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Biomedical subjects

J C Wilt

Publications and source records attributed to J C Wilt.

At least 37 records · Page 2Linked to original sources
PubMed1975

Immunofluorescence of peritoneal phagocytes after infection of mice with L-cell-attenuated Chlamydia psittaci 6BC.

Large amounts of particulate antigen of Chlamydia psittaci 6BC attenuated by growth in L cells were phagocytized by peritoneal mononuclear phagocytes during the 1st h after intraperitoneal inoculation. The phagocytes subsequently destroyed the immunofluorescent (IF) properties of the chlamydial antigens. It is suggested that the early damage of phagocytes by lysosomal enzymes activation induced by chlamydiae contributed to the relatively early disappearance of IF antigens from the peritoneal fluids.

Animals↗
PubMed1975

Electron microscopy of the in vivo internalization of virulent Chlamydia psittaci 6BC strain.

The internalization of virulent Chlamydia psittaci 6BC particles by wandering mononuclear phagocytes in the peritoneal cavity of intraperitoneally inoculated mice occurred asynchronously, i.e., fragile reticulate bodies (RB) appeared to be more readily phagocytized than the rigid elementary bodies (EB). Early damage of mononuclear phagocytes occurred after internalization of chlamydiae. This was followed by a decreased uptake of particles, and may explain the relatively long persistence (up to 6 h after inoculation) of free, extracellular, "swollen", and RB-like particles. Internalized particles within phagolysosomes showed varying degrees of disintegration. The subsequent influx of polymorphonuclear phagocytes and monocytes into the inflammed peritoneal cavity may explain the rapid disappearance of chlamydiae and their antigens from the peritoneal fluid. The alteration in ultrastructure of peritoneal cells and chlamydial parasites during the inflammatory process are discussed.

Animals↗
PubMed1975

Ultrastructural studies of Chlamydia psittaci 6BC in situ in yolk sac explants and L cells: a comparison with gram-negative bacteria.

Chlamydia psittaci (6BC) was grown in yolk sac explants and in L cells and fixed by perfusion in situ to provide undamaged material for comparison with gram-negative bacteria. Reticulate, intermediate, and elementary bodies were all seen to lack a well-defined periplasmic space; intermediate and elementary bodies showed condensations of the nucleoid which differ from common bacterial configurations; and the cytoplasm of highly condensed elementary bodies was much more electron dense than that of the gram-negative bacteria, while retaining its basically particulate nature. These important morphological distinctions are interpreted as reflections of a significantly different cellular level of organization in these two groups of organisms. No important morphological differences were noted in comparisons of the chlamydial particles grown in the two different host systems.

Animals↗
PubMed1975

The effect of purification on the ultrastructure and infectivity of egg-attenuated Chlamydia psittaci (6BC).

A procedure is described for the purification of mixed populations of the three different morphological forms of Chlamydia psittaci (6BC) from infected yolk sac membranes. Elementary bodies and small intermediate bodies are not perceptibly damaged during purification which involves homogenization of the host cells, differential centrifugation, sedimentation through 20% sucrose, and treatment with trypsin. The observation that elementary bodies undergo plasmolysis in 20% sucrose is interpreted as indicating that the cytoplasmic membrane of these particles is intact at that stage in the purification. Reticulate bodies and large intermediate bodies are damaged, to a degree, by the homogenization step. This damage takes the form of discontinuities of the outer envelope membrane, and results in the loss of the regular coccobacillary shape of these particles and in an increase in their size. Treatment with a combination of RNase and DNase was found to cause profound damage to all three morphological forms of the chlamydiae.

Animals↗
PubMed1971

Psittacosis in Manitoba.

Paired blood specimens submitted to the Virus Laboratory, between January and September 1970, from 200 patients with a lower respiratory tract infection were examined for antibodies to the psittacosis-human pneumonitis group antigen. A high static titre of antibodies was found in four patients and a rising titre in three. The illness varied in the seven patients, tending to be severe, chronic and recurrent. All patients recovered following a course of antibiotic therapy which was repeated in four; treatment tended to be inadequate since the diagnosis of psittacosis was made retrospectively. The likely source of the infection in four of the patients was budgerigars, in one it was pigeons, in one it was a canary as well as a pigeon, and in one the source was not identified. Two additional patients had a fairly high static titre of antibodies to the psittacosis-human pneumonitis antigen but were excluded from this report since they showed a diagnostic increase in antibodies to a respiratory virus during the course of their illness; both patients had an avian contact and are being followed up.

Adolescent↗
PubMed1971

A survey of rubella antibodies in health personnel and a report of vaccine trial.

Five hundred and seventy female health science personnel between the ages of 18 and 25 years were examined for antibodies to the rubella virus by the hemagglutination inhibition technique. Approximately 90% of the subjects had a titre of 1:20 or higher. The geometric mean titre of the positive sera was 1:251. Sixty-four of the 77 persons with an antibody of 1:20 or less volunteered to take the vaccine and were examined six weeks later for the development of antibodies. The conversion rate for the 51 persons who were negative at the dilution of 1:20 at the outset was 94%; the rate of antibody increment for the 13 persons who were positive at 1:20 at the outset was 77%. Among the 51 persons who developed antibodies, the geometric mean titre was 1:57 and among the other 13 it was 1:49. Although the trial was conducted in adult females, the number of side effects from the vaccine was remarkably scanty and insignificant. This trial would seem to emphasize the importance of avoiding the use of rubella vaccine in women of child-bearing age without first excluding pregnancy with meticulous care and using active and controllable contraceptive methods for the two months following vaccination.

Adolescent↗