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Biomedical subjects

J Carballo

Publications and source records attributed to J Carballo.

32 records · Page 2Linked to original sources
PubMed1989

Failure of adherence to buccal cells and surface hydrophobicity as virulence markers in Neisseria meningitidis.

Surface hydrophobicity and adherence to buccal epithelial cells were studied in 33 carrier and 34 invasive Neisseria meningitidis strains. It was found that hydrophobicity is statistically similar in both groups (P = 0.0507) although it could be considered that carrier strains are slightly more hydrophobic than invasive ones. Adherence was similar in both groups although more homogeneous in the carrier strains. No correlation could be demonstrated between these two properties nor can they be considered relevant as markers of the carrier or invasive status of this bacterium, indicating that at least in N. meningitidis they are not good properties to discriminate virulent strains.

Bacterial Adhesion↗
PubMed1989

Changes in surface hydrophobicity and charge in Neisseria meningitidis and their correlation with the association to phagocytic cells.

Pairwise correlations among surface charge, relative hydrophobicity and association with phagocytes were studied in two strains of Neisseria meningitidis both under normal conditions and after different surface modifying treatments. The variations in the values of the three variables depended on both strains and treatment, and correlations were highly significant, although the biological significance of these correlations is questionable.

Bacterial Adhesion↗
PubMed1975

Radioimmunoassay of serum pancreatic amylase in normal and pancreatectomized pigs (38504).

A double antibody radioimmunoassay has been developed that is capable of assaying and discriminating small amounts of pancreatic amylase even in the presence of amylase isoenzymes. Assays on adult pigs indicate a mean serum concentration of pancreatic amylase of 2.4mug/ml. To date, no other technique can offer such a discrimination of the pancreatic amylase in the circulation. Following pancreatectomy, the amount of radioimmunoassayable amylase in the circulation disappeared within 48 hr. This was reflected by a simultaneous decline in the serum amylase enzyme activity. Within 48 hr after pancreatectomy, the serum amylase enzyme activity had declined 60%. Thus, in pigs, the pancreas contributes significantly to the circulating amylase levels.

Amylases↗
PubMedDate not supplied

Evaluation of hydrophobicity and adherence of Neisseria meningitidis strains and a study of their correlation by analysis of alterations induced by antibiotics.

Surface hydrophobicity and adherence to human buccal epithelial cells were evaluated in four Neisseria meningitidis strains. Hydrophobicity was measured by partition into p-xylene in the presence or absence of ammonium sulphate, whereas radioactivity-labelled bacteria were used to estimate the ability to adhere to buccal cells by liquid scintillation techniques. Eight antibiotics were employed to induce modifications in the two parameters in order to estimate their possible relationship. The presence of ammonium sulphate enhanced the partition into p-xylene and hence the hydrophobicity values obtained. There was no correlation between adherence and hydrophobicity (no matter what the method employed to evaluate the latter). The effect of the antibiotics varied as a function of the strain, method and/or parameter tested.

Anti-Bacterial Agents↗
PubMedDate not supplied

[Thrombophlebitis of the cavernous sinus secondary to polysinusitis. Report of a new case].

Cavernous sinus thrombophlebitis syndrome is uncommon nowadays. Despite the wide range of antimicrobial agents it carries a high mortality. The disease frequently arises from infection of face, mouth, nose and paranasal sinuses, reaching the cavernous sinus by venous spread. Involvement of adjacent anatomic structures accounts for most of the clinical findings. Meningeal involvement, bilateral orbital edema and ocular cranial nerves palsy are very helpful in the differential diagnosis. Streptococcus spp., Staphylococcus spp., Haemophilus influenzae and anaerobic organisms are considered to be the most frequent etiological agents. In the present paper we report a case of cavernous sinus thrombophlebitis following left side polysinusitis. The patient presented with the main clinical features of the syndrome along with Streptococcus equisimilis and Fusobacterium necrophorum bacteremia. Urgent surgery of infected sinuses and appropriate antibiotic therapy led to a favorable outcome. The patient made and excellent progress and was discharged completely recovered two weeks after admission.

Adult↗
PubMedDate not supplied

Kinetics of alpha-dicarbonyls reduction by L-glycol dehydrogenase (NAD+) from Enterobacter aerogenes.

L-glycol dehydrogenase from Enterobacter aerogenes shows a high affinity by NADH (Ks = 2-4 microM; Km = 4.3-9.7 microM), which indicates that it must operate in vivo saturated with this coenzyme. Michaelis and dissociation constants for the reduction of the carbonyl substrates assayed (diacetyl, 2,3-pentanedione, methylglyoxal and ethyl pyruvate) are similar to those reported for other diacetyl reducing enzymes. The kinetic mechanism followed by these reactions has also been studied. Our results prove that the reduction of diacetyl and ethyl pyruvate takes place via an Ordered Bi-Bi system with the coenzyme as the leading substrate. Methylglyoxal and 2,3-pentanedione are reduced by the same mechanism or by a Theorell-Chance one.

Acetone↗