether determined directly after isolation or after overnight incubation at 37 degrees C. Ratios of non-fluoresceinated sheep erythrocytes to sensitized fluoresceinated ox erythrocytes are critical in the mixed rosette assay; for simultaneous detection of T lymphocytes and RFc mu bearing lymphocytes 1 : 1, and for T lymphocytes and RFc gamma bearing lymphocytes 2 : 1. In both assays the rosette suspension was preincubated for 10 min at 37 degrees C and then centrifuged at 200 X g for 5 min. For optimal simultaneous detection of T lymphocytes and RFc mu bearing lymphocytes incubation at room temperature for at least 2 h was necessary and for T lymphocytes and RFc gamma bearing lymphocytes incubation at room temperature for at least 2 h was necessary and for T lymphocytes and RFc gamma bearing lymphocytes incubation at 4 degrees C for at least 2 h was essential. The percentages of RFc mu or RFc gamma bearing lymphocytes (T mu and T gamma respectively) in the T lymphocyte population determined by mixed rosette assay did not differ significantly from the percentages of RFc mu and RFc gamma bearing lymphocytes in lymphocyte suspensions enriched in T cells by E-rosette sedimentation. The mixed rosette assay is suitable for detection of T mu and T gamma cells in immune deficiency and auto-immune diseases and for analysis of the mononuclear cells in lymphoreticular malignancies.

Animals↗
PubMedDate not supplied

T lymphocyte subpopulations in rheumatoid arthritis.

The number of various lymphocyte subpopulations in the peripheral blood of 28 patients with definite rheumatoid arthritis (RA) were studied. The results were correlated with the disease activity, as assessed by the Ritchie index, erythrocyte sedimentation rate and clinical impression. Patients with active RA showed decreased numbers of T mu lymphocytes, strongly increased numbers of Tnull lymphocytes and slightly increased percentages of Fc gamma lymphocytes as compared to 22 healthy donors. Patients with inactive RA had similar, less striking, but significant changes in T mu and Tnull lymphocytes, but in contrast to patients with active RA had Increased numbers of T gamma lymphocytes. The imbalances in T mu and Tnull cells in patients with RA might be explained by endogenous T mu cell activation, resulting in increased numbers of Tnull cells.

Adult↗