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Biomedical subjects

J C Rose

Publications and source records attributed to J C Rose.

At least 109 records · Page 6Linked to original sources
PubMed1986

Nifedipine or verapamil counteracts hypertension in gravid ewes.

Hypertension frequently complicates endotracheal intubation in the severely preeclamptic parturient. The calcium entry-blocking drugs nifedipine (N) and verapamil (V) are effective antihypertensive agents in nonpregnant patients. The authors studied the maternal and fetal hemodynamic effects of these drugs in chronically instrumented gravid ewes made hypertensive with an infusion of norepinephrine (NE). Initially NE was infused to increase maternal mean arterial pressure (MAP) by 20%. The NE infusion was continued and either N, 2 mg, or V, 10 mg, was administered intravenously. MAP decreased promptly to control values following both drugs. Maternal heart rate (MHR) decreased significantly following NE infusion. MHR returned to control values following V administration, and increased significantly above control following N administration. Uterine blood flow decreased 50-60% during NE infusion, and there was no further change following either N or V. Fetal hemodynamics were unchanged throughout the study. These results suggest that both N and V may be effective antihypertensive agents in the parturient. V did not produce maternal tachycardia and may be the preferable drug.

Animals↗
PubMed1985

Effect of treatment with sodium valproate on plasma adrenocorticotropic hormone and cortisol concentrations in pregnancy.

To investigate whether treatment with valproic acid in pregnancy decreases adrenocorticotropic hormone and cortisol secretion, we measured their concentrations in maternal and umbilical cord blood from a patient receiving valproic acid. We found them to be normal. Valproic acid may not decrease adrenocorticotropic hormone/cortisol levels in pregnant patients or their fetuses.

Adrenocorticotropic Hormone↗
PubMed1985

Corticotropin-releasing factor--induced adrenocorticotropic hormone release in the sheep fetus: blockade by cortisol.

We administered intravenous injections of synthetic ovine corticotropin-releasing factor to chronically cannulated sheep fetuses and monitored fetal plasma adrenocorticotropic hormone and cortisol concentrations. The three doses of corticotropin-releasing factor used (10, 100, or 1000 ng X kg-1) increased fetal plasma adrenocorticotropic hormone; fetal plasma cortisol levels rose with the highest dose of corticotropin-releasing factor. Administration of corticotropin-releasing factor at these concentrations did not change fetal heart rate or blood pressure. Elevation of fetal plasma cortisol levels to 40 to 80 ng X ml-1 by infusions of the steroid blocked the adrenocorticotropic hormone responses to all three doses of corticotropin-releasing factor. These data indicate that corticotropin-releasing factor can increase plasma adrenocorticotropic hormone concentrations in the late-gestation fetus and that these increases can be blocked by elevations in fetal plasma cortisol levels within a physiologic range. This suggests that cortisol modulates adrenocorticotropic hormone release by the fetal pituitary gland late in gestation.

Adrenocorticotropic Hormone↗
PubMed1985

Gestational age alters fetal breathing response to intravenous insulin and intravenous glucose administration.

An upward change in human maternal plasma glucose concentration is known to increase the percent of time spent in fetal breathing during the late third trimester of human pregnancy. We examined the fetal breathing effects of upward change in plasma glucose (after intravenous glucose administration) and downward change (after intravenous insulin administration) at two different times of day (8 AM and 4 PM) at both 24 and 36 weeks of gestation. No change in the percent of time spent in fetal breathing was seen after insulin infusion. Fetal breathing increased after glucose infusion at 36 weeks of gestation but not at 24 weeks. Responses did not differ between tests performed at 8 AM and 4 PM.

Blood Glucose↗
PubMed1985

Catecholamine responses in fetal lambs subjected to hemorrhage.

We monitored plasma epinephrine and norepinephrine responses to hemorrhage of 20% of estimated blood volume in 11 chronically instrumented, unanesthetized fetal lambs. In addition, we performed control experiments--blood sampling but no hemorrhage--in five fetuses. Arterial blood gases, pH, mean arterial pressures, heart rates, and plasma norepinephrine and epinephrine levels were similar in both groups in the resting state. Arterial blood gases and pH did not change significantly during the experimental period in either group. Mean arterial pressure, heart rate, and plasma norepinephrine and epinephrine levels did not change in the control group during the experimental period. Hemorrhage was associated with a significant decrease in fetal mean arterial pressure, 39.8 +/- 1.2 to 29.6 +/- 2.1 mm Hg, and heart rate, 186 +/- 8 to 146 +/- 6 bpm (p less than 0.01 in both cases). There was a significant increase in plasma norepinephrine, 664.9 +/- 91.6 to 1384.8 +/- 216.7 pg/ml (p less than 0.02) and epinephrine, 224.6 +/- 43.6 to 681.7 +/- 199.0 pg/ml (p less than 0.01) with hemorrhage. These results demonstrate significant catecholamine responses to hypovolemia in the fetal lamb.

Animals↗
PubMed1985

Glucocorticoid receptors in sheep brain tissues during development.

In this study we describe changes in the number of glucocorticoid binding sites that occur in cytosols of pituitary, hypothalamic, and hippocampal tissue obtained from fetal, newborn, and adult sheep. We observed specific, saturable binding of [3H]triamcinolone acetonide by cytosols of all three tissues. There were both age-related and tissue-related differences in the number of binding sites observed. By midgestation (70 days) significant quantities of binding sites were present in pituitary and brain tissues, and the number remained relatively constant throughout gestation and in the neonatal period. The number of binding sites declined markedly in cytosols from adult tissues. At all ages there were more binding sites present in pituitary cytosols than in cytosols of hypothalamus and hippocampus. The sucrose density gradient profile of the radioactive ligand-binding site complex is characteristic of a glucocorticoid receptor. Thus, by midgestation at least a portion of the biochemical machinery mediating glucocorticoid effects is present in the fetal pituitary and in specific brain tissues considered important in the modulation of ACTH secretion by corticosteroids.

Animals↗
PubMed1985

By 94 days of gestation plasma cortisol increases block ACTH response to hypotension in lamb fetuses.

By use of a crossover design, we studied the effects of increasing plasma cortisol concentration on ACTH responses to a standardized stress in 14 lamb fetuses between 94 and 108 days gestation. On a random basis we assigned the animals into two groups of seven. Animals in groups I and II received infusions of cortisol (5 and 1 microgram/min, respectively) or saline for 4 h. After the cortisol or saline pretreatment, we reduced arterial pressure approximately 40-50% in both groups of animals with nitroprusside. After saline pretreatment, hypotension in the group I animals produced an increase in the fetal plasma ACTH from 15 +/- 3 to 200 +/- 20 pg/ml (P less than 0.001), and in the group II animals pretreated with saline plasma ACTH increased from 21 +/- 4 to 141 +/- 19 pg/ml (P less than 0.001) with hypotension. Cortisol pretreatment elevated fetal plasma cortisol levels from 7 +/- 3 to 36 +/- 5 ng/ml in group I and from 8 +/- 4 to 20 +/- 2 ng/ml in group II. The ACTH response to hypotension in both groups was abolished by the cortisol pretreatment. We conclude that by 94 days gestation increases in plasma cortisol within a physiological range block ACTH responses to hypotension in lamb fetuses.

Adrenocorticotropic Hormone↗
PubMed1985

Circadian rhythm of HMG-CoA reductase and insulin in African green monkeys.

HMG-CoA reductase in hepatic microsomes and serum insulin display circadian rhythm in two strains of Cercopethicus aethiops. Grivets develop higher levels of serum cholesterol than vervets fed cholesterol. Males (n = 20/gp) were adapted to a light cycle (7:30 a.m.-8:30 p.m.) for 60 days and fed a non-cholesterol diet at 8:30 a.m. and 2:00 p.m. Vervet acrophase, reductase activity was synchronized to serum insulin units with a specific activity of 1480 pmol/min/mg protein changing by 7.5-fold from nadir to acrophase. The activity profile in grivets was asynchronous to vervet fluctuations with peaks at 12 noon (160 pmol/min/mg) and 6 p.m. (275 pmol/min/mg). Insulin levels also peaked near these times. The 24-h reductase activity was over 4 times greater in vervet than grivet livers. The similar rhythmic patterns of reductase and insulin support the notion that insulin plays an important role in the rhythm of HMG-CoA reductase in primates.

Animals↗
PubMed1985

Halothane effects on subendocardial oxygen supply-demand balance: estimation from intramyocardial tissue pressure and left ventricular pressure.

We evaluated the effect of halothane on the balance of subendocardial oxygen supply and demand in six dogs by estimating the endocardial viability ratio (EVR) based on the pressure generated within the subendocardium (ENDO-IMP). Concurrently, the conventional EVR based on left ventricular pressure (LVP) was estimated and compared with the EVR based on ENDO-IMP. The subendocardial oxygen supply-demand ratio based on ENDO-IMP (IMP-EVR) was significantly less than EVR based on LVP (LVP-EVR) (0.87 +/- 0.03 vs 1.07 +/- 0.06, P less than 0.05) during the control period. With 0.5% halothane administration, IMP-EVR improved significantly (1.04 +/- 0.07, P less than 0.05) while LVP-EVR remained unchanged (1.08 +/- 0.09). No further changes in EVR (either IMP-EVR or LVP-EVR) were observed with increasing halothane concentrations up to 2%. The relationship between the two indices was weak (r2 = 0.44, P less than 0.001) but statistically significant. Because an estimate of EVR based on direct measurement of subendocardial tissue pressure (IMP-EVR) would reflect more accurately the oxygen supply-demand balance of this region than the LVP-EVR, our results suggest that the oxygen balance of the subendocardium improves with halothane administration. The use of LVP-EVR as a hemodynamic index of subendocardial oxygen balance during halothane anesthesia, therefore, is questionable.

Anesthesia↗
PubMed1984

Cortisol infusion blocks adrenocorticotropic hormone but not vasopressin responses to hypotension in fetal lambs.

In eight experiments in which a paired crossover design was used, we studied the ability of physiologic levels of cortisol to block adrenocorticotropic hormone (ACTH) and vasopressin responses to hypotension in fetal lambs. On different days, each fetus received a 4-hour infusion of cortisol or ethanol-saline solution vehicle, and then hypotension was induced with nitroprusside. Mean levels of ACTH before manipulation were 20 +/- 10 pg/ml and 18 +/- pg/ml in the saline solution- and cortisol-treated animals, respectively. Mean values of ACTH increased significantly to 70, 88, and 127 pg/ml at 2.5, 5, and 10 minutes of hypotension after pretreatment with saline solution. Cortisol pretreatment abolished the fetal ACTH response to hypotension. Mean levels of vasopressin during the control period were similar in the two groups of animals (5.7 +/- 1.5 pg/ml versus 5.9 +/- 1.3 pg/ml) and rose to comparable levels (69.4 +/- 15.6 pg/ml versus 65.2 +/- 7.7 pg/ml) during hypotension. Thus, increases in plasma cortisol levels within a physiologic range can suppress hypotension-induced ACTH but not vasopressin release in the fetus.

Adrenocorticotropic Hormone↗
PubMed1984

The chronological distribution of enamel hypoplasias from prehistoric Dickson Mounds populations.

The chronological distributions of enamel hypoplasias (indicators of nonspecific stress) are assessed for 111 individuals from two prehistoric populations from Dickson Mounds, Lewiston, Illinois. The earlier population (circa A.D. 950-1150) involves a transition from an indigenous gathering-hunting tradition to increasing adoption of Mississippian lifeways. The later population (circa A.D. 1150-1300) is fully Mississippian (MM). Based on the occurrence of hypoplasias on all permanent teeth except third molars, 14 half-year periods from birth to 7.0 years are graded for evidence of hypoplasia-stress. Both populations have a low frequency of hypoplasia which occur before 2 years of age and after 4 years of age. A common peak frequency of hypoplasias between 2.0 and 4.0 years is suggestive of an elevated degree of stress at weaning. The peak frequency of hypoplasias is earlier in the MM (2.5-3.0 years versus 3.0-3.5 years in the pre-Mississippian population). In addition, the rise to and decline from peak frequency occurs approximately 0.5 years earlier in the MM. The earlier and sharper rise to peak frequency suggests earlier and more severe weanling-related stress. Hypoplasias chronologies are undoubtedly influenced by age-related host resistance factors (Sarnat and Schour, 1941). Nevertheless, these data demonstrate that populations may vary in their chronological distribution of hypoplasias and that these variations may provide useful information on age-related patterns of exposure to environmental stressors.

Adolescent↗
PubMed1984

Thromboxane and prostacyclin synthesis following whole body irradiation in rats.

The effect of radiation on the mechanism and source of in vivo thromboxane B2 (TxB2) and 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) synthesis was evaluated. Rats were irradiated with 2, 10, or 20 gray (Gy) whole-body gamma irradiation and showed an increase in urine TxB2 after either 10 or 20 Gy. Urine 6-keto-PGF1 alpha was elevated only after exposure to 20 Gy. Irradiation did not alter urine volume or osmolarity, nor was there a correlation between urine osmolarity and the urinary concentration of TxB2 r 6-keto-PGF1 alpha. Rats were pretreated with indomethacin to determine if radiation-induced alterations in urine TxB2 and 6-keto-PGF1 alpha could be suppressed. Pretreatment with indomethacin significantly decreased urine TxB2 and 6-keto-PGF1 alpha in both irradiated and nonirradiated animals. Finally, the sources of urinary cyclooxygenase products were investigated using an isogravitometric cross-perfusion system. These experiments demonstrated that urine TxB2 is derived from extrarenal sources, whereas 6-keto-PGF1 alpha is synthesized primarily by the kidney. It may be concluded that radiation exposure increases in vivo cyclooxygenase pathway activity by both renal and extrarenal tissues.

6-Ketoprostaglandin F1 alpha↗
PubMed1984

Pregnancy alters diurnal variation of plasma glucose concentration.

Human pregnancy introduces a diurnal variation of fasting plasma glucose concentrations, with significantly lower values found after a daytime fast of eight hours compared with a night-time fast of the same duration. The diurnal variation of plasma glucose clearance, found in nonpregnant subjects, is not demonstrable during pregnancy. Insulin tolerance does not exhibit a circadian valuation but is substantially decreased during pregnancy.

Adult↗