A NEW SENSITIVE METHOD FOR DETECTING IMMUNOLOGICAL REACTIONS.
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Biomedical subjects
Publications and source records attributed to J CASALS.
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A study by hemagglutination-inhibition test showed that 19 strains of eastern equine encephalitis virus grouped themselves in two main types, which have been designated North American and South American. The former consists of ten strains from the eastern half of the United States, from Massachusetts to Florida; Jamaica, the Dominican Republic, and, subject to confirmation, Thailand. The South American type comprises nine strains from Panama, Trinidad, British Guiana, Brazil, and Argentina. The strains were isolated from different natural hosts over a period of 30 years.
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Owing to the existence of antigenic groups and to the consequent characteristics of the immune response of a host to grouped viruses, certain logical steps are advisable in the process of identification of an arthropod-borne (arbor) virus. The first of these steps is the determination of the arthropod-borne nature of the virus. Since no antigenic property is known to be common to all arbor viruses, the decision is based on other biological properties and on the circumstances of isolation. The second step is determination of the antigenic group. For this are used hyperimmune sera with considerable cross-reactivity within the group, as well as the test that gives the most overlap. The third step is determination of type within the group. Simple immune sera and the least cross-reactive test are used at this step.Viruses that belong in minor groups or are ungrouped often constitute a big problem owing to the fact that, once the major groups have been eliminated from further consideration, comparative studies must be conducted with practically all the remaining viruses before a definitive answer is reached.
A strain of Mayaro virus has been isolated in Trinidad from the mosquito Mansonia venezuelensis. This is the first record of isolation of this agent from naturally infected mosquitoes, caught in the wild.
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