Search PubMed⌕ Search

Biomedical subjects

J C Monier

Publications and source records attributed to J C Monier.

At least 181 records · Page 10Linked to original sources
PubMedDate not supplied

[Intranuclear structures of monkey kidney cells recognised by immunofluorescence and immuno-electron microscopy using anti-ribonucleoprotein antibodies (author's transl)].

Autoantibodies which react with a ribonucleoprotein material present in the saline extract of nuclei have been found in the sera of patients with Sharp's syndrome and in some cases of systemic lupus erythematosus and of systemic sclerosis. Such antibodies give a granular or reticulated immunofluorescence staining of the nuclei of mouse peripheral blood leukocytes. When cultures of monkey kidney cells (Macacus cynomolgus) were examined by immunofluorescence using such antibodies, it was found that the staining pattern depended on the state of the cell cultures. Nuclei of cells in stationary cultures showed the presence of perinucleolar granules of RNP whereas, within a few hours after subculture, the nuclei contained widely dispersed specks of antigen. In mitotic cells the antigen appears to be evenly dispersed throughout the cell volume with the exception of the chromosomes. Immuno-electron microscopy of cells treated 24 hours after subculture indicates that the specks correspond to aggregates of 200--250 A granules, probably interchromatin granules.

Animals↗
PubMedDate not supplied

Autoantibodies and human immunodeficiency viruses infection: a case-control study.

OBJECTIVE: To determine the prevalence of organ-specific and non-specific autoantibodies in HIV-infected patients. DESIGN: A multicentric collaborative case-control study including 105 HIV patients and 100 sex- and age-matched HIV-negative healthy volunteers. METHODS: Antinuclear, anti-ds DNA, anti-histone, anti-Sm, rheumatoid factor(IgM), anti-beta 2 glycoprotein 1, antineutrophil cytoplasmic, anti-LKM1, anti-LCA1, anti-gastric parietal cell, antiplatelet, anti-intermediate filament, anti-mitotic spindle apparatus, anti-Golgi, anti-ribosome and anti-thyroid autoantibodies were screened in six European laboratories. RESULTS: Only IgG and IgM anticardiolipin, IgG antiplatelet, anti-smooth muscle and anti-thyroglobulin antibodies were statistically more frequent in HIV patients. There was no correlation with the numbers of CD4+ cells except in the case of anti-smooth muscle antibodies. We were unable to find specific autoantibodies such as anti-ds DNA, anti-Sm, AMA, anti-LKM1, anti-LCA1 or anti-beta 2 GP1 antibodies in these patients. CONCLUSIONS: Our results indicate that the autoantibody profile of HIV infections is comparable to those of other chronic viral infections. HIV does not seem to be more autoimmunogenic than other viruses.

Adult↗
PubMedDate not supplied

Hepatitis C virus infection is frequently associated with high titers of anti-thyroid antibodies.

Objective: To assess the implication of hepatitis C virus (HCV) infection in the development of autoimmune thyroid response, thyroid autoantibodies were studied in serum of HCV positive patients. Methods: Anti-microsomal, anti-thyroperoxidase (anti-TPO) and anti-thyroglobulin antibodies were evaluated in the sera of 100 patients with chronic hepatitis C (53 women and 47 men; mean age = 55&#x00B1;5 years). In parallel, thyroid autoantibodies were investigated in blood samples obtained from two separated control groups: age-matched HCV negative-HBV positive patients (25 women, 25 men; mean age= 47&#x00B1;6 years) and healthy blood donors (29 women and 21 men; mean age= 54&#x00B1;8 years). Results: Anti-thyroid antibodies were found more frequently in HCV positive women when compared to the men (8/53= 15.1&#x0025; vs 0/47= 0&#x0025;,.p<0.01).The prevalence of these autoantibodies was not statistically different between HCV positive and healthy female blood donors. However the investigation of thyroid autoantibody titers showed significantly higher levels of anti-TPO and anti-microsomal antibodies in HCV positive women in comparison with healthy women controls (respectively 1: 83200 vs 1: 1900 and 834 vs 23, p<0,01). Conclusions: This strong association between HCV infection and high levels of anti-thyroid autoantibodies in women outlines the interest of systematic detection of anti-microsomal and/or anti-TPO antibodies in this population.

Journal Article↗
PubMedDate not supplied

[Immune complex disease of Swan and nude mice].

Swan and Nude mice with antinuclear antibodies (AN Ab) show early deposits of Ig and C in the mesangium and the basement membrane of glomerulus, marking an immune complexes disease. We have studied the Ig class of these immune complexes after acid elution and by direct immunofluorescence with suitable conjugates. In the Swan mice, fixed Ig are chiefly IgM and IgA, but AN Ab in the eluate kidney are principally IgG2 and IgM as the circulating AN Ab. In these mice the Ag of the fixed immun complexes are AN and murine leukemia virus Ag. In the Nude mice, fixed Ab are IgM and IgG2 and in part AN Ab. The circulating AN Ab and the AN Ab of eluates are also IgM and IgG2.

Animals↗
PubMedDate not supplied

[A chronological study of thymic crystalline inclusions and autoimmune disorders in Swan and NZB mice (author's transl)].

Swan and NZB mice represent good animal model for human systemic erythematosus with autoimmune phenomena (antinuclear antibodies, deposits of immunoglobulins in kidney and skin). The level of circulating thymic factor, responsible for T lymphocyte differenciation, falls during the first month of life in NZB mice, during the third month in Swan mice, during the sixth month in control mice (Swiss). In Swan mice, cytoplasmic crystalline inclusions have been found within the epithelial cells which are said to have a thymosine-like activity. The time-sequence of the appearance of the crystalline inclusions has been studied in relation to the development of autoimmune disorders (antinuclear antibodies, immunoglobulins in tissues). In each group of mice, the drop in circulating thymic factor appears before the appearance of crystalline inclusions and of autoimmunisation. Therefore, these crystals appear to represent a store of circulating thymosine-like factor or of a precursor in an ageing thymus.

Animals↗
PubMedDate not supplied

Antigenic competition between two sequentially acting antigens. II.--The life-span and sensitivity to corticosteroids or to irradiation of T suppressor cells.

We have studied the immunosuppressive function of T cells using the antigenic competition system. A first antigen (inhibitory antigen) consisting of horse red blood cells is given as a primary or a secondary stimulus. This is followed 4 days later by a second antigen (test antigen) consisting of sheep red blood cells. Both antigens are given intraperitonealy. We have estimated the life-span of T suppressor cells by studying the time necessary for the abolition of antigenic competition after adult thymectomy. We find the response disappears after 6 weeks. The cortico- and radio-sensitivity of T suppressor cells were studied by the effect of hydrocortisone or of irradiation on antigenic competition. Lymphocytes from treated mice were transferred to compatible Nude mice whose ability to show antigenic competition was then studied. By these tichniques we have shown that T suppressor cells are not destroyed by hydrocortisone but their immunosuppressive function is merely temporarily inhibited during the time of high level of this hormone. Furthermore hydrocortisone only inhibits the T suppressor cells when the inhibitory antigen is given as a primary stimulus and shortly after the administration of the steroid. Irradiation on the other hand destroys or inactivates one out two or both cells with a suppressive activity (T suppressor cell and macrophage).

Adrenal Cortex Hormones↗
PubMedDate not supplied

Comparison of three techniques for the detection of antibodies to double-stranded DNA: immunofluorescence on Trypanosoma gambiense, immunofluorescence on Crithidia luciliae and radioimmunoassay using the Farr technique.

Three techniques for the detection of antibodies against double-stranded DNA were compared: two immunofluorescent (IF) techniques using either Trypanosoma gambiense or Crithidia luciliae as antigen and a radioimmunoassay (RIA) based on the Farr technique. The IF on T. gambiense or C. luciliae gives very similar results but the reaction on C. luciliae is easier to read and to interpret. The use of C. luciliae permits the swift elimination of the two main causes of errors: antibodies against the nucleus of Trypanosomidae and antibodies reacting with the base of the flagella. The RIA appears as a very sensitive test since six sera gave a positive reaction with the RIA and a negative reaction with the IF on C. luciliae. However, the RIA gives in nine cases false positive reactions and in four cases false negative results. Those last four sera contained specific antibodies with low avidity for double-stranded DNA.

Animals↗
PubMedDate not supplied

[Humoral and cellular anticollagen autoimmunity in rheumatic pathology].

Using an immuno-enzymatic technique, the authors studied the production of anti-collagen I and II auto-antibodies in 171 cases of rheumatoid arthritis (RA), 40 cases of systemic lupus erythematosus (SLE) and 21 cases of overriding syndrome. Cellular immunity to native collagen II was tested in a group of 26 patients with RA and 27 control subjects, by studying the production of LIF (Leukocyte Inhibitory Factor) in response to this antigen. The anti-collagen I and II antibodies are present in 15.2% and 17.5% of RA patients respectively and also in 15% and 15.5% of the SLE patients studied. Anti-collagen I antibodies are associated with RA with extra-articular signs and anti-collagen II antibodies are associated with early RA, but no association could be found with the clinical or radiological course of the disease. Cellular reactivity to collagen II was found in 30% of the cases of RA studied and was associated with the early forms of the disease. Complementary studies on the association of certain HLA antigens with cellular or humoral reactivity to collagen should allow a better approach to the clinical relationships of certain cases of chronic inflammatory rheumatism with anti-collagen auto-immunization.

Adult↗
PubMedDate not supplied

[Adoptive transfer of immunity against Treponema of Fribourg-Blanc (TFB) in the hamster: role of T lymphocytes (author's transl)].

This adoptive transfer was studied in an inbred strain of syrian hamsters. Spleen and lymph node lymphocytes, from TFB-infected hamsters, were injected to isogeneic recipients. Anti-TFB immunity was studied in the recipients either by looking for the apparition of cutaneous lesions after superinfection by TFB, or by counting of Listeria monocytogenes remaining in the liver and in the spleen 48 h after a mixture of TFB and L. monocytogenes had been inoculated. The first method indicated that a complete inhibition of cutaneous lesions was obtained only with hyperimmunized donors. Our results also indicate that anti-TFB immunity was not medicated by B lymphocytes but by sensitized T lymphocytes.

Animals↗
PubMedDate not supplied

[Detection of anti-collagen type I and II antibodies by an immunoenzymatic technique (ELISA): results in rheumatoid arthritis, systemic lupus erythematosus and leprosy (author's transl)].

An attempt was made to detect antibodies against type I and/or II collagen in sera from patients with rheumatoid arthritis, systemic lupus erythematosus (SLE) and leprae. This study was performed with an immunoenzymatic technique: ELISA (enzyme-linked immunosorbent assay). The following steps were performed: bovine collagen type I or II was adsorbed on glass beads; possible free sites were saturated by incubating the beads with sheep serum; then, the antibodies specifically bound to collagen were detected by a peroxidase-labelled anti-immunoglobulin; the immune complexes at the surface of the beads were revealed by a substrate specific for peroxidase and of great stability: Trinder's reactive. Using conditions previously shown to be optimal, the prevalence of anti-collagen antibodies was as follows. In patients with lepromatous leprae the percentages of positive sera against collagen type I and II were 40% and 44%, respectively; in patients with tuberculoid leprae the percentages were lower: 10% and 30%, respectively. Ten per cent of the SLE patients had antibodies against collagen type I, half of the prevalence noted for anti-collagen type II antibodies (20%). Finally, 13.6% of the patients with rheumatoid arthritis had antibodies against collagen type I, a percentage very similar to that of the patients with anti-collagen type II antibodies (14.6%).

Animals↗