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Biomedical subjects

J Chiba

Publications and source records attributed to J Chiba.

At least 163 records · Page 9Linked to original sources
PubMed1983

Production of monoclonal antibody against a glucosyltransferase of Streptococcus mutans 6715.

A mouse hybrid cell line secreted monoclonal antibody which reacted specifically with Streptococcus mutans 6715 (serotype g) glucosyltransferase (GTase)-synthesizing water-insoluble glucan and inhibited with enzyme reaction. The antibody was cross-reactive with GTase of serotype d but not with GTase of other serotypes of S. mutans when an enzyme-linked immunosorbent assay was used.

Animals↗
PubMed1983

[Effect of islet isograft on diabetes in pancreatectomized rat--long term comparative studies between outbred and inbred animals].

A reproducible model of a diabetic state was produced after resecting more than 90% of pancreatic tissue in Wistar strain male rat (outbred and inbred group). Of a total of 10 animals of outbred group, 6 animals illustrated a blood glucose level over 250 mg/dl in an earlier stage after an injection of 1500 islets into the portal vein. However, in 4 of them blood glucose levels were maintained within normal limit until 4 months. On the other hand, 5 inbred rats in which 500-600 islets were injected demonstrated reversal of diabetes within 1 week after the injection and the effect of the transplantation lasted until about 6 months. A morphometric analysis using 1190 serial histological sections of the control rat pancreas showed that the islet distribution accorded satisfactorily with Weibull or Gamma distribution. The estimated values of size and number of the islet were 0.0155 mm and 131.2/mm3, respectively, while volume ratio of splenic and gastric segments or parabiliary and duodenal segments was estimated to be 1.45 or 0.98%, respectively. The mean size of the isolated islets under dissecting microscope reached 0.080 mm and 500 of them were equivalent to about 10% volume fraction in a whole control pancreas. In the inbred group a transplanted animal contained about 17% of islet volume as compared with control animal.

Animals↗
PubMed1982

Production by cultured spleen cells of inflammatory substances and other lymphokines that mediate delayed-type hypersensitivity in mice.

In vitro cultivation of normal mouse spleen cells with human serum albumin generated effector cells that mediate the delayed-type hypersensitivity (DTH) reaction. The cultured cells, when incubated in a serum-free medium for a further 24 hr, released substances (FPRF) which caused a footpad inflammatory reaction at a maximum of 6 hr after injection into normal syngeneic or allogeneic strains of mice, as well as macrophage migration inhibition factor (MIF) and macrophage activating factor (MAF). The DTH-effector cells in the culture were fractionated in the low density layers by discontinuous bovine serum albumin density gradient centrifugation. The effector cells in the low density layers were further enriched in the Lyt 1 subpopulation of T cells when fractionated on a fluorescence activated cell sorter. Cells capable of producing the inflammatory substances (FPRF), MIF and MAF were also enriched in the same fraction containing DTH-effector cells. These results suggest that low density, Lyt 1-positive T cells mediating the DTH reaction produce FPRF as well as MIF and MAF.

Animals↗
PubMed1982

Contrast thresholds of diabetic patients determined by VECP and psychophysical measurements.

VECPs to checkerboard pattern reversal (12 Hz reversal) at different contrast levels were measured in normal subjects and in patients suffering from diabetes mellitus, with visual acuities above 0.8. VECP contrast threshold was obtained by extrapolating VECP amplitude vs contrast on a log scale to zero microV. Results were compared with psychophysical thresholds. It was shown that both VECP and psychophysical contrast thresholds were significantly higher in the diabetic patients than in the normal subjects.

Adolescent↗